Plasmids and experimental procedures for a visual method
EXPERIMENTAL PROCEDURES
... Scale bar, 10 m. B, flow cytometric quantification of the BiFC signal. Shown are representative runs after transfecting cells with either the SiaT VN and VC plasmids alone or in combination or with only the ...
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EXPERIMENTAL PROCEDURES
... miRNA association with AGO2. Two additional experiments were performed to clarify the effects of PLL and TPF treatment. To assess the activity of PLL on pre-miRNA-Dicer interaction, we checked the abundance of four cell ...
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EXPERIMENTAL PROCEDURES
... Eight-arm Radial Maze—Spatial working memory was measured using an eight-arm radial maze, with each arm radi- ating from an octagonal central arena containing 50 l of water at the end. Several extra maze visual ...
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1. General Experimental Procedures.
... 1. General Experimental Procedures. Proton NMR spectra were recorded on either Bruker 500 MHz or 400 MHz spectrometers. Proton chemical shifts are reported in ppm (δ) relative to internal tetramethylsilane ...
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Visual Tracking: An Experimental Survey
... 7.2 The Methods of Tracking In this paper, trackers are divided according to their main method of tracking. From the papers it is not always clear how the proposed method fits in the history of ideas. As a ...
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Procedures for the Evaluation of the Visual System by Pediatricians
... valid method for screening very young children. 9 A recent randomized, controlled, multicentered crossover study demonstrated photoscreening to be superior to direct testing of visual acuity for screening ...
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A simple method for construction of pir+ Enterobacterial hosts for maintenance of R6K replicon plasmids
... -containing plasmids involve procedures that do not allow selection for presence of the pir gene and require cumbersome and time-consuming screening ...mini-Tn7-based method for rapid and reliable ...
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Visual function in human and experimental glaucoma
... 74 4.1 Introduction The VF test is a standardised test used in vision clinics worldwide, to detect and assess glaucomatous damage. The current, most commonly used testing paradigm, Humphrey’s field analyser (Beck et al. ...
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QuickStep-Cloning: a sequence-independent, ligation-free method for rapid construction of recombinant plasmids
... presented experimental results, it can be claimed that QuickStep-Cloning is a rapid and highly efficient method of molecular ...developed method could be especially useful for protein tagging or, ...
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Visual search behaviour during laparoscopic cadaveric procedures
... different experimental conditions; however the relative nature of such differences was ...The visual search behaviour of two experienced surgeons was recorded as they performed three types of laparoscopic ...
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EXPERIMENTAL PROCEDURES
... A superposition of the relevant residues of the unactivated binary complex PPDC-ThDP (in gray) with the activated ternary com- plex PPDC-3dThDP-POVA (in green and blue for residues of th[r] ...
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EXPERIMENTAL PROCEDURES
... media containing 30 ng/ml recombinant murine soluble recep- tor activator for nuclear factor B ligand. Media were changed every 2 days. TRAP staining was performed on day 5 using a leu- kocyte acid phosphatase kit from ...
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EXPERIMENTAL PROCEDURES
... EXPERIMENTAL PROCEDURES Autospot Peptide Array—Peptide arrays were synthesized on cellulose paper using an Autospot Robot ASP222 or a Multipep * This work was supported by grants from The Norwegian Cancer ...
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Experimental Procedures
... Direct Interaction of the i3 Loop Pepducin Agonist with the H8 Helix of PAR1—In striking contrast, mutation of Gln 379 /Arg 380 and palmitoylated Cys 387 /Cys 388 (44) residues in the H8[r] ...
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EXPERIMENTAL PROCEDURES
... Immunohisto- chemical staining using a new antibody against bovine PMCA4a located the PMCA4a to the apical membrane of the epithelium of cauda epididymidis, whereas testis, caput, and co[r] ...
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EXPERIMENTAL PROCEDURES
... Expression Levels of CatA and CES1 in Clone A and Primary Human Hepatocytes —To assess the involvement of CatA and/or CES1 in the metabolism of PSI-7851, the mRNA and protein expression [r] ...
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EXPERIMENTAL PROCEDURES
... The intercushion fraction was washed and adhered to plas- tic in Dulbecco’s modified Eagle’s medium with 5% fetal bovine serum. The non-adherent fraction was washed, and the adherent KC population was adjusted to 2 ⫻ 10 ...
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EXPERIMENTAL PROCEDURES
... Elevated Levels of Alix Lead to Reduced PDGFR  Down-regulation but Increased c-Cbl Degradation in Response to PDGF-BB—Next, we wanted to determine whether the reduced rate of PDGFR  re[r] ...
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Experimental Procedures
... The following protocol describes a principle procedure to prepare a low volume of a highly concentrated protein formulation to allow for viscosity measurements by a small volume viscometer. Depending on your viscometer ...
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REPORT EXPERIMENTAL PROCEDURES
... 2 The abbreviations used are: PTP, permeability transition pore; pS, picosiemen(s); mCrC, mitochondrial Ca 2⫹ release channel; BN-PAGE, blue native polyacrylamide gel electrophoresis; Cy[r] ...
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