prostaglandin H
Enhanced prostacyclin synthesis in endothelial cells by retrovirus mediated transfer of prostaglandin H synthase cDNA
... A retroviral vector (BAG) was used to transfer human prostaglandin H synthase (PGHS-1) gene into a human endothelial cell line for enhancement of PGI2 synthesis. Cells infected with BAG containing PGHS-1 ...
8
Secretory leukocyte protease inhibitor suppresses the production of monocyte prostaglandin H synthase 2, prostaglandin E2, and matrix metalloproteinases
... of prostaglandin H synthase/cyclooxy- genase (EC ...Two prostaglandin synthase isoforms have been identified, referred to as PGHS-1 and PGHS-2, and are found in many cell types (see review ref- ...
8
A novel electron paramagnetic resonance-based assay for prostaglandin H synthase-1 activity
... to prostaglandin H 2 (PGH 2 ), the pre- cursor of all ...endoperoxide, prostaglandin G 2 (PGG 2 ), and a haem with peroxidase activity that reduces PGG 2 to PGH 2 (For review see ...
7
Hypercalcemia stimulates expression of intrarenal phospholipase A2 and prostaglandin H synthase 2 in rats Role of angiotensin II AT1 receptors
... In chronic hypercalcemia, inhibition of thick ascending limb sodium chloride reabsorption is mediated by elevated intrarenal PGE2. The mechanisms and source of elevated PGE2 in hypercalcemia are not known. We determined ...
11
Lipopolysaccharide induces prostaglandin H synthase 2 protein and mRNA in human alveolar macrophages and blood monocytes
... We and others have previously demonstrated that human alveolar macrophages produce more PGE2 in response to lipopolysaccharide (LPS) than do blood monocytes. We hypothesized that this observation was due to a greater ...
7
Human immunodeficiency virus type 1 envelope protein does not stimulate either prostaglandin formation or the expression of prostaglandin H synthase in THP-1 human monocytes/macrophages.
... This finding agrees with published studies with both alveolar and peritoneal macrophages, which demonstrate extensive prostaglandin biosynthesis. However, despite the capacity to produce prostaglandins, ...
7
Role of intestinal epithelial cells in the host secretory response to infection by invasive bacteria Bacterial entry induces epithelial prostaglandin h synthase 2 expression and prostaglandin E2 and F2alpha production
... Pathogenic bacteria cause diarrhea by multiple mecha- nisms. For example, bacteria such as Vibrio cholerae reside in the lumen of the small intestine and produce toxins that alter ion absorption and/or secretion (6, 7). ...
15
Influence of natural and recombinant interleukin 2 on endothelial cell arachidonate metabolism Induction of de novo synthesis of prostaglandin H synthase
... In an effort to elucidate the mechanism by which IL-2 elicits sustained stimulation of eicosanoid synthesis, we investigated the effects of recombinant r and nIL-2 on endothelial cell AA[r] ...
8
Corticotropin releasing hormone interacts with interleukin 1 to regulate prostaglandin H synthase 2 expression in human myometrium during pregnancy and labor
... Total RNA was extracted from myometrial cell cultures by an RNeasy total RNA kit and reverse transcribed to synthesize cDNA by using RNase H Reverse Transcriptase (Life Technol- ogies). Quantitative PCR was used ...
13
Distinct roles of prostaglandin H synthases 1 and 2 in T cell development
... Prostaglandin G and H synthases, or cyclooxygenases (COXs), catalyze the formation of prostaglandins (PGs). Whereas COX-1 is diffusely expressed in lymphoid cells in embryonic day 15.5 thymus, COX-2 ...
10
Allergic lung responses are increased in prostaglandin H synthase–deficient mice
... volume were calculated for each breath cycle. Compli- ance and resistance were calculated using the constant- flow inflation method single-compartment model (24). All values were averaged over 6-second intervals and ...
13
Differential impact of prostaglandin H synthase 1 knockdown on platelets and parturition
... PGF2α at 8% WT levels was sufficient to induce coordinated temporal oxytocin receptor OTR expression in uterus and normal ovarian luteolysis in PGHS1Neo/Neo mice at late gestation, while[r] ...
11
Effect of ACTH and CRH on Plasma Levels of Cortisol and Prostaglandin F2α Metabolite in Cycling Gilts and Castrated Boars
... vicinity and were expected to come in oestrus within the first week after arrival. After the sec- ond oestrus, the experiments were carried out in the early luteal phase (days 5-10). The gilts were checked twice daily at ...
8
SYNTHESIS, INSILICO DOCKING AND ADMET STUDIES OF ARYL ACETIC ACID DERIVATIVES AS PROSTAGLANDIN ENDOPEROXIDE H SYNTHASE 2 INHIBITORS
... The concentrated alkyl substituted aryl cyanide (0.5-0.6 mol eq) was dissolved in propylene glycol (100 ml) and added potassium hydroxide flakes in lot quantities (exothermic reaction) for 1 hr at below 10°C. The ...
5
Clinical Decision Making of Nurses Regarding Elder Abuse
... phosphate buffer perchloric acid pars distalis of the pituitary gland 3’5’cyclic AMP phosphodiesterase pars distalis removal pelvic ganglia prostaglandins prostaglandin E2 prostaglandin [r] ...
120
Impacts of Cytosolic Phospholipase A2, 15 Prostaglandin Dehydrogenase, and Cyclooxygenase 2 Expressions on Tumor Progression in Colorectal Cancer
... Eighty-nine patients who had undergone surgical resection for primary sporadic colorectal carcinoma by a single colorectal surgeon at the Department of Surgery, Chosun University Hospital between March 2002 and December ...
8
Airway response to acute mechanical stress in a human bronchial model of stretch
... Effect of stretch 5 minutes, 2.5 × basal tone on the production of leukotriene E4 LTE4, prostaglandin screening PG, and prostaglandin E2 PGE2 in an organ bath assessed by ELISA during th[r] ...
11
A fluorescence microscopy method for quantifying the detection of prostaglandin endoperoxide H synthase-1 and CD-41 in MEG-01 cells
... Using DAPI (4’,6-diamidino-2-phenylindole) as a fluorescent nuclear marker, we assessed cell number and specific antibodies to PGHS-1 and CD-41 in order to identify cells expressing thes[r] ...
10
Limited effect of anti rheumatic treatment on 15 prostaglandin dehydrogenase in rheumatoid arthritis synovial tissue
... Canhao H, Edwards CJ, Hamuryudan V, Kvien TK, Leeb BF, Martín-Mola EM, Mielants H, Müller-Ladner U, Murphy G, Østergaard M, Pereira IA, Ramos- Remus C, Valentini G, Zochling J, Dougados M: Multinational ...
9
Altmann, Frank (2007): Differenzielle Expression von Proteinen im Glaskörper gesunder und an ERU erkrankter Pferde. Dissertation, LMU München: Tierärztliche Fakultät
... protein, Prostaglandin-H2 D-isomerase, Dickkopf- related protein 3, Secreted frizzled-related protein 2 and Pigment epithelium-derived factor) were expressed at lower levels in vitreous of horses suffering from ...
122