Preparation of Samples from Virus-Infected Cells
Dissemination of Bovine Leukemia Virus-Infected Cells from a Newly Infected Sheep Lymph Node
... when virus-bearing cells first become evident in blood ...fected cells increase sharply, antibodies specific for the SU glycoprotein become detectable in ...lymph samples from ...
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Role of pseudorabies virus glycoprotein gI in virus release from infected cells.
... That deletion of gI affects both virus release and plaque size of the rescued Bartha virus, but does not affect PrV(Ka) in the same way, indicates that the effect of gI on virus release [r] ...
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Shedding of the glycoprotein from vesicular stomatitis virus-infected cells.
... and G proteins of vesicular stomatitis virus suggest that the Gs protein is derived from the G protein by proteolysis.. Both proteins are hydrophobic when analyzed by charge-shift electr[r] ...
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Specific secretion of polypeptides from cells infected with vaccinia virus.
... Order of the samples: A, uninfected cell profile after a 30-min pulse; B, uninfected cell profile after a 30-min pulse in the presence of CAR following treatment for 5 h with 25 pg of CA[r] ...
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Hepatitis C Virus Infection Is Blocked by HMGB1 Released from Virus-Infected Cells
... knockdown cells by treating wild-type HMGB1 for 48 ...transfected cells was reduced by 63 and 39%, respectively, com- pared to that of the control siRNA-transfected ...HCV-infected cells and ...
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Temperature-Sensitive Virus from Aedes albopictus Cells Chronically Infected with Sindbis Virus
... Similarly, small plaque variants became predominant in cultures of Aedes aegypti cells chronically infected with Semliki forest virus (12) and in Aedes albopictus cells chronically infec[r] ...
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A Virus-Encoded RNA Polymerase Purified from Baculovirus-Infected Cells
... fever virus are the only other examples of eukary- otic viruses that encode DNA-dependent RNA polymerases, and their need for a virus-encoded enzyme is usually attributed to the fact that they replicate in ...
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Isolation and characterization of cytoplasmic inclusions from influenza A virus-infected cells.
... Purified inclusions isolated from infected BHK-21-F cells by fluorocarbon extraction and gradient fractionation, negatively stained with uranyl acetate. The high-magnification inset show[r] ...
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Infective substructures of measles virus from acutely and persistently infected cells.
... off35S]methionine- labeled polypeptides obtained from cytoplasmic extracts before sucrose gradient fraction ation: track a, measles virus-infected cells; track b, mock-infected cells. Po[r] ...
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Characterization of polysome-associated RNA from influenza virus-infected cells.
... To assure saturating conditions, increasing concentrations (1 x, 2 x, 3 x, etc.) of unlabeled RNA species (either total cell RNA containing cRNA prepared as above, or unlabeled vRNA extr[r] ...
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Isolation and Properties of an RNA Polymerase from Influenza Virus-Infected Cells
... polymerase activity in fraction 4 from influenza virus-infected BHK21-F cells.. Batches of about 3 X 108 cells were harvested at each time point, and fractions were assayed for RNA polym[r] ...
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Isolation of Herpes Simplex Virus Procapsids from Cells Infected with a Protease-Deficient Mutant Virus
... in infected cells. Particles isolated from m100-infected cells were found to share the defining properties of procapsids assembled in ...resolution from cryoelectron micro- ...
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Temperature-sensitive virus derived from BHK cells persistently infected with HVJ (Sendai virus).
... To isolate infectious virus from BHK-HVJ cells, it was found that the eggs inoculated with test materials were necessary to be incubated at rather lower temperature.. VIROL.[r] ...
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DNA strand exchange catalyzed by proteins from vaccinia virus-infected cells.
... Transfer of a minus strand from the linear duplex DNA to the circular single-stranded viral DNA leads to the formation of joint molecules which can be detected by agarose gel electrophor[r] ...
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Clearance of Measles Virus from Persistently Infected Cells by Short Hairpin RNA
... persistently infected cells were overlaid on uninfected cells, they readily fused ...cured cells prob- ably redistribute CD46 on their surfaces, reachieve the normal even distribution of ...
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Characterization of polypeptides immunoprecipitable from Pichinde virus-infected BHK-21 cells.
... Mock-infected (d) and Pichinde virus-infected (e-n) cell polypeptides were also labeled for 1 h without prior incubation in methionine-free medium and were then treated with normal hamst[r] ...
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Analysis of cytoplasmic RNA and polyribosmomes from feline leukemia virus-infected cells.
... periments with the R-FeLV cDNA were per- formed across polyribosome gradients to corre- late the binding of anti-FeLV to nascent virus- specific protein RNA within polyribosomes. The R-F[r] ...
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Infective Substructures of Sendai Virus from Infected Ehrlich Ascites Tumor Cells
... Increase of infectivity for embryonated eggs was observed in Ehrlich ascites tumor cells after intraperitoneal inoculation of Sendai virus into tumor-bearing mice.. Virus-induced actinom[r] ...
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Role of Proteases in the Release of Porcine Epidemic Diarrhea Virus from Infected Cells
... diarrhea virus (PEDV), a causative agent of pig diarrhea, requires a protease(s) for multicycle replication in cultured ...we infected either Vero cells in the presence of trypsin or Vero ...
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Analysis of herpes simplex virus nucleoprotein complexes extracted from infected cells.
... The cells were labeled from 5 to 18 h after infection with [3H]thymidine, and then extraction, sucrose gradient purification, sample deproteinization, and CsCl centrifugation were perfor[r] ...
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