Proteoglycan synthesis
Vascular wall proteoglycan synthesis and structure as a target for the prevention of atherosclerosis
... modifying proteoglycan synthesis and structure may represent a prime target to prevent LDL binding and entrapment in the vessel wall and thus prevent the development and progression of ...
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Sequential isolation of proteoglycan synthesis mutants by using herpes simplex virus as a selective agent: evidence for a proteoglycan-independent virus entry pathway.
... in proteoglycan synthesis ...sulfate synthesis, have been shown to be highly refractory to HSV infection ...saminoglycan synthesis unless these molecules were involved in HSV ...
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UDP glucose dehydrogenase modulates proteoglycan synthesis in articular chondrocytes: its possible involvement and regulation in osteoarthritis
... PGs synthesis in human articular ...PGs synthesis of articular chondrocytes, while the suppressed expression of UGDH might probably be involved in advanced OA, partly due to the modulation of p38 MAPK and ...
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Effects of sesamin on chondroitin sulfate proteoglycan synthesis induced by interleukin 1beta in human chondrocytes
... proteoglycan production. This finding is consistent with previous reports. IL-1β induces downregulation of aggrecan and xylosyltransferase-1 expression while it upregulates MMP-13 and ADAMTs expression [14–16, 19, ...
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Extracellular nicotinamide phosphoribosyltransferase (NAMPT/visfatin) inhibits insulin like growth factor 1 signaling and proteoglycan synthesis in human articular chondrocytes
... Studies have shown increased ERK activity in chondro- cytes isolated from osteoarthritic cartilage [30,31]. Inhi- biting ERK/MAPK activation enhanced IGF-1-mediated PG synthesis [24], suggesting that activation of ...
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Short Link N promotes disc repair in a rabbit model of disc degeneration
... stabilizes proteoglycan aggregates in IVD and cartilage by binding to both hyaluronic acid and aggrecan ...enhance proteoglycan synthesis by IVD cells and intact human discs, and also to increase ...
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Differential direct effects of cyclo oxygenase 1/2 inhibition on proteoglycan turnover of human osteoarthritic cartilage: an in vitrostudy
... increase proteoglycan synthesis and diminish proteoglycan release ...on proteoglycan synthesis, retention, release and content in both degenerated (preclinical) and (late-stage) OA ...
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Defective alterations in the collagen network to prostacyclin in COPD lung fibroblasts
... activity. Proteoglycan synthesis was quantified by [ 35 S]-sulfate incorporation into glycosoaminoglycan side-chains measured on a scintillation counter (Wallac; Perkin Ellmer, Boston, MA, ...[22]. ...
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Inhibition of HDAC Activity by ITF2357 Ameliorates Joint Inflammation and Prevents Cartilage and Bone Destruction in Experimental Arthritis
... Inhibition of HDAC activity by ITF2357 modulates several in vivo models of in- flammation, such as LPS- induced shock, Con A–induced hepatitis, DSS colitis and even traumatic brain injury (24,14,25). In these models, it ...
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Potent mitogenic effects of parathyroid hormone (PTH) on embryonic chick and rabbit chondrocytes Differential effects of age on growth, proteoglycan, and cyclic AMP responses of chondrocytes to PTH
... Sulfated proteoglycan synthesis by confluent cultures of rabbit costal chondrocytes grown in the presence of fibroblast growth factor.. Fibroblast growth factor stimulates colony formati[r] ...
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Investigation of the direct effects of salmon calcitonin on human osteoarthritic chondrocytes
... to proteoglycan and collagen type II ...cartilage synthesis to the same level as the positive control, ...investigated proteoglycan synthesis by the gold standard method, radio-labeled ...
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An ultrasound study of altered hydration behaviour of proteoglycan degraded articular cartilage
... In natural OA, changes in water content of articular car- tilage usually are associated with composition and struc- tural alterations of the collagen-PG ECM as well as synovial fluid [3]. Some studies found that GAG loss ...
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Glucosamine reduces the inhibition of proteoglycan metabolism caused by local anaesthetic solution in human articular cartilage: an in vitro study
... Methods: Cartilage explants obtained from 13 femoral heads from fracture neck of femur patients (average age 80 years, 10 female) were exposed to either 1% Lidocaine, 2% Lidocaine, 0.25% Bupivacaine, 0.5% Bupivacaine, ...
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The biological basis of degenerative disc disease: proteomic and biomechanical analysis of the canine intervertebral disc
... evaluate proteoglycan con- tent and distribution throughout the IVD NP and immuno- histochemical methods to verify the differential expression of the proteins identified in our iTRAQ analysis (decorin, biglycan, ...
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TOXICOLOGICAL STUDIES OF THE AQUEOUS LEAF EXTRACT OF CASSIA PETERSIANA BOLLE (CAESALPINIACEAE), AN ANTITYPHOID MEDICINAL PLANT
... of the kidneys are protein and sodium analyses (Ricos et al., 1994). It is clear that two factors are important in excluding proteins at the glomerular level. The first or these is the molecular size, the other one being ...
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The role of aggrecan in normal and osteoarthritic cartilage
... When aggrecan finds itself in an aqueous environment its swells as the sulfated GAG chains become hydrated in an attempt to expand their molecular domain and increase their separation from one another. Within the ECM, ...
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Quantification of Chondrocyte Death and Proteoglycan Content in Mechanically Impacted Articular Cartilage
... The depth-dependent behavior of articular chondrocytes may vary with the presence or absence of subchondral bone damage. Also, fibrillation or cracks in the tissue may also affect the distribution of chondrocyte death ...
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Biomaterial-Mediated Reprogramming of the Wound Interface to Enhance Meniscal Repair
... Growth factors of intermediate efficacy include PDGF-AB and IGF-1 (Imler et al. 2004). PDGF-AB also induces meniscal cell migration (Bhargava et al. 1999) as well as proteoglycan production (Imler et al. 2004, ...
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Metalloproteases of human articular cartilage that digest cartilage proteoglycan at neutral and acid pH
... metalloproteases are described. A neutral protease that digests proteoglycan subunit optimally at pH 7.25 has been purified up to 900-fold. It is strongly inhibited by o- phenanthroline, alpha-2-macroglobulin, and ...
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Identification of the principal proteoglycan binding site in LDL A single point mutation in apo B100 severely affects proteoglycan interaction without affecting LDL receptor binding
... Our results indicate that site B (residues 3359–3369) is the major proteoglycan-binding site in apo-B100. These residues are only present on apo-B100, and not on apo-B48, which is truncated at residue 2153. ...
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