Proteomic preparation and analysis
Proteomic sample preparation for blast wound characterization
... gel-based proteomic analysis is greatly improved after removing high abundant proteins, but is the same true for wound effluent? Typically wound effluent consists of plasma, red and white blood cells, ...
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Evaluation of sample preparation methods for mass spectrometry-based proteomic analysis of barley leaves
... After protein digestion, peptides (~ 20 μg) were desalted using Poros ® 20 R2 reversed phase microcolumns as pre- viously described [31]. Dried peptides were dissolved in mobile phase A (0.1% formic acid) and the ...
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Proteomic analysis of human vitreous humor
... Proteomic analysis of the vitreous has been carried out by many groups using different techniques as summarized in Table ...sample preparation methods or mass spectrometry platforms ...
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Proteomic Analysis of Merkel Cell Polyomavirus
... 114 5.5.2.5 Compatibility of TCE-based stain-free with western blot. Western blotting is one of the most common techniques used in modern biological research. This method consists of several steps: sample ...
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Datasets: Sensitivity and protein digestion course of proteomic Filter Aided Sample Preparation
... 1. Data 1.1. Sensitivity of filter aided sample preparation The dataset contain mass spectrometry data obtained through analysis of various cell lysate amounts. Aliquots of mouse brain or HeLa sample ...
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Proteomic analysis of the ventral disc of Giardia lamblia
... spectrometry analysis identified several proteins previously known to be part of the ventral disc such as tubulin and ...our analysis could be explained by the lack of relevant hits in some spots of the ...
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Proteomic analysis of wheat α/A and β gliadins
... The strongly exothermic polymerisation reaction required intensive column cooling during the gel preparation. For the separation, 6 ml of the mixture containing the gliadin extract and saturated sucrose solution ...
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A comparative proteomic analysis of bile for biomarkers of cholangiocarcinoma
... sample preparation steps prior to mass spectral analysis and the technology available for performing mass spectrometry have tremen- dous effects on resulting protein profile at both quality and ...
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A quantitative proteomic analysis of long-term memory
... Sample Preparation 0.5 mL of solubilization buffer (Urea-Thiourea-CHAPS buffer containing, 40 mM Tris, 5 M Urea, 2 M thiourea, 4% CHAPS, 10 mM DTT, 1 mM EDTA, 1 mM PMSF, and a Protein inhibitor cocktail) was added ...
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Proteomic Analysis of Oxidative Stress-resistant Cells
... Preparation of RNA and Quantitative RT-PCR—RNAzol was used to prepare RNA. Cells were rinsed with phosphate-buffered saline, lysed on the plate with RNAzol reagent, and prepared as directed. RNA was quantitated ...
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Proteomic analysis of Plasmodium in the mosquito: progress and pitfalls
... would be fascinating to understand whether similar mechanisms also regulate the remaining ‘ latent ’ stage, the hypnozoite. The microgamete. One of the most recent, and currently unpublished, proteomes we have produced ...
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Critical comparison of sample preparation strategies for shotgun proteomic analysis of formalin-fixed, paraffin-embedded samples: insights from liver tissue
... sample preparation strategy can provide qualitatively and quantitatively different proteomic infor- mation, and that each method presents typical advantages and drawbacks that should be taken into account ...
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Critical comparison of sample preparation strategies for shotgun proteomic analysis of formalin-fixed, paraffin-embedded samples: insights from liver tissue
... sample preparation procedures based on a protein extraction step (such as FASP and ISD) may imply a depletion of high-MW proteins compared to methods founded on a whole tissue ...
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Proteomic and transcriptomic analysis of the Protozoan Parasite Neospora caninum
... This analysis has utilized the powerful technique of gel separation followed by liquid chromatography and mass spectrometry to confidently identify almost 20 % of the ...one proteomic platform, as the vast ...
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Proteomic analysis of Chilo iridescent virus
... first proteomic analysis of an invertebrate iridovirus, 46 viral proteins were detected in the virions of Chilo iridescent virus (CIV) based on the detection of 2 or more distinct peptides; an additional 8 ...
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Proteomic analysis of the secretome of Leishmania donovani
... LC-MS/MS analysis of leishmania ...spectrometry analysis was designed to compute ratios of simultaneously detected spectra from mixed Cm and CA sam- ...
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Proteomic analysis of the Clostridium thermocellum cellulosome
... cellulosome protein complex using nanoLC-ESI-MS peptide sequencing; and, second, to quantitatively assess changes in the subunit profiles within cellulosomes isolated from cells grown [r] ...
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Proteomic analysis of the EhV-86 virion
... functional analysis of the gene products. Essential to the functional analysis is the identification of the proteins associated with the vir- ion ...
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Proteomic analysis of the Theileria annulata schizont
... a b s t r a c t The apicomplexan parasite, Theileria annulata, is the causative agent of tropical theileriosis, a devastating lymphoproliferative disease of cattle. The schizont stage transforms bovine leukocytes and ...
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Proteomic analysis of the chondrocyte cell surface
... In this project, the shotgun method was applied as the major procedure. As a complementary procedure, nearly all the obvious gel bands were cut off. According to my identification results, although some proteins are ...
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