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Polygalacturonase activity

Characterization of an Exopolygalacturonase from Leucoagaricus gongylophorus, the Symbiotic Fungus of Atta sexdens

Characterization of an Exopolygalacturonase from Leucoagaricus gongylophorus, the Symbiotic Fungus of Atta sexdens

... for activity of the enzyme was 60 ˚C, Fig- ure ...of polygalacturonase activity from PGaseLg enzyme showed a typical asymmetric thermal ...in polygalacturonase from ...purified ...

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Preliminary investigations on a polygalacturonase from Aspergillus fumigatus in Chinese Pu’er tea fermentation

Preliminary investigations on a polygalacturonase from Aspergillus fumigatus in Chinese Pu’er tea fermentation

... pectinase activity was assayed from the aspect of polygalacturonase activity, ...enzyme activity was determined by measuring the release of reducing groups using the method of ...

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Application and optimization of solid-state fermentation process for enhancing polygalacturonase production by Penicillium expansum

Application and optimization of solid-state fermentation process for enhancing polygalacturonase production by Penicillium expansum

... whether the salt was removed completely. The target enzyme solution was collected and concentrated in a freeze dryer (Coolsafe110-4; Gene Co., Ltd.). Then the solution was loaded onto a HiTrap CMFF column (5×1 mL, GE ...

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Production, Purification, Characterization And Comparison Of Polygalacturonase From Various Strains Of Aspergillus

Production, Purification, Characterization And Comparison Of Polygalacturonase From Various Strains Of Aspergillus

... adjusted to 4.5 and the medium was sterilized at 121 o C for 15 min . After Sterilization flask were cooled and inoculated with spore suspension of 3-4 day culture of each strain. In order to induce ...

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Molecular Identification of Pectinolytic Streptomyces sp  GHB5 and Optimization of Polygalacturonase Production

Molecular Identification of Pectinolytic Streptomyces sp GHB5 and Optimization of Polygalacturonase Production

... As shown in Figure 3, the level of enzyme production varies with the time duration of the fermentation process [36]. Maximum enzyme yield obtained at 6 days. Data showed that the polygalacturonase activity ...

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Optimization and characterization of polygalacturonase enzyme produced by gamma irradiated Penicillium citrinum

Optimization and characterization of polygalacturonase enzyme produced by gamma irradiated Penicillium citrinum

... Table (4) showed that inoculum size, N content and temperature had significant positive effect on PGase production; however, the interaction between them was not significant. Conversely, pH and incubation period ...

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Solid State Fermentation Process for Polygalacturonase Production Using Cashew Apple

Solid State Fermentation Process for Polygalacturonase Production Using Cashew Apple

... It was observed that initial moisture is the limiting factor in solid state fermentation process, since significant changes in kinetic parameters behaviors are observed with variations of this factor. A challenge to ...

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... of polygalacturonase. 0.5% Polygalacturonase acid was incubating by Polygalacturonase activity (svgma Aldrich, ...export polygalacturonase activity increases initially with the ...

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Microbial Pectinases and their Applications in Industries: A review

Microbial Pectinases and their Applications in Industries: A review

... In cloudy juices, pectic enzymes containing high levels of polygalacturonase activity are added to fruit juices to stabilize the cloud, such as in cloud stabilization of orange juice, le[r] ...

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Biological control of Sclerotium cepivorum Berk  (Onion White Root Rot) using Trichoderma koningii Oudem

Biological control of Sclerotium cepivorum Berk (Onion White Root Rot) using Trichoderma koningii Oudem

... Production of oxalic acid by Sclerotium rolfsii in infected tissue would create an acid environment favourable for polygalacturonase activity as well as sequestering calcium from the pec[r] ...

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Bean Polygalacturonase-Inhibiting Protein Expressed in Transgenic Sugar Beet Inhibits Polygalacturonase from Rhizoctonia Solani

Bean Polygalacturonase-Inhibiting Protein Expressed in Transgenic Sugar Beet Inhibits Polygalacturonase from Rhizoctonia Solani

... degrading polygalacturonase activity of Rhizoctonia solani AG2- 2, the causal agent of the root and stem rot on the sugar ...inhibitory activity has not yet been linked conclusively to the ...

