Polymerase Chain Reaction-Restriction Fragment Length Polymorphism"
Polymerase chain reaction – restriction fragment length polymorphism analysis for the differentiation of mycobacterial species in bronchial washings
... Of the 202 specimens, 81 had a growth within eight weeks of incubation. The remaining 121 did not grow. Of the 81, 75 grew within seven days of incubation. Using AFB staining, bacteria (slender, curved rods of pink ...
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Differentiation of canine distemper virus isolates in fur animals from various vaccine strains by reverse transcription polymerase chain reaction restriction fragment length polymorphism according to phylogenetic relations in china
... bp fragment of CDV N gene can be digested into two fragments(659 and 153 bp) in the vaccine strain and three fragments (455, 220 and 153 bp) in the field strains by ...
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Polymorphism in the third intron of the interferonγ gene is associated with susceptibility to multiple sclerosis.
... used polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) to explore the relationship between MS susceptibility and IFN-γ intron III SNPs at the ...
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A Practical Molecular Diagnostic Tool of the Date Moth Ectomyelois ceratoniae (Lepidoptera: Pyralidae) in Tunisia
... pairs fragment of the mitochondrial COI gene from 10 Tunisian ...on Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP), to distinguish ...
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Original Article MMP-2 and MMP-9 gene polymorphisms associated with cervical cancer risk
... Methods: Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method was utilized to examine the frequency and distribution of MMP-2 rs2285053 and ...
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Association between polymorphisms in selected inflammatory response genes and the risk of prostate cancer
... using polymerase chain reaction-restriction fragment length polymorphism technique on 439 prostate cancer patients and 524 controls, and the association of each ...
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A Novel Multiplex PCR-RFLP Method for Simultaneous Genotyping of CYP3A4*4 A>G, CYP3A4*18B G>A and CYP3A4*22 C>T
... multiplex polymerase chain reaction- restriction fragment length polymorphism (PCR-RFLP) method for simultaneous detection of CYP3A4*4 A>G, CYP3A4*18B G>A and ...
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Association study of gene polymorphisms and depression with abnormal humor in traditional Uighur medicine
... the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique was used for genotyping of single nucleotide polymorphisms ...
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Genetic diversity of Mahisefid (Rutilus frisii kutum Kamensky 1901) in different rivers of the south Caspian Sea using PCR-RFLP
... by polymerase chain reaction–restriction fragment length polymorphism (PCR–RFLP) analysis among common carp populations belonging to the European (two farmed strains and ...
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Two single nucleotide polymorphisms in the caprine GnIH gene are associated with litter size . Czech J. Anim. Sci., 62, 269–275.
... Gonadotropin-inhibitory hormone (GnIH) can decrease luteinizing hormone and/or follicle-stimulating hor- mone levels in rat, mouse, sheep, and cattle by the direct suppression of gonadotropin-releasing hormone (GnRH). ...
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40. Identification of species-specific molecular markers in different farm animals by PCR-RFLP analysis
... using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) of a mitochondrial cytochrome b gene (359 ...using restriction enzymes Tas1 and ...
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Molecular Analysis of Leptin Gene Polymorphism in Achai, Sahiwal Cattle and Nili-ravi Buffalo Breeds of Pakistan
... genetic polymorphism in exon III of Leptin gene (LEP) in indigenous animal breeds (Nilli-ravi buffalo, Sahiwal and Achai cattle) of Pakistan using polymerase chain reaction-restriction ...
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Polymorphism of growth hormone gene in three goat breeds in Egypt
... : Polymerase chain reaction; pH: Hydrogen ion concentration of a solution; RFLP: Restriction fragment length polymorphism; rpm: Round per minute; SNP: Single nucleotide ...
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Restriction fragment length polymorphism of polymerase chain reaction products from vaccine and wild-type varicella-zoster virus isolates.
... It is possible that a recombinant virus with the 9 10 both primer pairs of Amplification products of with purified VZV DNA 1 pg from WT, Bgl' WT, and Oka strains lanes 1 through 3, respe[r] ...
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Comparative analysis of human cytomegalovirus a sequence in multiple clinical isolates by using polymerase chain reaction and restriction fragment length polymorphism assays
... HCMV laboratory strains Towne, AD169, and Davis were distinguishable, in terms of size of the amplified product, when analyzed by PCR with primers specific for the a-seq but were indisti[r] ...
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Differentiation of Streptococcus uberis from Streptococcus parauberis by polymerase chain reaction and restriction fragment length polymorphism analysis of 16S ribosomal DNA
... uberis type I ATCC 9927 and type Il ATCC 13386 strains, showing uncut 16S rDNA, a ScaI digest, an NspHI digest, a DdeI digest, and an AvaIt digest.. BstNI-digested pBR322 DNA represents [r] ...
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The involvement of Kras gene 3'-UTR polymorphisms in risk of cancer and influence on patient response to anti-EGFR therapy in metastatic colorectal cancer: a meta-analysis
... PCR, polymerase chain reaction; PCR-RFLP; PCR–restriction fragment length polymorphism; BEL, Belgium Ovarian Cancer study; BWh, Brigham Women’s hospital study; DOV, ...
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Detection by Polymerase Chain Reaction and Restriction Fragment Length Polymorphism of Human Papillomavirus in Archival Cervical Disease
... Detection by Polymerase Chain Reaction and Restriction fragment Length Polymorphism of Human Papillomavirus in Archival Cervical Disease Detec tion by Polymerase Chain Reaction and Restriction fragmen[.] ...
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Identification of verotoxin type 2 variant B subunit genes in Escherichia coli by the polymerase chain reaction and restriction fragment length polymorphism analysis
... The specific activities of extracellular VT2 determined in HeLa cells ranged from 0.3 to 41.7 TCD50 per ,ug of protein in strains carrying the VT2 gene target and from 0 to 50.0 TCD50 pe[r] ...
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Evaluation of Ficolin-3 (FCN3) 1637delC (rs28357092) Frameshift Mutation in Iranian Type 2 Diabetic Subjects
... method, polymerase chain reaction (PCR) was carried out and the restriction fragment length polymorphism (RFLP) method was accomplished using ApaI restriction ...
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