pooled samples
A new approach to epigenome-wide discovery of non-invasive methylation biomarkers for colorectal cancer screening in circulating cell-free DNA using pooled samples
... six pooled samples were measured using the MethylationEPIC ...that pooled serum cfDNA methylation data meets the quality ...the pooled sam- ples. The proportion of CpG detection observed in ...
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Quantitative high resolution melting: two methods to determine SNP allele frequencies from pooled samples
... In order to convert conventional HRM into a quantita- tive assay for pooled samples, equal amounts of each in- dividual’s DNA must be added to the initial asymmetric PCR reaction. This DNA normalization ...
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Increasing Detection and Confirmation of Tuberculosis in Children in Southern Ethiopia: Pooled Samples Tested Using Microscopy and Xpert
... for samples from children increases yield in pooled ...multiple samples of a single child using Xpert MTB/RIF reduce the number of visits re- quired for diagnosis, have the potential to save ...
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PRE-EQC: A Proficiency Testing Program for Pre-Analytical and Analytical Monitoring Using Pooled Samples without Preservatives
... The samples are prepared from excess sample of the laboratory obtained for routine diagnostic ...investigations. Pooled serum/fluoride sample fractions are collected in a sterile plastic container of 50 ml ...
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Comparison of individual and pooled urine samples for estimating the presence and intensity of Schistosoma haematobium infections at the population level
... of samples to be pooled is ≥10 and the number of pools is ...urine samples to the pools reduces as a function of pool size (the total volume of the pools was 50 ml across the pool sizes, and hence ...
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Rapid assessment of faecal egg count and faecal egg count reduction through composite sampling in cattle
... from pooled samples only at D0, higher correlation and agreement coefficients were found between FECR data, the better results being obtained with pools of 5 ...on pooled samples by the ...
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Residual Concentration of PAHs in Seafood from Hormozgan Province, Iran: Human Health Risk Assessment for Urban Population
... eighteen pooled samples of fish, shrimp, crab and bivalve from markets of Hormozgan province, ...investigated samples were classified as minimally ...
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Incidence and knowledge of bovine brucellosis in Kahuro district, Murang’a County, Kenya
... milk samples. Simple random sampling was used to collect two pooled milk samples from each of the 75 milk collection centers in Kahuro ...whose pooled samples gave positive ...
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Prevalence of avian influenza A(H5) and A(H9) in live bird markets in Bangladesh
... Third, our models assumed perfect sensitivity and spec- ificity of real-time RT-PCR for pooled samples. The assays used in this study are considered highly sensitive and specif- ic (27,28), and previous ...
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Analysis of the gut microbiota of walking sticks (Phasmatodea)
... The overabundance of chloroplast contamination greatly reduced the amount of bacterial reads available for ana- lysis. Starving the phasmids for two days was insufficient to clear their digestive tracts. For future ...
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Proposal for field sampling of plants and processing in the lab for environmental metabolic fingerprinting
... In general, the extraction procedure should be quanti- tative for any metabolite in the final sample mixture. In many metabolic fingerprinting or metabolite profiling studies, only one extraction is carried out (see, for ...
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A quantitative study of adipokinetic hormone of the firebug, Pyrrhocoris apterus
... A number of attempts were made to detect endogenous AKH in simple methanolic extracts of haemolymph from bugs. But even in 100 μl of pooled haemolymph, no reliable determinations of the amounts of endogenous AKH ...
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Bacteria of Dental Caries in Primary and Permanent Teeth in Children and Young Adults
... clinical samples were analyzed for 110 bacterial supragingival species in a DNA- DNA hybridization assay (Table ...clinical samples were amplified by PCR with universal primers; the forward primer was ...
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Understanding the impact of antibiotic therapies on the respiratory tract resistome: a novel pooled-template metagenomic sequencing strategy
... the samples individually (although precise costs will be influ- enced by sample number, processing methodologies, and desired sequencing ...between samples from different patients mean that pooling DNA ...
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Staphylococcus aureus antigens reactive with milk immunoglobulin G of naturally infected dairy cows
... aureus-infected cows and the lowest optical densities with milk samples from coagulasenegative staphylococcus-infected cows and uninfected cows were pooled for use as antigen for testing[r] ...
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Sequential determination of serum viral titers, virus specific IgG antibodies, and TNF α, IL 6, IL 10, and IFN γ levels in patients with Crimean Congo hemorrhagic fever
... serum samples obtained from the days 8 and 9 of the illness of a fatal patient who died on day 15 of illness (9 days after hospitalization) showed insufficient IgG levels (Figure ...
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Supragingival plaque microbial analysis in reflection to caries experience
... The present study was performed as part of a series of studies on Saudi mothers and their children. One part aimed at describing the caries experience in relation to various caries-related factors and a clear association ...
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Role of <em>p14<sup>ARF</sup></em> and <em>p15<sup>INK4B</sup></em> promoter methylation in patients with lung cancer: a systematic meta-analysis
... 120 lung cancer samples were included in our analysis. Next, subgroup analyses of the ethnic population (Asians and Caucasians) and sample type (tissue, BALF, and blood) were conducted to find the different ...
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Molecular Bacterial Load Assay Concurs with Culture on NaOH Induced Loss of Mycobacterium tuberculosis Viability
... of samples and storage, a higher-temperature environment may support the growth of fast-growing contami- nants to a concentration which may not be eliminated by NALC-NaOH (25, ...
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Indirect Enzyme Linked Immunosorbent Assay for Detection of Brucella melitensis Specific Antibodies in Goat Milk
... The objective of this study was to develop a sensitive and specific diagnostic test for the detection of B. melitensis anti- bodies in goat milk, with the focused application of testing bulk milk samples. BCSP has ...
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