Primer Pairs
DETECTION OF VIABLE SALMONELLA TYPHI USING THREE PRIMER PAIRS SPESCIFIC TO INVA, IVAB, AND FLIC-D GENES
... Three primer pairs used in RT-MPCR were invA-f/invA-r, ivaB-f/ivaB-r and fliC-d-f/fliC-d-r that were specific to invA gene, ivaB region and fliC-d gene, ...
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Primer Pairs for Rice (Oryza sativa L.) Bisulfite Sequencing Studies
... c primer pairs to identify and study DNA cytosine methylation, validation studies of NGS uses selective primer ...te primer pairs to selectively study DNA sequences of ...c ...
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Comparison of Flavivirus Universal Primer Pairs and Development of a Rapid, Highly Sensitive Heminested Reverse Transcription PCR Assay for Detection of Flaviviruses Targeted to a Conserved Region of the NS5 Gene Sequences
... different primer pairs, and each result was confirmed three ...the primer pair of Chang (CFDJ 9977 and FUDJ 9166, Table 2), we detected DEN-1, DEN-2, and WN viruses; the Fulop (FG1 and FG2, Table 2) ...
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Anaplasma marginale and A phagocytophilum in cattle in Tunisia
... Methods: In this study a duplex PCR assay was used for the simultaneous detection of Anaplasma marginale and Anaplasma phagocytophilum in cattle using two primer pairs targeting msp4 and[r] ...
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Pandemic Spread of Cholera: Genetic Diversity and Relationships within the Seventh Pandemic Clone of Vibrio cholerae Determined by Amplified Fragment Length Polymorphism
... We have previously shown that ribotype variation is mainly due to changes within the rrn operons that result from recom- bination between the operons (27). This comparison between AFLP and ribotype relationships confirms ...
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Detection and monitoring of hypermethylated RASSF1A in serum from patients with metastatic breast cancer
... different primer pairs flanking different DNA regions of the RASSF1A gene to encounter for the intra- and interbiological variation of the methylation profile to ensure optimal sensitivity and ...the ...
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Assessment of 16S rRNA gene primers for studying bacterial community structure and function of aging flue-cured tobaccos
... optimal primer pairs in 16S rRNA gene sequencing is a pivotal issue in microorganism diversity ...specific primer sets for analysis of the bacterial diversity of aging flue-cured tobaccos (AFTs), as ...
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Development and Validation of a Multiplex PCR for Detection of Scedosporium spp in Respiratory Tract Specimens from Patients with Cystic Fibrosis
... species-specific primer pairs to detect and identify clinically important Pseudallescheria/Scedosporium species in respiratory (sputum) ...the primer design due to its presence as a multicopy gene in ...
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Simian immunodeficiency virus displays complex patterns of RNA splicing.
... Colonies representing the PCR amplification products of the primer pairs PCRAR-PCR25 and PCRSTATPCR25 were initially screened by hybridization with oligonucleotide probes specific for sp[r] ...
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Evolutionary History and Population Dynamics of the M2 Double-stranded RNA of Rhizoctonia solani
... Oligonucleotide primer pairs used to amplify regions of nuclear DNA and mitochondrial large ribosomal (rDNA), and restriction endonucleases used for PCR-RFLP analyses of donor and re[r] ...
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Twenty one new sequence markers for population genetics, species delimitation and phylogenetics in wall lizards (Podarcis spp )
... new primer pairs for polymerase chain reaction (PCR) amplification and sequencing of anonymous sequence markers in Podarcis vaucheri and performed an assay of their cross-amplification and polymorphism ...
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Correlation between DNA methylation and chronological age of Moso bamboo (Phyllostachys heterocycla var. pubescens)
... Recent studies have stated that DNA methylation is closely associated with aging, phase changes in the growth and development processes, and age effects of plants (Finnegan and Kovac 2000; Tariq and Paszkowski 2004; ...
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Sequential Multiplex PCR Approach for Determining Capsular Serotypes of Streptococcus pneumoniae Isolates
... 5 primer set was not included in any of these ...four primer pairs targeting serotype-specific regions of four different serotypes and also included an internal positive control targeting all known ...
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Solitary fibrous tumor in the liver: case report and literature review
... Immunohistochemically, the tumor cells were negative for CD34 (Fig. 3c) but positive for STAT6 (Fig. 3d) and vimentin (Fig. 3e). No markers of HCC (hepatocytes, glypican-3) or GIST (S100 protein, cKIT, DOG1) were ...
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Development of disomic single-locus DNA microsatellite markers for Persian sturgeon (Acipenser persicus) from the Caspian Sea
... 254 primer pairs tested in Lake sturgeon exhibited disomic ...microsatellite primer pairs from a Persian sturgeon enriched microsatellite library (Moghim et ...
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Mega-introns in the Dynein Gene DhDhc7(Y) on the Heterochromatic Y Chromosome Give Rise to the Giant Threads Loops in Primary Spermatocytes of Drosophila hydei
... exon-specific primer pairs, in combination with hybridization experiments with exon- and satellite- specific probes on filters with large PFGE-generated DNA fragments, offer a simple solution for the long- ...
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A quantitative RT-PCR platform for high-throughput expression profiling of 2500 rice transcription factors
... Individual primer pairs can thus exhibit slight differences for their target genes in different ...the primer platform for expression profiling experiments (Caldana et ...
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Simultaneous Detection and Typing of Influenza Viruses A and B by a Nested Reverse Transcription PCR: Comparison to Virus Isolation and Antigen Detection by Immunofluorescence and Optical Immunoassay (FLU OIA)
... Detection limits of the RT-PCR assays. The amplification products of the three outer primer pairs for influenza viruses type A subtype H1, type A subtype H3, and type B were inserted in plasmid pCR 2.1-TOPO ...
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Simultaneous Identification of Mycobacterial Isolates to the Species Level and Determination of Tuberculosis Drug Resistance by PCR Followed by Electrospray Ionization Mass Spectrometry
... 16 primer pairs in eight multiplexed reactions and was validated using a collection of 1,340 DNA samples from cultured specimens collected in the New York City area, the Republic of Georgia, and South ...
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Optimization of quantitative polymerase chain reactions for detection and quantification of eight periodontal bacterial pathogens
... The aim of this study was to optimize quantitative PCR assays (qPCR assays) for 8 important oral bacteria, i.e. Aggregatibacter actinomycetemcomitans, Fusobacter- ium nucleatum, Parvimonas micros, Porphyromonas gin- ...
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