Processing Cell Monolayers from Virus-Infected Cells
Monensin inhibits the processing of herpes simplex virus glycoproteins, their transport to the cell surface, and the egress of virions from infected cells.
... (0) or treated with Electron microscopy of HSV-1-infected HEp-2 I with [35S]methio- cells. As monensin severely inhibited the appear- HSV-1, and then ance of HSV-1 glycoproteins at infec[r] ...
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Nonstructural proteins of Semliki Forest virus: synthesis, processing, and stability in infected cells.
... Labeling conditions: in isotonic medium for 4 min (ns7O); in isotonic medium for 30 min (ns86); window labeling from 6 to 14 (ns6O and ns72). The window labeling procedure yielded an[r] ...
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Quantitative Whole-Cell Proteome Analysis of Pseudorabies Virus-Infected Cells
... extracts from labeled (mock-infected) and unlabeled (PrV-infected) cells at 1:1 protein ratios was then used for the parallel extraction of phosphoproteins with a commercially available ...
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Cell-to-Cell Contact with Hepatitis C Virus-Infected Cells Reduces Functional Capacity of Natural Killer Cells
... if cell-free HCV particles may affect NK cell function in a specific micromilieu that immobilizes and concentrates the virions and extracellular NS5A may suppress NK cells after release from ...
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Specific secretion of polypeptides from cells infected with vaccinia virus.
... Order of the samples: A, uninfected cell profile after a 30-min pulse; B, uninfected cell profile after a 30-min pulse in the presence of CAR following treatment for 5 h with 25 pg of CA[r] ...
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Translation of mRNA from Simian Virus 40-Infected Cells into Simian Virus 40 Capsid Protein by Cell-Free Extracts
... RNA, isolated from cells late in infection by SV40, can direct the synthesis of a protein which closely resembles the major SV40 capsid protein in cell-free extracts from both CHO and re[r] ...
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Polarization of Allogeneic T-Cell Responses by Influenza Virus-Infected Dendritic Cells
... mice infected with influenza virus has both Th1- and Th2-type ...antigen-presenting cells and T cells is one mechanism that may alter the quality of the immune ...dendritic cells (DC) ...
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Mature Dendritic Cells Infected with Canarypox Virus Elicit Strong Anti-Human Immunodeficiency Virus CD8+and CD4+ T-Cell Responses from Chronically Infected Individuals
... T cells and recall CTL responses in the absence of repetitive stimulation or cytokines, which are traditionally used to expand HIV-specific CD8 ⫹ effector cells in ...canarypox virus-infected ...
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Dissemination of Bovine Leukemia Virus-Infected Cells from a Newly Infected Sheep Lymph Node
... of cells and antibodies in dripping ...with cell-free maedi-visna virus, and SU antibodies followed a week later ...case, virus-infected cells disseminate in lymph along with ...
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Human Dendritic Cells Infected with the Nonpathogenic Mopeia Virus Induce Stronger T-Cell Responses than Those Infected with Lassa Virus
... resulted from the different types of type I IFN responses induced by these two ...T cells with MV-infected iDC (data not ...T cells showed equivalent proliferation 5 days after their ...
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Characterization of polysome-associated RNA from influenza virus-infected cells.
... To assure saturating conditions, increasing concentrations (1 x, 2 x, 3 x, etc.) of unlabeled RNA species (either total cell RNA containing cRNA prepared as above, or unlabeled vRNA extr[r] ...
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Transcriptome analysis of pig intestinal cell monolayers infected with Cryptosporidium parvum asexual stages
... host cell remains challenging. Based on an RNA-Seq analysis of monolayers of pig epithelial cells infected with ...of infected and uninfected monolayers demonstrates a ...
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Identification of an FMR cell surface antigen associated with murine leukemia virus-infected cells.
... In addition, the G anti- (F)MuLV and EdG2 leukemia cells contained on serum precipitated an 85,000-dalton protein their cell surface the viral envelope protein gp7O. from the cell extrac[r] ...
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Targeting Hepatitis B Virus-Infected Cells with a T-Cell Receptor-Like Antibody
... Splenocytes from immunized animals were fused with NS1 myeloma cells at a 1:1 ratio, and super- natants of the resulting hybridomas ( ⬃2,000 hybridomas) were screened by using HLA-A201 ⫹ T2 cells ...
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Characterization of hepatitis C virus replication in cloned cells obtained from a human T-cell leukemia virus type 1-infected cell line, MT-2.
... leukemia virus type 1-infected cell line, MT-2, could support the replication of hepatitis C virus (HCV) ...MT-2 cell lines by the limiting dilution ...in infected cells ...
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Remote Activation of Host Cell DNA Synthesis in Uninfected Cells Signaled by Infected Cells in Advance of Virus Transmission
... uninfected cell is modified by exposure to infected ...work from high-multiplicity analysis convincingly shows that HSV actively blocks various stages of the cell cycle, including G 1 -S ...
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Clearance of Measles Virus from Persistently Infected Cells by Short Hairpin RNA
... complexes. Cell surface reorganization of the MV re- ceptor CD46 plays an important role in this ...persistently infected cells were overlaid on uninfected cells, they readily fused ...cured ...
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Characterization of polypeptides immunoprecipitable from Pichinde virus-infected BHK-21 cells.
... Mock-infected (d) and Pichinde virus-infected (e-n) cell polypeptides were also labeled for 1 h without prior incubation in methionine-free medium and were then treated with normal hamst[r] ...
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Cell Fusion by Canine Distemper Virus-Infected Cells
... Since intact virus-infected cells lost their ability to cause cell fusion when treated in this. manner, it seems likely that disruption of cells[r] ...
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Role of pseudorabies virus glycoprotein gI in virus release from infected cells.
... That deletion of gI affects both virus release and plaque size of the rescued Bartha virus, but does not affect PrV(Ka) in the same way, indicates that the effect of gI on virus release [r] ...
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