Proportion of Denaturing Gradient Gel Electrophoresis (DGGE) profiles
Statistical Analyses of Complex Denaturing Gradient Gel Electrophoresis Profiles
... banding profiles, each of which an- swer different and progressively more complex ...the profiles of both cohorts but can also specify individ- ual operational taxonomic units associated with these differ- ...
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Denaturing Gradient Gel Electrophoresis (DGGE)
... information Denaturing gradient gel electrophoresis (DGGE) is a molecular fingerprinting method that separates polymerase chain reaction (PCR)-generated DNA ...agarose gel ...
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Denaturing Gradient Gel Electrophoresis as a Diagnostic Tool in Periodontal Microbiology
... of denaturing gradient gel electrophoresis (DGGE) as a tool for the detection of clinically relevant species and to compare the results of detection by DGGE with those by PCR and ...DGGE ...
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Evaluation of denaturing gradient gel electrophoresis (DGGE) as a method of mutation detection
... The a sso cia tio n w ith p a rtic u la r m utations and d iffe re n t m utant haplotypes suggests that there has been m ultiple fo u n d in g p o p u la tio n s for the PAH m u ta tio n s. These observations suggest ...
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Denaturing gradient gel electrophoresis as a fingerprinting method for the analysis of soil microbial communities
... molecules, denaturing gradient gel electrophoresis (DGGE) or temperature gradient gel electrophoresis (TGGE), are the most widely ...
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Diversity of Hepatitis C Virus Quasispecies Evaluated by Denaturing Gradient Gel Electrophoresis
... acrylamide gel containing a gradient of denaturant that increases in the direction of ...the gel that is sufficiently ...more denaturing and more domains ...the gel where the ...
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Evaluation of Denaturing Gradient Gel Electrophoresis (DGGE) for the Determination of Marine Phytoplankton Genetic Diversity
... doi: http://dx.doi.org/10.13005/bbra/1286 (Received: 10 April 2014; accepted: 20 May 2014) A study was conducted to optimize gradient gel electrophoresis (DGGE) protocols for determination of marine ...
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Microbial profiling of dental caries and periodontitis patients using denaturing gradient gel electrophoresis
... based denaturing gradient gel electrophoresis ...large proportion of Gram positive bacteria, while periodontitis group contained mostly Gram negative ...
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Assessment of the genotypic diversity of antibiotic-producing Pseudomonas species in the rhizosphere by denaturing gradient gel electrophoresis
... samples. Denaturing gra- dient gel electrophoresis (DGGE) of 350-bp fragments of phlD, a key gene involved in DAPG biosynthesis, allowed discrimination between genotypically different phlD ⴙ ...
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Denaturing Gradient Gel Electrophoresis of PCR Amplified gki Genes: a New Technique for Tracking Streptococci
... ent gel electrophoresis (DGGE) technique consisting of spe- cific PCR primers, targeting the household gene glucose kinase (gki), used in combination with DGGE to discriminate be- tween different ...
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Studies of the Diversity of Lactobacillus spp. in Fecal Samples Using PCR and Denaturing Gradient Gel Electrophoresis
... Not all study subject fecal samples were possible to amplify: for the 142 bβ1 sample almost no PCR product was retrieved, and despite two more attempts to receive amplification products from this sample, none of the PCR ...
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Characterization of Microbial Populations in Litopenaeus vannamei (Boone, 1931) Based Biofloc System Using Denaturing Gradient Gel Electrophoresis Profiles of 16s rRNA
... (BFT W ) and clean water as a control without biofloc. The extracted microbial community 16S rRNA fragment of the sample was amplified with GC-clamped F968 and R1378 and evaluated in the DGGE and the distinct bands were ...
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Application of the denaturing gradient gel electrophoresis (DGGE) technique as an efficient diagnostic tool for ciliate communities in soil
... by denaturing gradient gel electrophoresis (DGGE) was developed and used to monitor community shifts in a polycyclic aromatic hydrocarbon (PAH) polluted ...
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Diversity of eukaryotic plankton of aquaculture ponds with Carassius auratus gibelio, using denaturing gradient gel electrophoresis
... in pond 1were significantly higher than pond 2 (p>0.05). The concentration of chlorophyll a (chl a) has no significantly difference between pond 1 and pond 2. DGGE profiles of 18S rRNA gene fragments from the ...
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Distribution of eukaryotic picoplankton assemblages across hydrographic fronts in the Southern Ocean, studied by denaturing gradient gel electrophoresis
... Departament de Biologia Marina i Oceanografia, Institut de Cie`ncies del Mar (CMIMA, CSIC), Passeig Marı´tim de la Barceloneta 37-49, E-08003 Barcelona, Catalonia, Spain Abstract We used a molecular fingerprinting ...
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High Prevalence of Helicobacter Species Detected in Laboratory Mouse Strains by Multiplex PCR Denaturing Gradient Gel Electrophoresis and Pyrosequencing
... Previously, a PCR-DGGE assay targeting the V6-7 region of 16S rDNA was developed for the identification of most Heli- cobacter spp. in the guts of zoo animals, which was based on an optimized Helicobacter genus-specific ...
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Analysis of the bacterial community in chronic obstructive pulmonary disease sputum samples by denaturing gradient gel electrophoresis and real-time PCR
... PCR-denaturing gradient gel electrophoresis (DGGE) with universal primers targeting the V3 region of the 16S rRNA gene was employed to characterize the overall COPD patient sputum microbiota ...
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Denaturing gradient gel electrophoresis analysis of archaeal and bacterial populations in a submerged anaerobic membrane bioreactor treating landfill leachate at low temperatures
... 10°C the methane percentage dropped to 55 % in the SAMBR headspace, while the gas production rate was only 1 and 1.7 L.L -1 .day -1 in SAMBR30 and SAMBR300, respectively. Archaeal population in HR, SAMBR30 and SAMBR300 ...
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Use of 16S Ribosomal DNA PCR and Denaturing Gradient Gel Electrophoresis for Analysis of the Microfloras of Healing and Nonhealing Chronic Venous Leg Ulcers
... and denaturing gradient gel electrophoresis (DGGE) of PCR-amplified 16S rRNA gene ...DGGE profiles also revealed that all of the wounds possessed one apparently common band, identified ...
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Polymerase Chain Reaction Denaturing Gradient Gel Electrophoresis (PCR DGGE) Profile of Bacterial Community from Agricultural Soils in Bauchi, North East Nigeria
... Abstract The population dynamics of bacterial community was investigated in three Agricultural soils, designated as Loamy sand (A), Peaty coarse (B) and Loamy coarse sand (C) in North-East, Nigeria. The soil chemical ...
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