Positive-Strand RNA
Importance of the Positive-Strand RNA Secondary Structure of a Murine Coronavirus Defective Interfering RNA Internal Replication Signal in Positive-Strand RNA Synthesis
... MHV-A59-derived RNA, which had the MHV-JHM ...DI RNA (11). A functionally similar RNA element in MHV-A59 DI RNA may be located quite differently from the MHV-JHM 58-nt ...genomic RNA ...
8
Poliovirus CRE-Dependent VPg Uridylylation Is Required for Positive-Strand RNA Synthesis but Not for Negative-Strand RNA Synthesis
... and positive-strand RNA ...poliovirus RNA repli- cation in transfected cells (17); however, the precise defect in viral RNA replication was not ...and positive-strand ...
12
Poly(A) at the 3′ End of Positive-Strand RNA and VPg-Linked Poly(U) at the 5′ End of Negative-Strand RNA Are Reciprocal Templates during Replication of Poliovirus RNA
... negative-strand RNA varied as a function of the size of the 3 ⬘ poly(A) sequences in PV positive-strand RNA templates ...of positive-strand RNA function as ...
16
How Positive-Strand RNA Viruses Benefit from Autophagosome Maturation
... all positive- strand RNA viruses, utilize the maturation of autophagosomes into acidic and ultimately degradative compartments to promote their ...
7
Repair of the tRNA-Like CCA Sequence in a Multipartite Positive-Strand RNA Virus
... the RNA, direct initiation of minus-strand RNA synthesis, promote translation, and con- tribute to increased repair by recruiting cellular enzymes that repair damaged cellular tRNAs (4, 24, 38, ...
11
Poliovirus Requires a Precise 5′ End for Efficient Positive-Strand RNA Synthesis
... both positive- and negative-strand RNA ...XpA- RNA; preinitiation complexes were obtained (see Materials and Methods), resuspended in labeling mix, and further incu- bated at ...RF RNA, ...
7
Multiple Barriers to the Evolution of Alternative Gene Orders in a Positive-Strand RNA Virus
... In this study, we have explored whether the most plausible evolutionary trajectory for the rearrangement of gene order in a positive-strand RNA potyvirus is accessible. Overall, we have identified five ...
29
Capsid Protein Synthesis from Replicating RNA Directs Specific Packaging of the Genome of a Multipartite, Positive-Strand RNA Virus
... two positive-strand RNA molecules (3, ...the RNA- dependent RNA polymerase which directs the replication of RNA1, RNA2, and a 387-nucleotide (nt) subgenomic RNA3 ...of RNA ...
10
Antiviral Innate Immune Response Interferes with the Formation of Replication Associated Membrane Structures Induced by a Positive Strand RNA Virus
... a positive-strand RNA genome establish a membrane-associated replication organelle by hijacking and remodeling intracellular host membranes, a process deemed essential for their efficient ...of ...
11
Intersection of the Multivesicular Body Pathway and Lipid Homeostasis in RNA Replication by a Positive-Strand RNA Virus
... other positive-strand RNA ...for RNA replication by many if not all positive-strand RNA viruses (13, 32, 38, 64, 66, ...entry, RNA replication, and egress ...viral ...
10
Double-Stranded RNA Is Produced by Positive-Strand RNA Viruses and DNA Viruses but Not in Detectable Amounts by Negative-Strand RNA Viruses
... avoid the formation of dsRNA by packaging the genomic and antigenomic RNAs into RNPs. For most other viruses, hiding or sequestering of once-formed dsRNA is the strategy for circumventing activation of the innate immune ...
6
Differential Roles of Hsp70 and Hsp90 in the Assembly of the Replicase Complex of a Positive-Strand RNA Plant Virus
... eukaryotic positive-strand RNA viruses is a regulated process: multiple viral and host components must be assembled on intracellular membranes and ordered into quaternary complexes capable of ...
14
A Positive-Strand RNA Virus with Three Very Different Subgenomic RNA Promoters
... elucidate RNA primary and secondary structural requirements for promoter ...negative- strand-specific conservation of the sgRNA2 promoter second- ary structure suggests its potential importance for ...
9
Mechanisms and consequences of positive strand RNA virus recombination
... analogous events, with the biased output of the CRE-REP assay simply reflecting the absolute requirement for the production of infectious virus particles. While a link between recombination and the generation of DI-RNAs ...
26
Modification of the Untranslated Regions of Human Enterovirus 71 Impairs Growth in a Cell-Specific Manner
... and RNA synthesis in 5=-UTR/IRES recombinant HEV-B and HEV-A viruses than in parental ...and positive-strand RNA synthesis was significantly affected in EVR5⌬Z-infected RD ...
11
Apolipoprotein E but Not B Is Required for the Formation of Infectious Hepatitis C Virus Particles
... HCV RNA) plates were infected with HCV of genotype 2a (JFH1) at 37°C for 2 to 3 ...and positive-strand RNA were detected by Western blotting and an RNase protection assay (RPA), respectively ...
13
Poliovirus cre(2C)-Dependent Synthesis of VPgpUpU Is Required for Positive- but Not Negative-Strand RNA Synthesis
... product RNA. The results presented above suggested that negative-strand RNA was ef- ficiently synthesized in reactions in which the cre(2C)-depen- dent synthesis of VPgpUpU was ...of ...
9
hnRNP A1 interacts with the genomic and subgenomic RNA promoters of Sindbis virus and is required for the synthesis of G and SG RNA
... viral RNA synthesis are not ...SG RNA synthesis during the virus replication ...viral positive strand RNA synthesis, we knocked down the expression of hnRNP A1 in infected cells and ...
7
5′-Proximal Hot Spot for an Inducible Positive-to-Negative-Strand Template Switch by Coronavirus RNA-Dependent RNA Polymerase
... a positive-strand RNA genome and replicate through the use of a 3 ⴕ nested set of subgenomic mRNAs each possessing a leader (65 to 90 nucleotides [nt] in length, depending on the viral species) ...
10
Evidence of Extrahepatic Sites of Replication of the Hepatitis E Virus in a Swine Model
... of positive-strand HEV RNA in each tissue collected during necropsy at different ...negative strand of HEV RNA from tissues that tested positive for the ...
7