primary fibroblasts
Highly Efficient Stable Expression of Indoleamine 2,3 Dioxygenase Gene in Primary Fibroblasts
... mouse fibroblasts in the presence of polybrene showed a significant increase in the number of IDO-expressing cells (P ≤ ...IDO-expressing primary fibroblasts ...
6
Large T antigens of simian virus 40 and polyomavirus efficiently establish primary fibroblasts.
... Moreover, the fact that retroviruses encoding the mutant SV-U19 large T antigen established cell lines at least as efficiently as did retroviruses encoding the wild-type large T antigen [r] ...
5
The Role Of Calcineurin/nfat Signaling In Fibroblast Homeostasis And Activation
... from fibroblasts leads to a phenotype that is overall consistent with greater activation “at ...which fibroblasts are studied is still far from the quiescent conditions that would be observed in ...when ...
119
Apicomplexan autophagy and modulation of autophagy in parasite-infected host cells
... gondii is able to induce a significant recruitment of the autophagy machinery in HeLa cells or primary fibroblasts, where genetic inactivation of the autophagic function limits parasite [r] ...
8
MPDU1 mutations underlie a novel human congenital disorder of glycosylation, designated type If
... patients’ fibroblasts accumulated incomplete lipid- linked oligosaccharide precursors for N-linked protein ...in primary fibroblasts of patients restored normal lipid-linked oligosaccharide ...
10
Proapoptotic signaling induced by RIG I and MDA 5 results in type I interferon–independent apoptosis in human melanoma cells
... melanocytes, primary human fibroblasts, or primary human ...for primary cells is shown. *P ≤ 0.05 compared with melanocytes, fibroblasts, or kerati- ...(B) Primary cells were ...
14
A mutation in the NADH-dehydrogenase subunit 2 suppresses fibroblast aging
... from fibroblasts at different time points after doxorubicin treatment until cells developed a senescent phenotype (0 – 8 ...in fibroblasts of Nd2-mutant and control ...Nd2-mutant fibroblasts, but in ...
15
PubMedCentral-PMC4867233.pdf
... In the present study, we describe the role of miR-1343 in attenuating TGF-β signalling and pathways of fibrosis in primary fibroblasts and lung epithelial cell lines. miR-1343 was identified in several ...
27
Primary Tree Shrew Dermis Fibroblasts Support Lytic Replication of Human Cytomegalovirus
... human, primary fibroblasts with skin or lung origin are mostly used to study in vitro HCMV infection because it is one of the main target cell types, easy to culture, permissive for all strains, and ...
10
Human skin equivalents cultured under hypoxia display enhanced epidermal morphogenesis and lipid barrier formation
... of primary fibroblasts and ...of primary cells from human skin tissue originating from surplus breast tissue of healthy .... Primary cells were isolated from surplus human ...
12
Integrated gene and miRNA expression analysis of prostate cancer associated fibroblasts supports a prominent role for interleukin-6 in fibroblast activation
... on fibroblasts activated in vitro with TGFβ or ...activated fibroblasts and superimposable on those found in patient-derived ...IL6-activated fibroblasts but showed an opposite trend in ...activated ...
20
UVB-mediated down-regulation of proteasome in cultured human primary pterygium fibroblasts
... Since the UVB exposure is considered as a main patho- genetic factor in pterygium disease, we examined whether the UVB irradiation has an effect on the expres- sion of proteasome in pterygium fibroblasts, since ...
10
The antifibrotic drug pirfenidone inhibits spondyloarthritis fibroblast-like synoviocytes and osteoblasts in vitro
... In this study, PFD showed both anti-inflammatory and possible anti-fibrotic effects on SpA FLSs. First, the ef- fect of PFD on FLS proliferation and expression of the myofibroblast marker αSMA was studied. PFD inhibited ...
9
Adeno-associated virus vectors transduce primary cells much less efficiently than immortalized cells.
... transduce primary human cells markedly less well than they transduce immortalized ...into primary human fibroblasts did not result in a greater transduction efficiency, indicating that enhanced ...
7
Zannas, Antonios (2017): Epigenetic mechanisms linking psychological stress and aging-related disease. Dissertation, LMU München: Fakultät für Biologie
... embryonic fibroblasts and primary mouse as- trocytes, FKBP51 mediated several effects of paroxetine, namely, decreased the protein-protein interactions of DNMT1 with CDK5 and FKBP52, reduced phosphorylation ...
156
Effect of pH on in vitro biocompatibility of orthodontic miniscrew implants
... on primary human oral gin- gival fibroblasts (HGFs), a permanent human osteogenic sarcoma cell line (U2OS), and a permanent human kera- tinocyte cell line (HaCaT) of four orthodontic mini- implants made of ...
7
Effects of allopurinol and oxipurinol on purine synthesis in cultured human cells
... In the present study we have examined the effects of allopurinol and oxipurinol on the de novo synthesis of purines in cultured human fibroblasts. Allopurinol inhibits de novo purine synthesis in the absence of ...
9
The mechanisms of regulation of Hdm2 protein level by serum growth factors
... Together these data suggest that the primary mechanism underlying the selective up-regulation of Hdm2 protein levels following serum stimulation of human fibroblasts is the well document[r] ...
19
Lodygin, Dmitri (2005): Epigenetic and pharmacological regulation of gene exprssion involved in senescence and tumor progression. Dissertation, LMU München: Fakultät für Biologie
... of primary human cells undergoing replicative senescence was used to gain insight into underlying molecular pathways and to identify a drug-target which can be of use for the inhibition of tumor ...
119
Towards predicting the lung fibrogenic activity of nanomaterials: experimental validation of an in vitro fibroblast proliferation assay
... Since NM400 and NM402 similarly increased fibroblast proliferation and induced similar lung fibrosis, sub- sequent in vitro experiments were mainly performed with NM400. To exclude any FBS-specific effect, e.g. linked to ...
14