Post translational protein modifications
Proteomics of protein post translational modifications implicated in neurodegeneration
... binding protein CBP and other transcriptional coactiva- tors, thereby promoting target transcription and local translation (confirmed by proteomics [142]) required for long term memory ...O-GlcNAc ...
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Analysis of tau post-translational modifications in rTg4510 mice, a model of tau pathology
... The soluble and insoluble fractions of rTg4510 mouse brain were prepared based on a previously published method with some modifications [12]. Briefly, half brains from rTg4510 or wild-type mice were homogenized ...
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Functional decorations: post-translational modifications and heart disease delineated by targeted proteomics
... nated protein can be used for diagnosis), and one or more PADs are either overexpressed or show increased enzymatic activity in other diseases, including multiple sclerosis (myelin basic protein can be ...
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Lysine Post-Translational Modifications of Saccharomyces Cerevisiae Chromatin Proteins
... antibodies are respectively indicated to the left, top, and bottom of the blots. (B) H4 K20me1 is detectable in whole-cell extracts (WCEs) from strains of different genetic backgrounds, mating types, and ploidies, is ...
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A validated antibody panel for the characterization of tau post-translational modifications
... tau modifications and tested their specificity for PTM-modified tau as well as for tau in general using peptide arrays and ...tau protein (hTg-Tau model, [8]) and evaluate the sub- cellular localization of ...
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Role of post translational modifications : glycosylation and palmitoylation in Toxoplasma gondii
... The specific mechanism through which the glycolytic enzymes are associated with the Toxoplasma pellicle is not clear at this time. The observation that association is disrupted at higher KCl concentrations suggests that ...
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A comprehensive catalog of post-translational modifications of Drosophila melanogaster HOX protein, Sex combs reduced
... 1993). Post-transcriptional inhibition of gene expression can be achieved with the RNA interference technique that can act as a reversible switch turning on or off the activity of a gene (Kavi et ...by ...
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Modulation of the Transcriptional Activity of Peroxisome Proliferator Activated Receptor Gamma by Protein Protein Interactions and Post Translational Modifications
... The forkhead transcription factor Foxo1 was identified as a PPARγ-interacting protein that disrupts the binding of PPARγ to the target gene. In addition, PPARγ plays a nega- tive role in the transactivation of ...
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Control of post-translational modifications in antithrombin during murine post-natal development by miR-200a
... on protein expression levels but also in post-translational ...of post-translational modifications of this protein in devel- opmental ...
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Proteomic characterization of novel histone post-translational modifications
... novel modifications using ...MS. Protein kinase C- gamma phosphorylation of this residue increases during apoptosis and any resulting change to nucleosomal structure may promote DNA fragmentation common to ...
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Investigation of post-translational modifications in type 2 diabetes
... Analysis of proteomic data with the help of mass spec- trometry involves extraction of signals from the peptides, identification of peptides with help of search engines to enable the identification of proteins and then ...
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Unrestrictive identification of post-translational modifications in the urine proteome without enrichment
... subject to change, and these proteins may be potential disease biomarkers. As reported previously, in patients with diabetes, there are many advanced glycation end- product peptides in urine [1,2]. The urine ...
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How is epigenetic information maintained through DNA replication?
... histone modifications, which in turn determine the level of com- paction and transcriptional activity of different regions of the ...heterochromatin protein 1 (HP1) to coat and condense ...histone ...
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Functions and regulation of Hdmx and post-translational modifications in drug sensitivity and cancer.
... In untreated His6-SUMO-2 cells, one main SUMO-p53 species is present, likely reflecting SUMOylation at p53-K386, as has been reported (Fig. 1A) 31,37 . Strikingly, treatment with the proteasome inhibitor MG132, thus ...
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Global turnover of histone post-translational modifications and variants in human cells
... heterochromatin protein 1) recognizing methylation on H3K9 would engage in competitive binding against another effector molecular recognizing a phosphorylation on H3S10 ...
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Profiling post-translational modifications of histones in human monocyte-derived macrophages
... Another method of histone preparation is acid extrac- tion. We followed a protocol provided by Abcam, Inc. (http://www.abcam.com/index.html?pageconfig=resource& rid=11410), resulting in approximately four times more ...
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HIV latency reversing agents act through Tat post translational modifications
... Total RNA was extracted from cells using TRIzol (Invit- rogen) following manufacturer instructions followed by RQ1 RNase-Free DNase (Promega, 2 U/μg) treatment for 30 min at 37 °C. One µg of DNase treated RNA was ...
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An integrative analysis of post translational histone modifications in the marine diatom Phaeodactylum tricornutum
... during protein processing and the subsequent serine residue was ...both modifications by western blot in a previous work ...Some modifications were shared only with metazoans and not plants, such as ...
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Conjugative Post Translational Modifications for Pharmacological Improvement of Therapeutic Proteins [Article Retracted]
... a protein, it is necessary the activation of the PEG through a functional group from one of its terminal sides that is suitable for reaction with an available reactive group on the protein (usually the ...
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An investigation into the post-translational modifications of Annexin VI
... this protein, there was no recognizable sequence found in the EMBL ...this protein may be a novel ...binding protein that belongs to the S-100 protein family and has been suggested to play a ...
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