Proteomic analysis by mass spectrometry
Proteomic analysis of endodontic infections by liquid chromatography–tandem mass spectrometry
... defenses. Proteomic analysis of endodontic infections can provide global insights into the invasion, pathogenicity mechanisms, and multifactorial interactions existing between root canal bacteria and the ...
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Removal of SDS from biological protein digests for proteomic analysis by mass spectrometry
... The fundamental step of global proteomics experi- ments, particularly involving sensitive MS technique is efficient sample preparation. Extraction of total proteins from various biological sources including tissues and ...
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Mass Spectrometry-Based Quantitative Proteomic Analysis of Biological Fluids
... current mass spectrometers to deal with all at once despite the recent progress in mass ...MS analysis therefore becomes necessary to increase peak capacity ...the mass spectrometer stays ...
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Quantitative Proteomic Analysis in Candida albicans using SILAC-based Mass Spectrometry
... incorporation efficiency (95.43%) determined from the global analysis of heavy lysine peptides. 3: Arginine to proline conversion. D) A box plot showing a global protein ana[r] ...
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Evaluation of sample preparation methods for mass spectrometry-based proteomic analysis of barley leaves
... After protein digestion, peptides (~ 20 μg) were desalted using Poros ® 20 R2 reversed phase microcolumns as pre- viously described [31]. Dried peptides were dissolved in mobile phase A (0.1% formic acid) and the ...
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Development and Application of Mass Spectrometry Methods for Expression Proteomic Analysis of Marek's Disease Virus Infection.
... resolution mass spectrometers, as well as the development of the required software, now make this task much more feasible ...the mass range analyzed, while others may not be favorably retained on the ...
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Identification of altered protein abundances in cholesteatoma matrix via mass spectrometry-based proteomic analysis
... scale proteomic approach to the identification of these altered protein ...all proteomic studies, although mass spectrometry- based techniques have a wider dynamic range than trad- itional ...
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Proteomic analysis of surface proteins of Trichinella spiralis muscle larvae by two dimensional gel electrophoresis and mass spectrometry
... invasion by the larvae has been developed [5,6], the mecha- nisms by which T. spiralis infective larvae recognize, invade, and migrate within the intestinal epithelium are unknown. Previous studies had showed that the ...
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Comparative proteomic analysis of normal and tumor stromal cells by tissue on chip based mass spectrometry (toc-MS)
... the proteomic changes from nor- mal connective tissue to tumor ...based mass spectrometry ’ ...the proteomic profiles were subse- quently analysed with mass ...into proteomic ...
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MASS SPECTROMETRY-BASED PROTEOMIC ANALYSIS OF COMPLEX BIOLOGICAL SAMPLES PENG ZHAO. (Under the Direction of Lance Wells) ABSTRACT
... cell mass, primitive ectoderm, primordial germ cells, gonadal germ cells and gametes form a developmental life cycle that ensures continued propagation of the ...
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Proteomic profile determination of autosomal aneuploidies by mass spectrometry on amniotic fluids
... broadband proteomic analysis techniques for application to diagnosis of human ...clinical proteomic method, hundreds of samples can be analysed within a short period of time allowing statistical ...
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Proteomic profiling of the mesenteric lymph after hemorrhagic shock: Differential gel electrophoresis and mass spectrometry analysis
... quantitative proteomic study on mesenteric lymph from a well characterized rat shock ...MODS. Mass spectrometry based proteomic analysis of proteins in mesenteric lymph can effectively ...
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Mass Spectrometry Analysis of Cancer Biopsies
... for mass spectrometry-based proteomics, top–down and bottom–up ...of analysis that each group has developed, but they share in common their mode of ...In mass spectrometric analysis, ...
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Proteomic profiling and protein identification by MALDI-TOF mass spectrometry in unsequenced parasitic nematodes.
... tandem mass spectrometry have hampered proteomic investigations into helminths, such as resolving the mechanism underpinning globally reported anthelmintic ...Comparative proteomic ...
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Proteomic profiling and protein identification by MALDI-TOF mass spectrometry in unsequenced parasitic nematodes.
... tandem mass spectrometry have hampered proteomic investigations into helminths, such as resolving the mechanism underpinning globally reported anthelmintic ...Comparative proteomic ...
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Mass Spectrometry-Based Identification of Muscle-Associated and Muscle-Derived Proteomic Biomarkers of Dystrophinopathies
... Degradation of protein biomarkers can occur imme- diately following the collection of biofluids as a result of endogenous or exogenous proteinase activity. To overcome this issue, nanoparticles or nanotraps are able to ...
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Statistical analysis of proteomic mass spectrometry data
... The breast cancer and melanoma datasets were studied in chapter 3 using a Bayesian approach which utilised MCMC algorithms. This approach modelled the spectra as a series of peaks with differing heights and locations. ...
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Data mining in proteomic mass spectrometry
... As the technology involved in biological domains like proteomics advances, large vol- umes of data are continuously generated. However, the data analysis required is increasingly overwhelming to the scientists in ...
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Proteomic profiling of retinoblastoma by high resolution mass spectrometry
... for validation studies on formalin fixed paraffin embed- ded tissue sections. These proteins were overexpressed more than twofold compared to non-neoplastic retina. The staining intensity and distribution is summarized ...
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Classification Using Mass Spectrometry Proteomic Data with Kernel-Based Algorithms
... data analysis that combines logistic regression with kernel matrix, namely, logistic kernel regression (LKR), logistic kernel principal component regression (LKPCR), and logistic kernel partial least square re- ...
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