protein misfolding
In vitro amplification of H type atypical bovine spongiform encephalopathy by protein misfolding cyclic amplification
... The protein misfolding cyclic amplification (PMCA) method is based on iterative rounds of sonication and incubation to replicate very low levels of PrP Sc seed within PrP C substrate (usually a brain ...
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Protein misfolding in neurodegenerative diseases: implications and strategies
... Chaperones are another possible target class for thera- peutic intervention in protein misfolding disease states. As chaperones are involved in all aspects of proteostasis, they offer potential therapeutic ...
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Highly sensitive detection of small ruminant bovine spongiform encephalopathy within transmissible spongiform encephalopathy mixes by serial protein misfolding cyclic amplification
... It is assumed that sheep and goats consumed the same bovine spongiform encephalopathy (BSE)-contaminated meat and bone meal that was fed to cattle and precipitated the BSE epidemic in the United Kingdom that peaked more ...
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Highly Sensitive Detection of Small Ruminant Bovine Spongiform Encephalopathy within Transmissible Spongiform Encephalopathy Mixes by Serial Protein Misfolding Cyclic Amplification
... It is assumed that sheep and goats consumed the same bovine spongiform encephalopathy (BSE)-contaminated meat and bone meal that was fed to cattle and precipitated the BSE epidemic in the United Kingdom that peaked more ...
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Highly Infectious Prions Generated by a Single Round of Microplate Based Protein Misfolding Cyclic Amplification
... though protein misfolding cyclic amplification (PMCA) has emerged as a valuable, sensitive tool, it is currently hampered by its lack of robustness and rapidity for high-throughput ...
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Proteotoxic stress targeted therapy (PSTT): induction of protein misfolding enhances the antitumor effect of the proteasome inhibitor bortezomib
... of protein misfolding (hyperthermia), inhibition of proteasomes (bortezomib) or inhibition of Hsp90 (geldanamycin) have all been considered or used for cancer ...of protein misfolding ...
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Rapid Profiling of Disease Alleles Using a Tunable Reporter of Protein Misfolding
... In this report, we demonstrate use of IDESA to report subtle changes in steady-state protein levels in cells that relate to stability differences. Importantly, we show that the assay can be fine tuned with ...
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Correlation between Bioassay and Protein Misfolding Cyclic Amplification for Variant Creutzfeldt-Jakob Disease Decontamination Studies
... prion protein observed from the initial human brain materials, the generated Surf-PMCA amplicons obtained from wires contaminated with those brains or from FIG 1 Evaluation of standard and commercially available ...
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Chemical Modification Methods for Protein Misfolding Studies
... Thioamides in Nature and in Medicinal Chemistry In nature, thioamides are found in small molecules and at least one folded protein. To our knowledge, there are five natural products with thioamide bonds, namely ...
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Assessment of the Genetic Susceptibility of Sheep to Scrapie by Protein Misfolding Cyclic Amplification and Comparison with Experimental Scrapie Transmission Studies
... The supernatant was discarded, and 20 l of 1⫻ sample-reducing buffer (Invitrogen) was added to the pellets, heated at 90°C for 10 min, and centri- fuged at 10,000 ⫻ g for 5 min. A total of 15 l of each sample was ...
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De Novo Generation of a Unique Cervid Prion Strain Using Protein Misfolding Cyclic Amplification
... IMPORTANCE CWD is the only known TSE that affects free-ranging wildlife, specifi- cally cervids such as elk, deer, moose, caribou, and reindeer. CWD has become en- demic in both free-ranging and captive herds in North ...
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Versatile biocatalysis of fungal cytochrome P450 monooxygenases
... substantial protein expression and ...non-expression, protein misfolding, or aggregation into insoluble inclusion bodies, as well as slow substrate uptake and lack of compatible reductase systems ...
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Are heat shock proteins therapeutic target for Parkinson's disease?
... aberrant protein aggregation and neuron degeneration are the common pathophysiology of these ...aberrant protein misfolding and inhibit the proapoptotic pathway to attenuate dopaminergic neuron ...
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Misfolding dominates protein evolution
... Protein misfolding is certainly not the only cause of the relationships considered here. But multiple lines of evidence suggest that it may well be the most important determinant of the strength of those ...
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Systems pharmacology of the amyloid cascade : unfolding oligomer modulation in Alzheimer's disease
... of protein aggregates inside or outside of neurons is the leading cause of cellular dysfunction in neurodegenerative ...of protein deposition and the trigger of degenerative signals in the neurons is an ...
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A New Method to Assay Protease Based on Amyloid Misfolding: Application to Prostate Cancer Diagnosis Using a Panel of Proteases Biomarkers
... the misfolding of ...amyloid-beta misfolding on the electrode ...mitigated protein misfolding and evident signal ...the protein misfolding step, high sensi- tivity suitable for ...
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The different axes of the mammalian mitochondrial unfolded protein response
... to protein misfolding and directly causing ROS production are also capable of SIRT3 induction, further emphasizing the role of the sirtuin axis as an antioxidant response, but also indicating SIRT3 levels ...
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Superparamagnetic Nanoparticle Capture of Prions for Amplification
... Prion diseases are associated with the presence of PrP Sc , a disease-associated misfolded conformer of the prion protein. We report that superparamagnetic nanoparticles bind PrP Sc molecules efficiently and ...
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Alpha-Synuclein Misfolding and Aggregation in Parkinson's Disease
... In comparison of PD with the neurodegenerative disorders Alzheimer’s disease (AD), and Amyotrophic Lateral Sclerosis (ALS, Lou Gehrig’s), a common pathology is observed (Lansbury et al., 2003). Upon autopsy, the AD brain ...
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Androgen dependent pathology demonstrates myopathic contribution to the Kennedy disease phenotype in a mouse knock in model
... In Kennedy disease, an expanded glutamine tract near the amino terminus of the androgen receptor AR leads to hormone-dependent protein misfolding and to the predominant loss of lower mot[r] ...
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