Proviral load
Quantification of HTLV I proviral load in experimentally infected rabbits
... that proviral load studies may be conducted with little concern for effects of mutations on primer recognition of the ...provirus. Proviral load in PBMC of HTLV-I infected asymptomatic ...
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Correlation between LTR point mutations and proviral load levels among Human T cell Lymphotropic Virus type 1 (HTLV 1) asymptomatic carriers
... PvL: Proviral load; LTR: Long terminal repeat; ATL: Adult T-cell leukemia; HAM/TSP: HTLV-I-associated myelopathy/tropical spastic paraparesis; CREB: Transcriptional activity of cAMP binding protein; NFkB: ...
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Single nucleotide polymorphisms in the bovine MHC region of Japanese Black cattle are associated with bovine leukemia virus proviral load
... Studies of BLV-associated host factors identified poly- morphisms within the bovine major histocompatibility complex (MHC) (BoLA) [21–29]. Recently, Miyasaka et al. revealed that polymorphisms within BoLA class II ...
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Gag-Specific CD4+ T-Cell Frequency Is Inversely Correlated with Proviral Load and Directly Correlated with Immune Activation in Infection with Human Immunodeficiency Virus Type 2 (HIV-2) but Not HIV-1
... Human immunodeficiency virus type 2 (HIV-2) infection, unlike HIV-1 infection, is normally characterized by low rates of CD4 depletion and low-to-undetectable viremia. We found that the frequency of Gag-specific CD4 ⴙ T ...
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Quantification of Human Immunodeficiency Virus Type 1 Proviral Load by a TaqMan Real Time PCR Assay
... Specificity and HIV-1 subtypes amplified. The primers and the probe were chosen to quantify HIV-1 group M subtype B, the most frequent subtype found in the European population, representing 80 to 85% of the strains ...
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New Insights into Prevalence, Genetic Diversity, and Proviral Load of Human T Cell Leukemia Virus Types 1 and 2 in Pregnant Women in Gabon in Equatorial Central Africa
... Human T-cell leukemia virus type 1 (HTLV-1) is highly endemic in areas of central Africa; mother-to-child transmission and sexual transmission are considered to be the predominant routes. To determine the prevalence and ...
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Human T lymphotropic virus type 1 (HTLV 1) proviral load induces activation of T lymphocytes in asymptomatic carriers
... viral load is posi- tively correlated with the intensity of T-cell activation, as well as the destruction of CD4 + T-lymphocytes and a progression to AIDS ...HTLV-1 proviral load (PVL) is not widely ...
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Human T lymphotropic virus type 1 (HTLV 1) prevalence and quantitative detection of DNA proviral load in individuals with indeterminate/positive serological results
... To date, blood screening for HTLV-I has not been manda- tory in Italy, but a more careful screening of the popula- tion might be justified by several literature reports [22- 24]. Screening tests are usually based on ...
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Development and Validation of a Multiplex Real Time PCR Assay for Simultaneous Genotyping and Human T Lymphotropic Virus Type 1, 2, and 3 Proviral Load Determination
... ventional serological techniques such as enzyme-linked immu- nosorbent assay and Western blotting. However, among sam- ples infected with HTLV-1 or HTLV-2, the proportion of seroindeterminate results is high (20, 21, 28, ...
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Bovine leukemia virus proviral load is more strongly associated with bovine major histocompatibility complex class II DRB3 polymorphism than with DQA1 polymorphism in Holstein cow in Japan
... diagnosis, proviral load is a major diagnosis index for the determination of disease progression and transmission ...BLV proviral load in the Holstein ...high proviral load and ...
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In vivo expression of the HBZ gene of HTLV 1 correlates with proviral load, inflammatory markers and disease severity in HTLV 1 associated myelopathy/tropical spastic paraparesis (HAM/TSP)
... HTLV-1 proviral load [36] and tax mRNA expression [17] in PBMCs or HTLV-1 infected cell lines as reported previ- ...HTLV-1 proviral load was calcu- lated using β-actin as an internal control ...
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Increased proviral load in HTLV 1 infected patients with rheumatoid arthritis or connective tissue disease
... drome, two with inflammatory myopathy, and one with systemic lupus), who were seropositive for HTLV-1, were included in the study. Samples were collected between September 2001 and June 2002. For one patient, sequen- ...
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BLV CoCoMo qPCR: Quantitation of bovine leukemia virus proviral load using the CoCoMo algorithm
... The BLV-CoCoMo-qPCR method was also accurate. The provirus copy number obtained using real-time PCR with CoCoMo primers correlated closely with the result from serial dilution nested PCR. Because we aimed to use ...
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Quantification of Proviral Load of Human Immunodeficiency Virus Type 2 Subtypes A and B Using Real Time PCR
... high proviral loads have been reported (13, 20, 34), normal prolif- eration of infected cells could explain the large number of PBMC harboring proviral ...high proviral loads and inefficient ...
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Lack of evidence to support the association of a single IL28B genotype SNP rs12979860 with the HTLV 1 clinical outcomes and proviral load
... In this study, we sought to replicate specific findings of association between the polymorphism rs12979860 in the vicinity of the IL28 gene and the HTLV-1 PvL, which is itself a predictive factor for development of HAM/ ...
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HTLV 1 drives vigorous clonal expansion of infected CD8+ T cells in natural infection
... high proviral load (PVL; the number of proviral copies per 100 cells) in peripheral blood mononuclear cells (PBMCs) is cor- related with the risk of both the central nervous system inflammatory ...
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Mannose receptor may be involved in small ruminant lentivirus pathogenesis
... with proviral load, as observed in MR ...gland proviral load was increased either in animals with moderate lesions in this organ compared to those with mild lesions (p= ...lung proviral ...
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Rapid dissemination of human T-lymphotropic virus type 1 during primary infection in transplant recipients
... he liver and both kidneys were retrieved from a deceased female Caucasian donor, who was not known to carry, and had no risk factors for, HTLV-1 infection. he organs were transplanted in accordance with UK Blood and ...
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Immunotherapy with internally inactivated virus loaded dendritic cells boosts cellular immunity but does not affect feline immunodeficiency virus infection course
... To determine the effect of immunotherapy on FIV infec- tion, the viral burden in the peripheral blood of experi- mental cats was measured at the time intervals shown in Figure 1 up to 15 weeks after the initiation of ...
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Human immunodeficiency virus type 1 (HIV 1) proviral DNA load in purified CD4+ cells by LightCycler® Real time PCR
... DNA proviral load in PBMC may therefore lead to undetectable proviral ...DNA load in CD4+ cells and PBMCs could be useful as the CD4 isolation adds extra work ...relative proviral ...
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