Primary cilia
Primary cilia maintain corneal epithelial homeostasis by regulation of the Notch signaling pathway
... of primary cilia in the corneal epithelium, we analyzed the ocular surface in mice of different developmental ages by immunofluorescence and transmission electron microscopy ...(TEM). Primary ...
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Primary cilia modulate TLR4-mediated inflammatory responses in hippocampal neurons
... the primary receptor for bacterial LPS, we utilized TLR4 −/− mice to evaluate the effects of LPS on primary ...S2). Cilia lengths were also measured with images accumulated from 2 μm z-stack in total ...
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Characterization of primary cilia during the differentiation of retinal ganglion cells in the zebrafish
... Images were analyzed using Fiji [31]. Primary cilia length in Kupffer’s vesicle was measured manually in maximum intensity z-projections of the confocal stacks. Volume measurements (whole retina and ...
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Characterization of Primary Cilia in Normal Fallopian Tube Epithelium and Serous Tubal Intraepithelial Carcinoma
... of primary cilia on secretory cells and motile cilia on multiple ciliated cells by immunofluorescence and immunohistochemical ...Results: Primary cilia are widespread on secretory cells ...
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Primary cilia regulate Shh activity in the control of molar tooth number
... Primary cilia have recently been shown to play a crucial role in Shh signalling (Rohatgi et ...in cilia, suggesting that the Shh signalling pathway is active in cilia and that cilia are ...
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Current topics of functional links between primary cilia and cell cycle
... of cilia in proliferating cells [35] ...during cilia disassembly as cells re-enter the cell cycle from quiescence: Aurora A-HDAC6-mediated axonemal disassembly is succeeded by Nek2-Kif24-mediated ...
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Gli2a protein localization reveals a role for Iguana/DZIP1 in primary ciliogenesis and a dependence of Hedgehog signal transduction on primary cilia in the zebrafish
... In an earlier study, the sub-cellular distribution of the Igu/DZIP1 protein was analysed using a GFP fusion pro- tein encoded by an mRNA injected into newly fertilised embryos [20]. Following fixation of injected ...
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Models of Distribution of Electric Field of Primary Cilia as Monopole Antennas
... methanol. Primary cilia and cytoplasmic microtubules are stained by mouse monoclonal antibody to acetylated alpha-tubulin (green), centrosome by rabbit antibody to gamma- tubulin (red) and nucleus by DAPI ...
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The Talpid3 gene (KIAA0586) encodes a centrosomal protein that is essential for primary cilia formation
... We have identified a region of the Talpid3 protein that is conserved all the way down to Nematostella vectensis. This conserved region is sufficient for centrosomal localisation and interestingly there is significant ...
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Activation of the Gi protein-RHOA axis by non-canonical Hedgehog signaling is independent of primary cilia.
... abnormal cilia, as a consequence of ciliopathies or oncogenic transformation, since many cancer cells lack primary cilia ...a primary cilium because the basal body is occupied in mitotic ...
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Mechanical signals promote osteogenic fate through a primary cilia mediated mechanism
... of primary cilia ...disrupted primary cilia differentiate to osteoblasts, the absence of primary cilia does not influence osteoblast ...specific primary cilia ...
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Primary cilia control telencephalic patterning and morphogenesis via Gli3 proteolytic processing
... for primary cilia, acting as cellular antennae in sensory perception and ...how primary cilia constrain telencephalic morphogenesis by allowing Gli3R production and hence regulating downstream ...
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Basolateral rather than apical primary cilia on neuroepithelial cells committed to delamination
... the primary cilium after mitosis occurs at the basolateral rather than the apical plasma ...basolateral primary cilia appear at the onset of cortical neurogenesis, increase in abundance with its ...
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Amyloid-β interrupts canonical Sonic hedgehog signaling by distorting primary cilia structure
... torts primary cilia structure, (ii) disrupts canonical Sonic hedgehog (Shh) signaling and (iii) that its precursor, APP, localizes to the primary cilium along with smoothened (Smo) a known ...
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Primary cilia utilize glycoprotein-dependent adhesion mechanisms to stabilize long-lasting cilia-cilia contacts
... the cilia-cilia adhesion, we attempted to dissolve the physical attachments between ...located cilia contacts, moved the transwell to a treatment solu- tion, located the same cells again, and ...
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SPEN, a new player in primary cilia formation and cell migration in breast cancer
... in primary antibodies for 1 h at room temperature. Primary antibodies used were mouse antiace- tylated α-tubulin (1:4000 dilution, clone 6-11B-1; Sigma- Aldrich) and rabbit γ-tubulin (1:1000 dilution; ...
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Primary Cilia Mediate Diverse Kinase Inhibitor Resistance Mechanisms in Cancer.
... of primary cilia in human cancer is ill ...to cilia (Christensen et ...for cilia, or Arl13B, a marker specific for ciliary membranes (Caspary et ...lacked primary cilia, ...
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Role of epidermal primary cilia in the homeostasis of skin and hair follicles
... that cilia on epidermal cells are not essential for ventral or dorsal follicle morphogenesis, but are required for normal homeostasis of the IFE, normal label retention in the bulge and are possibly involved in ...
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Oncometabolite induced primary cilia loss in pheochromocytoma
... of primary cilia in PC12 cells promotes proliferation and alters gene ...of primary cilia. Nuclei were stained with DAPI (blue). Cilia are indicated by arrows, or arrowheads where they ...
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Multiple essential roles for primary cilia in heart development
... of primary cilia in the cbs heart prevents a proper transduction of Shh signaling and thus causes a failure in promotion/maintenance of Bmp4 expression, leading to its loss in the OFT with subsequent conse- ...
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