PMA differentiated
Effect of bee venom and its fractions on the release of pro-inflammatory cytokines in PMA-differentiated U937 cells co-stimulated with LPS
... Inflammatory responses mediated by pro-inflammatory cytokines are a key component of protective immunity against many infections. This study shows that treatment of PMA-differentiated U937 cells with BV ...
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III. Ion Channel Expression in PMA-differentiated Human THP-1 Macrophages
... PMA-differentiated THP-1 macrophages (Table). Other investigators have reported up- or down-regulation of I DR in macrophages and related cells. Mouse peritoneal macrophages express I DR after one to four ...
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Metabolomic profiling of the immune stimulatory effect of eicosenoids on PMA-differentiated THP-1 cells
... A growing number of findings highlight the crucial role of metabolic reprogramming in macrophage activation. Immune cells utilise five metabolic pathways: glycolysis, the TCA cycle, the pentose phosphate pathway (PPP), ...
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Effect of melittin on metabolomic profile and cytokine production in PMA-differentiated THP-1 cells
... Melittin shows antiviral, antifungal, antibacterial, and anti-parasitic properties according to several studies and has been proposed to act at cell membrane level [26–28]. However, previous results regarding the effect ...
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Propolis exerts an anti-inflammatory effect on PMA-differentiated THP-1 cells via inhibition of purine nucleoside phosphorylase
... Macrophages, upon stimulation with LPS, perform a multitude of functions for tissue remodelling and immune responses, and they secrete a wide range of factors associated with inflammatory pathways, including ...
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Adenovirus Type 5 Rupture of Lysosomes Leads to Cathepsin B-Dependent Mitochondrial Stress and Production of Reactive Oxygen Species
... million PMA-differentiated THP-1 cells were left untreated, infected with Ad5 at 100,000 ppc, or infected with ts1 mutant adenovirus at 100,000 ppc for 30 ...(B) PMA-differentiated THP-1 cells ...
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Phagocytosis of hemozoin by RAW 264.7 cells, but not THP-1 cells, promotes infection by Leishmania donovani with a nitric oxide-independent mechanism
... and PMA-differentiated THP-1 cells. RAW 264.7 cells and PMA-differentiated THP-1 cells were incubated overnight with increasing amounts of HZ or BH, prior to be washed and re-incubated with ...
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Evidence for IFNα induced, SAMHD1 independent inhibitors of early HIV 1 infection
... 3). PMA-differentiated THP-1 cells (Figure 3A) or divid- ing THP-1 cells (Figure 3B) were treated or not with IFNα for 24 h, and incubated with dN ...in PMA-THP-1 cells in the absence of IFNα ...
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Adenovirus Membrane Penetration Activates the NLRP3 Inflammasome
... NLRP3 activation leads to macrophage necrotic cell death and the release of proinflammatory mediators. Previous re- ports have indicated that infection-induced inflammasome ac- tivation leads to necrotic cell death of ...
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Induction of IFNα or IL 12 depends on differentiation of THP 1 cells in dengue infections without and with antibody enhancement
... and PMA-differentiated THP-1 cells are of the myeloid CD11b + CD11c + and CD14 + , re- sembling their in vivo myeloid counterpart ...hand, PMA-differentiated THP-1 cells, act as mDC, responded ...
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USP18 (UBP43) Abrogates p21-Mediated Inhibition of HIV-1
... in PMA-differentiated THP-1 ...(B) PMA-differentiated THP-1 cells were mock transduced (media) or HIV-1 transduced with or without copackaged ...were PMA differentiated for 48 h ...
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Original Article Comparison of dual source computed tomography (CT) and diffusion-weighted magnetic resonance imaging (MRI) for pathological classification of esophageal cancer
... In addition, this study also analyzed the differ- ential diagnostic value of the NIC value and ADC value against well/moderately differenti- ated esophageal cancer and poorly differenti- ated esophageal cancer through ...
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Interaction between human lung fibroblasts and T-lymphocytes prevents activation of CD4+ cells
... and Anderson [19] and recently modified by Assenmacher et al. [20]. Briefly, Brefeldin A at 10 µ g/mL (Sigma-Aldrich Co., St Louis, MO, USA), was added to cultures and cocul- tures CD4+ T cells and fibroblasts described ...
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Dichloroacetate modulates cytokines toward T helper 1 function via induction of the interleukin-12–interferon-γ pathway
... restored PMA-lowered IFN-γ and IL-12 levels and normalized PMA-increased transforming growth factor-β level, but it inhibited IL-10 levels even further ...
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Method to quantify live and dead cells in multi-species oral biofilm by real-time PCR with propidium monoazide
... (qPCR- PMA) different concentrations of PMA (50, 100 and 200 μM), incubation times in the dark (5, 10, 15 and 30 min) and light exposure times (5, 10 and 30 min) were ...were: PMA 100 μM, incuba- ...
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Effect of the Monocyte Locomotion Inhibitory Factor (MLIF) a Natural Anti Inflammatory Produced by E Histolytica on Apoptosis in Human CD4 + T Lymphocytes
... with PMA, 15 g/ml; with PMA + MLIF, 19 g/ml; and with actinomycin D, 33 g/ml (Figure ...with PMA + MLIF resulted in a there was a statistically significant difference compared to the negative ...
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Induction of the HIV 1 Tat co factor cyclin T1 during monocyte differentiation is required for the regulated expression of a large portion of cellular mRNAs
... using PMA treatment as the dif- ferentiation agent ...of PMA treatment, MM6 cells aggregated and became loosely attached to the bottom of the culture dishes (data not shown), mimicking the differentiation ...
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Detection of viable Plasmodium ookinetes in the midguts of Anopheles coluzzi using PMA qrtPCR
... cells. PMA penetrates damaged cell membranes and intercalates in the DNA inhibiting its PCR ...of PMA-qrtPCR to discriminate between and quantify live and dead Plasmodium berghei malarial parasites and ...
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Evaluation of Prognostic Indicators in Colorectal Carcinoma: A Comprehensive Study to Correlate Staging, Grading and Proliferative Indicators
... The mean Ki67 index for the 14 cases of well-differentiated adenocarcinomas was 54.15% , 16 cases of moderately differentiated carcinomas was 50.83%, for 4 cases of poorly differentiated[r] ...
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Time and Differentiated Integration
... of differentiated integration. To what extent is differentiated integration about temporal differentiation? This general question may be broken down into two more specific concerns with patterns of temporal ...
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