Poly(C) binding protein 1
Original Article Expression of poly(C)-binding protein 1 (PCBP1) in NSCLC as a negative regulator of EMT and its clinical value
... Abstract: Poly (C)-binding Protein 1 (PCBP1) is a 35 kDa protein involved in a number of biological processes. Recently, the research found that PCBP1 might be involved in ...
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Control of mRNA stability contributes to low levels of nuclear poly(A) binding protein 1 (PABPN1) in skeletal muscle
... Nuclear poly(A) binding protein 1 (PABPN1) mRNA is unstable in muscle tissue but stable in cultured ...coactivator 1 α (PGC1 α ) and glyceraldehyde 3-phosphate dehydrogenase (GAPDH), a ...
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RETRACTED ARTICLE: Downregulated Poly-C binding protein-1 is a novel predictor associated with poor prognosis in Acute Myeloid Leukemia
... Quantitative real-time PCR analysis for PCBP1 expression In order to quantify PCBP1 mRNA expression levels, primers 5 ’ -CATCCGCTAAGAATTTAAAAAT-3 ’ (for- ward) and 5 ’ -AAGACAGCAATTCCCAGC-3 ’ (reverse) were used. ...
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Poly(C)-Binding Protein 1, a Novel Npro-Interacting Protein Involved in Classical Swine Fever Virus Growth
... of protein expression ...structural protein expression, we examined CSFV C protein expression when PCBP1 was knocked down in PK-15 ...of C protein com- pared to that in the cells ...
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Replication of Poliovirus Requires Binding of the Poly(rC) Binding Protein to the Cloverleaf as Well as to the Adjacent C-Rich Spacer Sequence between the Cloverleaf and the Internal Ribosomal Entry Site
... the C-rich region is required for RNA replication. Mutations in the C-rich region of the spacer affect the bind- ing of PCBP to the 5 ⴕ -terminal 142 nt of PV ...RNA binding protein PCBP2 ...
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Cellular Poly(C) Binding Proteins 1 and 2 Interact with Porcine Reproductive and Respiratory Syndrome Virus Nonstructural Protein 1β and Support Viral Replication
... PCBP2 protein levels at 72 h post-siRNA treatment in MARC-145 ...kinase 1 (PLK1), silencing of which leads to apoptosis of cells ...of 1 and a later time postinfection (48 h) (data not ...
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Rubella Virus Capsid Protein Interacts with Poly(A)-Binding Protein and Inhibits Translation
... We next investigated whether PABP upregulation occurred in cells that are infected with other RNA viruses. As men- tioned above, it is well documented that PABP is an important FIG. 1. (A) COS cells were ...
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Utilization of Poly(A)-Binding Protein in Monitoring the Formation of mRNP Granules in Toxoplasma gondii
... PABP protein with td- Tomato at the C-terminus, ...and 1 μL of the vector were mixed at room temperature for 10 minutes followed with addition of 1 μL of 25 mM EDTA and incubation for 5 ...
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Poly(A)-Binding Protein Facilitates Translation of an Uncapped/Nonpolyadenylated Viral RNA by Binding to the 3′ Untranslated Region
... mimic poly(A) tail in 3=CITE-dependent ...of poly(A) tail to the 3= terminus stimulated cap-independent translation in the reporter RNA with the core BTE alone but did not in the reporter RNA with the long ...
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Genetic Interactions of Yeast Eukaryotic Translation Initiation Factor 5A (eIF5A) Reveal Connections to Poly(A)-Binding Protein and Protein Kinase C Signaling
... nuclear protein export. To further our understanding of this essential protein, three temperature-sensitive alleles of the yeast TIF51A gene have been character- ...a protein with a single mutation ...
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Hepatitis C Virus Internal Ribosome Entry Site-Dependent Translation in Saccharomyces cerevisiae Is Independent of Polypyrimidine Tract-Binding Protein, Poly(rC)-Binding Protein 2, and La Protein
... in 1 M imidazole in phosphate-buffered saline. Protein concentration was determined by Bradford assay, and equal amounts of protein were loaded on a 10% sodium dodecyl sulfate (SDS)-polyacrylamide ...
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Interplay between the Poly(A) Tail, Poly(A)-Binding Protein, and Coronavirus Nucleocapsid Protein Regulates Gene Expression of Coronavirus and the Host Cell
... the poly(A) tail, N protein, and ...the poly(A) tail, N protein, and PABP regulate gene expression, we first determined whether the poly(A) tail is able to interact with both the N ...
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Functional Characterization of Three Leishmania Poly(A) Binding Protein Homologues with Distinct Binding Properties to RNA and Protein Partners
... RNA binding protein that binds to AU-rich sequences in the 3 ⬘ UTRs of mRNAs, which are selectively destabilized in the trypomastigote stage of the parasite ...PABP2 protein does not shuttle to the ...
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Cellular Protein Modification by Poliovirus: the Two Faces of Poly(rC)-Binding Protein
... lates during the course of infection. This species is not ob- served in the mock-infected samples. The abundance of full- length PCBP also remained constant in the mock-infected samples. These results support the ...
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Human immunodeficiency virus type 1 Rev is required in vivo for binding of poly(A)-binding protein to Rev-dependent RNAs.
... ARRIGO`* Department of Microbiology and Immunology, Medical University of South Carolina, Charleston, South Carolina 29425-2230'; Howard Hughes Medical Institute, School of Medicine, Uni[r] ...
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Rotavirus NSP3 Is a Translational Surrogate of the Poly(A) Binding Protein-Poly(A) Complex
... cellular poly(A) mRNA expres- sion, an MA104 cell line constitutively expressing a destabilized renilla luciferase (24) was ...observed 1 to 2 h after infection, probably due to a transient inhibition of ...
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Extracellular human immunodeficiency virus type 1 Tat protein is associated with an increase in both NF-kappa B binding and protein kinase C activity in primary human astrocytes.
... type 1 (HIV-1) infection has been associated with an increase in the binding of the transcription factor NF- k B to its consensus sequence in the viral ...Tat protein, which has been ...
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Monoclonal Antibodies against the Minimal DNA-Binding Domain in the Carboxyl-Terminal Region of Human Immunodeficiency Virus Type 1 Integrase
... 1% bovine serum albumin) was incubated with endonuclease (Benzonase; 50 U/ml; Sigma) at 37°C for 10 min followed by an extensive wash with buffer B. The immobilized MBP-HIVIN was incubated with MAb 2-19 or ...
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The normal role of Activated Protein C in maintaining homeostasis and its relevance to critical illness
... The inflammatory response also modulates the coagula- tion system, including downregulation of the expression of thrombomodulin (Fig. 3) [9]. Inflammation also increases levels of α 1 -antitrypsin, which is an ...
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The neuronal RNA binding protein HuB as a potential tumor suppressor in glioblastoma
... the C-terminus and is separated from the other two by a highly variable hinge region ...the poly(A) sequence and to preserve the stability of RNA-protein ...the poly(A) tail seems to correlate ...
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