Polymerase chain reaction analysis
Isolation and expression characterization of CBF2 in vitis amurensis with stress
... Phylogenetic analysis showed that the amino acid sequences may be CBF2 proteins with evolutionary ...reverse-transcription polymerase chain reaction analysis indicated that the ex- ...
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The polymerase chain reaction in the charaterisation and diagnosis of lymphomas
... TCR-y chain genes, notably in B-lym phoblastic ...PCR analysis w ith s ta n d a rd histological assessm ent is essen tial to m inim ise th e risk of m is ...
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Nested polymerase chain reaction technique for the detection of Gpc3 and Afp mRNA in liver cancer micrometastases.
... Some researchers have found that AFP mRNA can be used to detect liver cancer micrometastases. AFP is highly expressed in the fetal yolk sac, but is hardly expressed in healthy adults. However, AFP is expressed during ...
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Development of real time polymerase chain reaction for detection of Salmonella typhimurium and Salmonella enteritidis in fish
... consumption. The food safety analysis includes the presence of pathogens, antibiotics to prev ent aquaculture diseases, and processing activ ities (Hutagalung, 2012). Salmonella spp. is the second m ost c om m on ...
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Polymerase chain reaction on a viral nanoparticle
... the reaction mixture contained only enough DNA to label 4% of pVIII proteins, so that the actual yield of the reaction was ...PAGE analysis of the pVIII protein (Figure ...
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MOLECULAR ANALYSIS OF MLL GENE REARRANGEMENTS IN CHILDHOOD ACUTE LEUKEMIA: A HOSPITAL BASE STUDY
... reverse-transcriptase polymerase chain reaction (PMRT-PCR) technique was performed for molecular ...Data analysis shows no correlation between MLL gene expression and CBC ...
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SCIENTIFIC CONCEPTS OF POLYMERASE CHAIN REACTION (PCR)
... Polymerase Chain Reaction (PCR) is one of the method for the amplification of DNA that has varied applications in many and diverse areas of biological and clinical science 4, 5 ...genetic ...
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Amplification of a species specific DNA fragment of Mycobacterium tuberculosis and its possible use in diagnosis
... Sequence analysis and amplification by polymerase chain reaction of a cloned DNA fragment for identification of Mycobacterium tuberculosis.. de la Maza.[r] ...
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Sequence analysis and amplification by polymerase chain reaction of a cloned DNA fragment for identification of Mycobacterium tuberculosis
... Sequence Analysis and Amplification by Polymerase Chain Reaction of a Cloned DNA Fragment for Identification of Mycobacterium tuberculosis RUBINA J.. WIRTH2* American Registry of Patholo[r] ...
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Degradation of trinitrotoluene by transgenic nitroreductase in Arabidopsis plants
... 1. Polymerase chain reaction (PCR) analysis of the SdNFSBI gene fragment and enhanced TNT (2,4,6-trinitrotoluene) tolerances of plants on plates (a) RT-PCR analysis of the SdNFSBI gene ...
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Reliability of PCR based screening for identification and quantification of GMOs
... use polymerase chain reaction (PCR) based screening methods to monitor the presence of GM particles in food commodities as a cost effective ...PCR analysis clearly showed that, the contami- ...
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A common genetic variation in the 3'-untranslated region of the prothrombin gene is associated with elevated plasma prothrombin levels and an increase in venous thrombosis
... The aim of the present study was to perform an analysis of the prothrombin genes of selected subjects with a history of venous thrombophilia usmg polymerase chain reaction (PCR) and dire[r] ...
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Astrovirus as a possible cause of congenital tremor type AII in piglets?
... and polymerase chain reaction (PCR) analysis and by relating the findings to the occurrence of disease and lesions in the brain, in 4 – 6 days-old piglets obtained from a clinical outbreak of ...
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Diagnosis of tubercular uveitis by quantitative polymerase chain reaction
... The PCR has emerged as a powerful tool for rapid detection of the mycobacterial genome, with a high specificity and a variable sensitivity [3, 9, 11–13]. Aqueous humor, vitreous, or subretinal fluid and even tissue ...
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Molecular Identification of Fowl Adenovirus Associated with Inclusion Body Hepatitis in Iran
... Methods: Polymerase chain reaction (PCR) and sequence analysis of L1 hexon gene was utilized to detect and to determinate the genotypes of Fowl adenovirus (FAdV) in broiler breeder ...
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DNA analysis by polymerase chain reaction of saliva traces on cigarette butts exposed to indoor and outdoor environmental conditions
... Horn, G. T., Richards, B. and Klinger, K.W. (1989). Amplification of a highly polymorphic VNTR segment by polymerase chain reaction. Nucleic Acids Research. 17(5), 2140. National Center for ...
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Sulfur mustard induces expression of metallothionein-1A in human airway epithelial cells
... The polymerase chain reaction conditions comprised primary denaturation at 94 ° C for 5 minutes, fol- lowed by 30 polymerase chain reaction cycles comprising denaturation at 94 ° ...
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Histone H3 lysine 4 methylation is associated with the transcriptional reprogramming efficiency of somatic nuclei by oocytes
... GVs of injected oocytes, fix their contents, including the injected somatic nuclei, and stain by antibodies (Figure 1). This procedure shows the presence of modified his- tones in individual injected nuclei. It therefore ...
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Group specific differentiation between high and low risk human papillomavirus genotypes by general primer mediated PCR and two cocktails of oligonucleotide probes
... sequence analysis, oligonucleotides (30-mers) specific for 19 different HPV genotypes were selected from the internal part of the 150-bp GP5 1 /GP6 1 -amplified ...blot analysis of PCR products derived from ...
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Use of polymerase chain reaction for detection of Chlamydia trachomatis
... Improved sensitivity of a modified polymerase chain reaction amplified DNA probe in comparison with serial tissue culture passage for detection of Chlamydia trachomatis in conjunctival s[r] ...
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