Proteomic studies on maturing human dendritic cells
Molecular signatures of maturing dendritic cells: implications for testing the quality of dendritic cell therapies
... Although monocytes, iDCs, and mDCs have been characterized at a molecular level, few studies have com- prehensively studied the molecular events associated with DC maturation. In this study we compared the ...
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Functional studies on human γδ T cells and their interactions with dendritic cells and B cells
... 51 2.5 Flow cytometry 2.5.1 Principles of flow cytometry Flow cytometry is a powerful tool for the analysis of multiple parameters of individual cells within heterogeneous populations. The cell sample is injected ...
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Analysis of proteomic profiles and functional properties of human peripheral blood myeloid dendritic cells, monocyte derived dendritic cells and the dendritic cell like KG 1 cells reveals distinct characteristics
... KG-1 cells, our findings iden- tified important differences between the proteomes of these cells, and the findings were confirmed by ELISA detection of a selection of ...the cells, again suggesting ...
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Histamine regulates cytokine production in maturing dendritic cells, resulting in altered T cell polarization
... Previous studies have shown that IL-10 blocks IL-12 expression by inhibiting NF- κ B activa- tion (52, 53); how the histamine/H2 receptor, IL-10, and LPS/TLR4 pathways interact during the instruc- tional phase of ...
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Interaction of Rotavirus with Human Myeloid Dendritic Cells
... T cells that secrete gamma interferon circulate in recently infected and seropositive adults and ...mature dendritic cells (MDC) in ...these cells expressed ...T cells stimulated by ...
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Human Immunodeficiency Virus Fusion to Dendritic Cells Declines as Cells Mature
... Immunoblotting studies revealed that 81A virions did not incorporate more gp41 or BlaM-Vpr than NL4-3 virions ...of cells supporting fusion in a dose-dependent manner ...
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Quantitative proteomic changes in LPS activated monocyte derived dendritic cells : a SWATH MS study
... In conclusion, this study further supports SWATH-MS as a robust technology for the quantitative study of proteins involved in cellular processes, such as is demonstrated here for pathogenic stimulation of moDCs. The data ...
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Proteomic profile of human monocytic cells infected with dengue virus
... U937 cells infected with the DENV-2/NG ...other studies showing that the percentage of infected monocytes in vitro are much lower compared with the percentages obtained from epithelial, hepatic, and ...
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Proteomic analysis of exosomes derived from human lymphoma cells
... all cells in all life forms, but can also be released into the extracellular environment in the absence of cellular necrosis ...viable cells have not yet been eluci- dated, but we propose that exosomes play ...
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Proteomic analysis of human periodontal ligament cells under hypoxia
... for degradation [27, 28]. HSPA8 extensively interacted with proteins including HSP beta 1 (HSPB1) and heat shock 70 kDa protein 5 (HSPA5), synergistically exerting anti-apoptotic effects on hPDLCs. Additionally, HSPs ...
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Proteomic profiling of endorepellin angiostatic activity on human endothelial cells
... Notably, the α2β1 integrin interacts with vimentin and co-localizes with the integrin in endothelial cell focal adhesions and contributes to the vimentin-associated matrix adhesion structures identified in endothelial ...
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Comparative study of clinical grade human tolerogenic dendritic cells
... Conclusions In summary, in these comparative analyses of clinical grade tol-DCs, Dexa-and VitD3-DCs exhibited a “ semi- immature ” phenotype and IL-10 secretion. In contrast, Rapa-DCs induced CD4+CD25 hi CD127 low FoxP3+ ...
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Human Immunodeficiency Virus Type 1 Activates Plasmacytoid Dendritic Cells and Concomitantly Induces the Bystander Maturation of Myeloid Dendritic Cells
... mucosal cells targeted during oral and sexual trans- mission (9, 16, 31, ...T cells (26, ...few studies have questioned whether pDCs routinely accumulate in mucosal ...
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Human Plasmacytoid Dendritic Cells Sense Lymphocytic Choriomeningitis Virus-Infected Cells In Vitro
... G2 cells transfected with cloned hepatitis B virus ...1953. Studies on the propagation in vitro of poliomyelitis ...of human malignant epithelial cells (strain HeLa) derived from an epider- ...
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Role of DC-SIGN in Lassa Virus Entry into Human Dendritic Cells
... previous studies, we found that many human cell types targeted in vivo by LASV, such as epithelial cells, vascular endothelial cells, and hepatocytes, express functionally glycosylated DG able ...
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Interactions between dengue type 3 viruses and human dendritic cells
... Department of Microbiology and Immunology, 1 Department of Genetics, 2 The Carolina Vaccine Institute, 3 University of North Carolina-Chapel Hill, Chapel Hill NC. 4.1 Abstract Much of the experimental work on dengue ...
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Nanostructured lipid carriers loaded with resveratrol modulate human dendritic cells
... Previous studies have shown the activity of rsv to counteract proinflammatory stimuli in a variety of ...most studies, rsv was added directly to culture media, lead- ing to high doses being necessary for ...
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Variability in the response of canine and human dendritic cells stimulated with Brucella canis
... In this study, peripheral monocytes from healthy donors were purified and then differentiated into DCs to analyze the effects of whole B. canis or its purified LPS and to compare the production of pro-inflammatory ...
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Human dendritic cells in blood and airways during respiratory viral infection
... vitro studies indicate that in the lungs, hantavirus may result in infection of lung endothelial cells, epithelial cells or immune cells present in the lungs such as alveolar macrophages or ...
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Phagocytosis of Picornavirus-Infected Cells Induces an RNA-Dependent Antiviral State in Human Dendritic Cells
... mouse studies, while still little is known regarding the role of these viral sensors in human ...of human monocyte-derived DCs with echovirus (EV), but not coxsackie B virus (CVB), results in rapid ...
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