protein cell-free expression
Cell free expression and in meso crystallisation of an integral membrane kinase for structure determination
... the protein functions with dual ...and cell-free N-terminally hexahistidine-tagged protein. Cell-free expression was similar to that implemented in the current study and ...
32
Cell-free soluble expression of the membrane protein PsbS
... membrane protein with four transmembrane helices and belongs to the light harvesting complex (LHC) protein superfamily (Funk et ...overexpression. Cell-‐free (CF) protein ...
12
Cell-free expression systems for the construction of artificial cells
... of cell-free protein synthesis systems and the exploration of possible ...which protein pools are obtained from engineered strains, yielded a more active cell- free ...
100
The Major Open Reading Frame of the β2.7 Transcript of Human Cytomegalovirus: In Vitro Expression of a Protein Posttranscriptionally Regulated by the 5′ Region
... the expression of ...any expression of a specific ...the cell type ...ORF3 expression resulting from the presence of the 59 leader sequence, confirming the results obtained with the ...
5
A novel methanol-free Pichia pastoris system for recombinant protein expression
... while cell growth was checked by the spotting ...Aox expression was de-repressed in glycerol cultured Δgut1 strain although cell growth was also largely ...
11
A User’s Guide to Cell-Free Protein Synthesis
... to protein yield ...adoption cell-free platforms and their ...adoption cell-free ...(“cell free protein synthesis” OR “in vitro transcription translation” OR “in ...
34
Original Article Establishment of pancreatic cancer stem cells by flow cytometry and their biological characteristics
... cer cell line PC-3 was divided into SP and non-SP cells by flow ...two cell clone spheres and nude mice tumor formation rates were compared by cultivating in serum-free medium; The expression ...
6
Defining the Free Energy Landscape for Protein Induced Cell Membrane Curvature
... from cell-cell ...dictating cell behavior [61, 62]. Conventional cell biology methods study cells plated in monolayers on 2D ...epithelial cell polarity [62–64] and allowing for ...
176
Expression of an endotoxin-free S-layer/allergen fusion protein in gram-positive Bacillus subtilis 1012 for the potential application as vaccines for immunotherapy of atopic allergy
... S-layer protein of Geobacillus stearothermophilus ATCC 12980 or Lysinibacillus sphaericus CCM 2177 resulting in the recombinant fusion proteins rSbsC/Bet v1 or rSbpA/Bet v1, respectively ...T cell epitopes, ...
12
Study of messenger RNA inactivation and protein degradation in an Escherichia coli cell-free expression system
... a cell-free expres- sion system optimized for high protein production ...This cell-free system uses the endogenous ...gene expression in cell-free ...and ...
9
Regulation of sterol carrier protein 2 (SCP2) gene expression in rat peritoneal macrophages during foam cell formation A key role for free cholesterol content
... SCP2 protein and its ...gene expression in macrophages, while incubation with maleylated BSA had no ...gene expression in macrophages. The enhancement of SCP2 gene expression by AcLDL suggests ...
10
Stögbauer, Tobias Roland (2012): Experiment and quantitative modeling of cell-free gene expression dynamics. Dissertation, LMU München: Fakultät für Physik
... fluorescent protein (GFP) inside the ...minimal cell by expressing a second protein, namely the transmembrane protein α-hemolysin in their ...gene expression reaction in the vesicles ...
126
Evaluation of antibody responses to the early transcribed membrane protein family in Plasmodium vivax
... The protein structure and amino acid alignment were carried out using bioinformatics analysis ...germ cell free protein expression system and the purified proteins were used for ...
10
The Hepatitis E Virus Open Reading Frame 3 Product Interacts with Microtubules and Interferes with Their Dynamics
... Hepatitis E virus (HEV) is the causative agent of hepatitis E, a major form of viral hepatitis in developing countries. The open reading frame 3 (ORF3) of HEV encodes a phosphoprotein with a molecular mass of ...
8
Vesicular stomatitis virus matrix protein inhibits host cell-directed transcription of target genes in vivo.
... To determine whether the viral matrix M protein was involved in this inhibition of host cell gene expression, an M protein expression vector was cotransfected with a target gene vector, [r] ...
7
Building Positive Future Orientations: The Role of Natural Mentors in the Lives of African American Girls
... desired cell characteristics when subjected to low serum concentrations (Ades et ...endothelial cell model creates aberrant results using ...B+C cell culture as demonstrated by gross morphology when ...
133
Original Article Clinicopathological significance of PTEN and bcl2 expressions in oral squamous cell carcinoma
... the expression of PTEN of OSCC to inflammatory lesions of oral tissue, the loss of frequency of PTEN expres- sion was statistically significant ...PTEN expression was associated with poor differen- tiation, ...
7
Coexistence of HER2 over expression and p53 protein accumulation is a strong prognostic molecular marker in breast cancer
... The expression of ER-α was scored by assigning a proportion score and an intensity score according to Allred’s procedure ...HER2 expression the membrane staining pattern was estimated and scored on a scale ...
7
Original Article Expression of TDP43 in cervical cancer
... the expression of TDP43 in cervical cancer tissues by immuno- histochemistry ...the expression of TDP43 was increased in cervical cancer tissues compared with normal tissues (Figure ...TDP43 protein ...
7
Expression of prion protein in the cerebrospinal fluid of patients with Parkinson's disease complicated with rapid eye movement sleep behavior disorder.
... Relatively low levels (0.27 ± 0.06) of PrP mRNA were observed in the CSF samples from healthy individuals (Figure 1). The PrP mRNA, however, was significantly elevated in the PD patients without sleeping disorders (1.37 ...
9