protein g- affinity chromatography
Purification of Mycobacterium bovis BCG Tokyo antigens by chromatofocusing, lectin-affinity chromatography, and hydrophobic interaction chromatography.
... (2.778 g/100 ml), ...the protein solutions were stored at 5 8 C with Merthiolate or were frozen at 2 20 8 ...the protein concentra- tion was ...Plus Protein Assay kit (Pierce, Rockford, Ill.) ...
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Inteins and affinity resin substitutes for protein purification and scale up
... pMAL Protein Fusion and Purification System manual ...exchange chromatography step for separating the protease and maltose tag, it was assumed that the same amylose resin would be used twice with ...fusion ...
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Human granulocyte colony stimulating factor (hG-CSF): cloning, overexpression, purification and characterization
... the protein and to allow for full bioactivity recovery in the refolding ...for G-CSF extraction from inclusion bodies produced in ...including G-25, CM-cellulose and G-75 chromato- graphic ...
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Minor Whey Protein Purification Using Ion-Exchange Column Chromatography
... desired protein from other proteins employing ...membrane chromatography (MC) ...column chromatography have been mainly attributed to shorter diffusion times, as the association between target ...
147
Reversible Immobilization of Chelating Affinity Surfactants on Reversed Phase Adsorbents for Protein and Peptide Separations under Metal Affinity Chromatography
... The synthesis of chelating surfactants was performed by a three-step reaction scheme. In step 1, as an example, 10 g of Brij-76 were melted at 55˚C under vacuum to remove water for 1 h. Afterwards 5.2 mL of ...
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Intein Mediated Protein Synthesis and its Purification by Ni- NTA Affinity Chromatography
... The N terminal sequence of the resulted intein was observed to restrict cleavage in its N terminal and allows cleavage only at the C -terminal. The gene contained 4 blocks A, B, F and G for splicing. A and B are ...
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Optical Properties of In1 xGaxN Epilayers Grown by HPCVD
... using affinity (GST-tag) and ion exchange ...6x-his-tag affinity chromatography and the collected fractions were used to grow crystals using the hanging-drop ...
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Expression of the functional recombinant human glycosyltransferase GalNAcT2 in Escherichia coli
... nickel affinity chromatography and was subsequently analysed by size exclusion chromatography coupled to multi-angle light scattering (SEC-MALS) and circular dichroism spectroscopy to determine ...
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Combining metal oxide affinity chromatography (MOAC) and selective mass spectrometry for robust identification of in vivo protein phosphorylation sites
... Protein concentrations were determined via the dye-bind- ing method of Bradford as described previously [45], using ovalbumin as a standard. Each measurement was made in triplicate and the mean values were used. ...
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Purification and Characterization of a 43 Kilodalton Extracellular Serine Proteinase from Cryptococcus neoformans
... ⫻ g for 15 min, and the supernatant was filtered through a ...⫻ g for 30 ...Bound protein was eluted with a stepwise gradient of ...and protein concen- tration and enzyme activity were ...
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Financial Intermediation and Economic Growth: Bank Credit Maturity and Its Determinants
... nickel affinity FPLC ...the affinity FPLC fractions 10-18 and was combined with 10 μL of mixed Laemmli sample ...Plus Protein® molecular weight marker standard (Bio-Rad) was added to lane ...
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Nonstructural proteins of herpes simplex virus. I. Purification of the induced DNA polymerase.
... Thus, DEAE-cellulose and phosphocellulose chromatography were used in combination with affinity chromatography to purify DNA polymerase from herpes simplex virus type 1- and type 2-infec[r] ...
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Single Amino Acid Changes in the Nipah and Hendra Virus Attachment Glycoproteins Distinguish EphrinB2 from EphrinB3 Usage
... Residues near aa 507 in NiV-G are involved in distinct ephrinB3 binding. Since subtle changes at position 507 can significantly impact ephrinB3 receptor usage, our data suggest that residues around this region may ...
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Separation and identification of carp pituitary proteins and glycoproteins
... ABSTRACT: Carp pituitary proteins and glycoproteins were separated by the combination of immobilized metal affinity chromatography (IMAC) and affinity chromatography on Con A-Sepharose. The ...
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Purification of equine infectious anemia virus antigen by affinity chromatography
... Group-specific antigen derived from equine infectious anemia EIA virus has been widely used for the diagnosis of EIA, especially in immunodiffusion tests 1, 6, 9, 10.. However, the antig[r] ...
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SINGLE STEP PURIFICATION OF UREASE FROM CANAVALIA GLADIATA SEEDS
... Urease Assay: Urease activity was quantified by following the release of ammonia from urea by means of modified Berthelot reaction. The enzymatically liberated ammonia was calculated from a previously established ...
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Clinical Decision Making of Nurses Regarding Elder Abuse
... nickel affinity FPLC were combined and placed into three Amicon Ultra 15 filter units with a 5,000 MWCO Ultracel membrane (Millipore, Billerica, ...x g for 15 min at ...remaining protein solution in ...
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Identification of a Linear Heparin Binding Domain for Human Respiratory Syncytial Virus Attachment Glycoprotein G
... thermore, peptide b27 bound only weakly to Vero cells and did not bind to any of the CHO cells assayed, whereas the Vn HBD peptide, also containing an XBBXBX mammalian HBD motif, reacted strongly with both Vero and CHO ...
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DOCKING STUDIES ON SELECTED DENGUE AND RABIES VIRAL STRUCTURAL PROTEINS WITH CORIANDRUM SATIVUM L FLOWER CONSTITUENTS – AN IN SILICO APPROACH
... Dengue virus contains two structural proteins and five non-structural proteins and Rabies virus contains three proteins structural and one non-structural protein, which are considered to be the most effective for ...
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Antimitotic herbicides bind to an unidentifed site on malarial parasite tubulin and block development of liver stage Plasmodium parasites
... Protein affinity assay using the tubulin fusion proteins.. The affinity of the tubulin fusions for other proteins was determined by their ability to.[r] ...
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