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Pectinase secreted by psychrotolerant fungi: identification, molecular characterization and heterologous expression of a cold-active polygalacturonase from Tetracladium sp.

Pectinase secreted by psychrotolerant fungi: identification, molecular characterization and heterologous expression of a cold-active polygalacturonase from Tetracladium sp.

... Saccharomyces cerevisiae, Candida sp., Debaryomyces sp., Kluyveromyces marxianus, Pichia sp., Saccharomyces sp., Zygosaccharomyces sp., [54], Cryptococcus cylindricus (now Piskurozyma cylindrica), Mrakia frigida, ...

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DISCOVERY OF POLYGALACTURONASE PRODUCING BACILLUS TEQUILENSIS STRAIN ARMATI USING 16S rRNA GENE SEQUENCING

DISCOVERY OF POLYGALACTURONASE PRODUCING BACILLUS TEQUILENSIS STRAIN ARMATI USING 16S rRNA GENE SEQUENCING

... and polygalacturonase. Polygalacturonase is a prime importance for plants since they assist in cell wall extension and softening of some plant tissues during maturation and storage ...[4]. ...

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Constitutive expression of a grapevine polygalacturonase inhibiting protein affects gene expression and cell wall properties in uninfected tobacco

Constitutive expression of a grapevine polygalacturonase inhibiting protein affects gene expression and cell wall properties in uninfected tobacco

... XTH activity, was previously reported to result in a shift towards xyloglu- can with a higher molecular weight, resembling that of older leaves ...XTH activity also increases during fruit ripening and the ...

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Polygalacturonase by spergillus Niger Using Seaweed
Waste Under Submerged Fermentation: Production,
Purification and Characterization

Polygalacturonase by spergillus Niger Using Seaweed Waste Under Submerged Fermentation: Production, Purification and Characterization

... composition. Polygalacturonase had optimum pH at ...acidic polygalacturonase can be used as a potential candidate for clarifying fruit ...[53]. Polygalacturonase made by ...

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OsPGIP1-Mediated Resistance to Bacterial Leaf Streak in Rice is Beyond Responsive to the Polygalacturonase of Xanthomonas oryzae pv. oryzicola

OsPGIP1-Mediated Resistance to Bacterial Leaf Streak in Rice is Beyond Responsive to the Polygalacturonase of Xanthomonas oryzae pv. oryzicola

... To generate the knockout mutation of PG in Xoc, we used the RS105 putative polygalacturonase sequence (GenBank accession No. WP_014503099) to design the primers XocPG-up and XocPG-down (Additional file 2: Table ...

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ISOLATION, CLONING AND PARTIAL CHARACTERIZATION OF THE GENE ENCODING THE POLYGALACTURONASE INHIBITING PROTEIN OF PHASEOLUS VULGARIS CV. NAZ

ISOLATION, CLONING AND PARTIAL CHARACTERIZATION OF THE GENE ENCODING THE POLYGALACTURONASE INHIBITING PROTEIN OF PHASEOLUS VULGARIS CV. NAZ

... with that of Derakhshan cultivar previously reported (Hosseinzadeh Colagar et al., 2004 and accession number AY367002) and PGIP-1 from P.vulgaris cv. Saxa (accession numbers X64769 & A23205) indicates only a single ...

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Molecular study on the extracellular chitinolytic activity and  antifungal activity of bacillus subtilis ts22

Molecular study on the extracellular chitinolytic activity and antifungal activity of bacillus subtilis ts22

... chitinase activity is defined as the amount of enzyme producing 1µ mol reducing sugar per ...enzyme activity was used as Enzyme Activity (U/ml) is equal to change in absorbance per minute x 1000 x ...

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Multiplier Decomposition, Poverty and Inequality in Income Distribution in a SAM Framework: the Vietnamese Case

Multiplier Decomposition, Poverty and Inequality in Income Distribution in a SAM Framework: the Vietnamese Case

... the activity of food processing (A5, act 5) on the same households groups considered above, in order to test which are the relative effects of an industrial activity strictly connected to the agricultural ...

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Gastric ulcer (qarah-e-meda) in unani system of medicine

Gastric ulcer (qarah-e-meda) in unani system of medicine

... antioxidant activity by means of significant free radical scavenging, hydrogen-donating, metal ion chelating, anti-lipid peroxidative and reducing abilities (Visavadiya et ...

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