Protoplast Fusion
Protoplast cultures and protoplast fusion focused on Brassicaceae: A review
... are protoplast isolations and protoplast fusions, in particular their history, a review of factors influencing the protoplasts isolation and fusion, selection of hybrid plants and utilization of ...
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Effects of Protoplast Fusion on δ-endotoxin Production in Bacillus thuringiensis Spp. (H14)
... Protoplast fusion is a versatile technique for inducing genetic recombination in a variety of prokaryotic and eukaryotic microorganisms, such as actinomycetes and Bacillus ...
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Improving the nematicidal potential of Bacillus amyloliquefaciens and Lysinibacillus sphaericus against the root-knot nematode Meloidogyne incognita using protoplast fusion technique
... were 70.85, 84.69, 95.56, 94.99, 100, and 89.46% due to the parental strains B. amyloliquefaciens and L. sphaericus and the fusants Bas3, Bas6-2, Bas8, and Bas11, respectively. There was a positive correlation between ...
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Molecular Genetic Characterization of Fusants from Protoplast Fusion of S. Cerevisiae and P. Stipitis ATCC 58785
... from protoplast fusion between ...intrageneric protoplast fusion between Aspergillus oryzae and Trichoderma harzianum, protein content of the fusant was 60 µg/mL while those of the parental ...
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Introduction of mitochondrial DNA from Pleurotus ostreatus into Pleurotus pulmonarius by interspecific protoplast fusion
... chloramphenicol-resistance gene, suggesting that the P. os- treatus mtDNA had been introduced into P. pulmonarius cells by interspecifi c protoplast fusion. Considering the ex- perimental conditions, defi ...
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Enhancement of Xylanase Production by Protoplast Fusion of Trichoderma spp.
... of protoplast fusion between different species as a successful strategy to generate enzyme-super producing strains of filamentous fungi lacking inherent sexual ...
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Protoplast fusion for bloat-safe and nutritionally improved lucerne
... Protoplast fusion allows the transfer of blocks of ...a protoplast regeneration protocol has to be worked out and the genes are introduced at random and with imprecision as in conventional ...of ...
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Adenovirus type 12 E1A protein expressed in Escherichia coli is functional upon transfer by microinjection or protoplast fusion into mammalian cells.
... 420-427 0022-538X/86/080420-08$02.00/0 Copyright C 1986, American Society for Microbiology Adenovirus Type 12 ElA Protein Expressed in Escherichia coli Is Functional upon Transfer by Mic[r] ...
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Noncomplementing Diploids From Bacillus subtilis Protoplast Fusion: Relationship Between Maintenance of Chromosomal Inactivation and Segregation Capacity
... and segregation analysis of strains bearing polymorphic NotI restriction sites (ITAYA and TANAKA 1991) to obtain further evidence of the genetic and physical structure of Ncd[r] ...
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EFFECT OF RADIATION DOSAGE ON EFFICIENCY OF CHLOROPLAST TRANSFER BY PROTOPLAST FUSION IN NICOTIANA
... The protoplasts of the organelle donor were irradiated with different lethal doses using a WO source, to facilitate the elimination of their nuclei from the fusion prod[r] ...
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CYCLOSPORIN A PRODUCTION BY GENETICALLY IMPROVED FUSANTS OF TOLYPOCLADIUM INFLATUM
... fermentation. Protoplast fusion had been applied to increase the productivity of ...intra-specific protoplast fusion between them was ...
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Understanding protoplast technology as a tool to enhance biocontrol
... or the RAPD pattern differ from one or the other parental strain. Moreover, in all progeny the mitochondrial genotype matched the same pattern of the nuclear genotype with similarities to a single parent opposed to a ...
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IMPROVEMENT OF FIELD CROPS THROUGH BIOTECHNOLOGY METHODS
... and indica rices (Yamada, et al., 1985; Abdullah, et al., 1986; Kyozuka, et al., 1987; Lee et al., 1989). Hayashi, et al., (1988) obtained somatic hybrids between O. sativa and O. officinalis, O. elchingeri, O. ...
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Optimization Conditions of Wheat Mesophyll Protoplast Isolation
... plant protoplast could be traced back to the 1960s that the British bo- tanist Cocking used enzyme degrading cell wall to extract protoplast of apical tomato ...then, protoplast research was at the ...
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Preparation and regeneration of protoplast from antitumor agent epothilone producing microbes myxobacteria
... the protoplast can’t regenerate, it will lead to be lack of materials for experiment to select, and it is very difficult to ...of protoplast for epothilone-producing myxobacteia, mainly due to differences ...
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Genome Shuffling of Pseudomonas Sp Ioca11 for Improving Degradation of Polycyclic Aromatic Hydrocarbons
... fused protoplast preparation was diluted with regeneration media (MSM containing ...The protoplast were re-suspended in regeneration media and shaken at 200 rpm for 12 h before plating on MSM agar plates ...
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Karyotype analysis of interspecific fusants of basidiomycetes by pulsed-field gel electrophoresis
... some authors 6-9 performed intraspecific protoplast fusion and others 8'1~ interspecific protoplast fusion of ed- ible mushrooms.. the method using a chemical fusogen has[r] ...
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Interaction between Geminivirus Replication Protein and the SUMO-Conjugating Enzyme Is Required for Viral Infection
... of the infiltrated leaf, which is indicative of AL1-AL1 interac- tions (Fig. 3A). We also observed YFP fluorescence mostly in nuclei of cells coinfiltrated with constructs corresponding to NYFP-AL1 and CYFP-NbSCE1 (Fig. ...
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Dovzhenko, Alexander (2001): Towards plastid transformation in rapeseed (Brassica napus L.) and sugarbeet (Beta vulgaris L.). Dissertation, LMU München: Fakultät für Biologie
... of protoplast derived colonies was observed in our experiments as ...Nevertheless, protoplast derived colonies, large enough for transfer to solid medium, were formed only 6-7 weeks after protoplast ...
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Purification and functional characterization of protoplasts and intact vacuoles from grape cells
... The viability of the protoplasts was tested with the fluorescent dye fluorescein diacetate (FDA). The intact plasma membrane is permeable to FDA, and FDA is converted into a green fluorescent dye, fluorescein, by ...
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