Position of the stop codon for clone 232
Stop Codon Polymorphism in Two Saccharomyces Species
... 1. ABSTRACT The origin of new coding sequence is a major puzzle in biology. The evolutionary pressures on new sequences are largely unknown, but structural constraints are thought to play a role. Previously, 3' ...
Translation initiation factor eIF3 promotes programmed stop codon readthrough.
... programmed stop codon readthrough. (A) Canonical termination; stop codon in the termina- tion favorable context appears in the A-site (only UAG and UAA stop codons are indicated for ...
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The Attenuated Genotype of Varicella-Zoster Virus Includes an ORF0 Transitional Stop Codon Mutation
... ORF0 stop codon mutation with all three vaccine stock viruses (Table ...a stop codon after residue 129 in the wild-type ...second stop codon at position 221 is reached ...
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Segmentation of DNA into Coding and Noncoding Regions Based on Recursive Entropic Segmentation and Stop Codon Statistics
... com- position as a coding region even though it is a noncoding re- ...same stop-codons com- position as a coding region and because of this, using Ꮽ ...
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Selenium-Dependent Readthrough of the Conserved 3’ -Terminal UGA Stop Codon of HIV-1 Nef
... UGA codon use for Sec, it should not be surprising that viruses might also exploit this mechanism and benefit by the ability to synthesize alternate isoforms of certain viral proteins, ...3 stop codons, ...
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Chikungunya Virus Overcomes Polyamine Depletion by Mutation of nsP1 and the Opal Stop Codon To Confer Enhanced Replication and Fitness
... the stop codon be changed to an arginine for DFMO ...this position confers DFMO resistance (in combination with the XR mutation); thus, SINV may need only this single XR mutation to develop ...
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Increased expression of tryptophan and tyrosine trnas elevates stop codon readthrough of reporter systems in human cell lines
... easily predicted bioinformatically. To overcome this prob- lem, we took advantage of the fact that the endogenous U6 promoter was successfully used for in vivo expression of a suppressor tRNA in the past (56). Therefore, ...
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A Premature Stop Codon in RAF1 Is the Priority Candidate Causative Mutation of the Inherited Chicken Wingless-2 Developmental Syndrome.
... In the transcript studies, whole embryos were examined, such that limb buds were clearly present, although not fully developed (Stage 26 HH, ca. 6 DI) [ 26 ], to study relevant (in terms of the phenotype) early ...
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Local and distant sequences are required for efficient readthrough of the barley yellow dwarf virus PAV coat protein gene stop codon.
... pFNA and pFAS were derived by subcloning BglII-PstI fragments from pPAV GUSRTNA and pPAVGUSRTAS into pFT. pFT was cut with HpaI-NdeI and filled with Klenow DNA polymerase, which fortuitously produced a mutant containing ...
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Pyrrolysine Amber Stop Codon Suppression : Development and Applications
... other stop codon suppression ...the stop codon requirement, whilst other enzymatic methods such as FGE-catalysed elimination frequently require recognition ...amber stop codon ...
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Disruption of the Opal Stop Codon Attenuates Chikungunya Virus Induced Arthritis and Pathology
... opal stop codon at the end of the viral nsP3 ...arginine codon in place of the opal stop codon have been described, and deep-sequencing analysis of a CHIKV isolate from the Caribbean ...
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Versatile Dual Reporter Gene Systems for Investigating Stop Codon Readthrough in Plants
... investigate stop codon readthrough in stably transformed plants, we used a strong constitutive promoter [40] to drive expression of NAN-GUS constructs containing the TMV leaky region in ...of stop ...
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In vitroprediction of stop-codon suppression by intravenous gentamicin in patients with cystic fibrosis: a pilot study
... with stop mutations to chloride-free isoproterenol solution and the nasal cells CFTR surface staining ...for stop mutations with parenteral gentamicin ...with stop mutations had a significantly ...
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Modulation of Stop Codon Read-Through Efficiency and Its Effect on the Replication of Murine Leukemia Virus
... IMPORTANCE Many pathogenic RNA viruses and retroviruses use ribosomal frameshifting or stop codon readthrough to regulate expression of their replicase enzymes. These translational “recoding” processes are ...
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A stop codon polymorphism of Toll-like receptor 5 is associated with resistance to systemic lupus erythematosus
... the stop codon variant is associated with susceptibility to ...TLR5 stop codon polymorphism, but not other TLR5 alleles, is associated with protection from developing ...
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Crystal Structures of the Ribosome in Complex with Release Factors RF1 and RF2 Bound to a Cognate Stop Codon
... the stop codon, we still do not understand the pre- cise structural basis for its recognition and the discrimination against noncognate stop codons as well as sense ...to stop-codon ...
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The 17 Nucleotides Downstream from the env Gene Stop Codon Are Important for Murine Leukemia Virus Packaging
... We have identified a previously unknown nucleotide sequence important for the packaging of murine leukemia virus. This nucleotide sequence is located downstream from the stop codon of the env gene but does ...
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The Relationship Between Third-Codon Position Nucleotide Content, Codon Bias, mRNA Secondary Structure and Gene Expression in the Drosophilid Alcohol Dehydrogenase Genes Adh and Adhr
... of codon bias had no significant effect on average maximum LRT scores (Table 3), nor did it significantly affect the average 5% cutoff ...of codon bias higher than the average from the equal N3 randomiza- ...
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Assessment of Inactivating Stop Codon Mutations in Forty Saccharomyces cerevisiae Strains: Implications for [PSI] Prion Mediated Phenotypes
... The yeast prion [PSI + ] has been implicated in the generation of novel phenotypes by a mechanism involving a reduction in translation fidelity causing readthrough of naturally occurring stop codons. Some [PSI + ] ...
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Assessment of inactivating stop codon mutations in forty Saccharomyces cerevisiae strains: implications for [PSI] prion mediated phenotypes
... The yeast prion [PSI + ] has been implicated in the generation of novel phenotypes by a mechanism involving a reduction in translation fidelity causing readthrough of naturally occurring stop codons. Some [PSI + ] ...
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