Pulse-chase
Feeding Preferences of Abyssal Macrofauna Inferred from In Situ Pulse Chase Experiments
... previous pulse chase experiments at Station M ...previous pulse chase studies at abyssal sites and demonstrating that surface deposit feeding community is relatively more important in the ...
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Short term fate of intertidal microphytobenthos carbon under enhanced nutrient availability: a 13C pulse chase experiment
... initial pulse of nutrients was added to both in- cubation tanks and bags holding replacement water for sam- pling, 1 h prior to cores being transferred into the incubation ...additional pulse of NH + 4 was ...
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Posttranslational modifications of Sindbis virus glycoproteins: electrophoretic analysis of pulse-chase-labeled infected cells.
... 42, 1982 TRANSPORT OF PLASMA MEMBRANE GLYCOPROTEINS version step was no longer detectable: however, the 53K to 50K E1 form conversion was still clearly detectable after acid precipitatio[r] ...
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Intramembrane protease RasP boosts protein production in Bacillus
... As a first approach to assess the possible function of RasP in protein secretion under fermentation-mimick- ing conditions, the rasP gene was deleted from the B. subtilis genome and the secretion of three representa- ...
9
Kinetic Analysis of Human Immunodeficiency Virus Type 1 Assembly Reveals the Presence of Sequential Intermediates
... of pulse-chase analysis and biochemical techniques allowed us to temporally order these intermediates and to characterize their sizes and intracellular ...
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Chromogranin A processing and secretion: specific role of endogenous and exogenous prohormone convertases in the regulated secretory pathway
... [35S]Methionine/cysteine pulse-chase metabolic labeling in AtT-20 antisense PC1 and antisense furin cells revealed reciprocal changes in secreted chromogranin A COOH-terminal fragments i[r] ...
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Validation of a Medium-Throughput Method for Evaluation of Intracellular Growth of Mycobacterium tuberculosis
... Luminometry-based versus viable-count-based assessment of intracellular mycobacterial growth. hMDMs from different donors were infected with H37Rv-lux in several independent experiments (n ⫽ 6) at an MOI of 10 using a ...
5
The c terminal extension of a hybrid immunoglobulin A/G heavy chain is responsible for its Golgi mediated sorting to the vacuole
... The work presented so far shows that the secretory pathway in plants expressing IgA/G is not overloaded and that the IgA/G tetrameric molecule is transported to the vacuole via the Golgi complex where it is subjected to ...
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Evidence of ambiguous processing and selective degradation in the noncapsid proteins of rhinovirus 1A.
... Medium P5 is 903 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest Pulse-chase kinetics and extensive pactamycin mapping studies show that the translation of rhinovirus 1[r] ...
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Bacteriophage T4 Head Morphogenesis III. Some Novel Properties of Gene 13-Defective Heads
... DISCUSSION Pulse-chase, temperature-shift experiments suggested that 300S, gene 13-defective unfilled heads isolated from infected cultures served as phage precursors.. However, sections[r] ...
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Simultaneous synthesis and degradation of rat liver glycogen An in vivo nuclear magnetic resonance spectroscopic study
... Keeping in mind that the labeling technique used in the pulse-chase experiment only reports on the turnover of the glycogen labeled during the D-[l-3C]glucose infusion period, it seems r[r] ...
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Intracellular distribution of Sindbis virus membrane proteins in BHK-21 cells infected with wild-type virus and maturation-defective mutants.
... Pulse-chase experiments with wild-type strain SVHR virus-infected cells showed that virus envelope proteins were incorporated initially into membranes of the rough endoplasmic reticulum [r] ...
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Intracellular synthesis of mouse mammary tumor virus polypeptides: indication of a precursor glycoprotein.
... For pulse-chase experiments, cell on whole cell cytoplasmic extracts from mouse cultures were pulsed for 15 min with 2 ml of labeling mammary tumor cells, the major MTV glyco- medium con[r] ...
9
Metabolism of cerebroside sulfate and subcellular distribution of its metabolites in cultured skin fibroblasts from controls, metachromatic leukodystrophy, and globoid cell leukodystrophy
... With pulse-chase study of 1-[14C]stearic acid-labeled cerebroside sulfate (14C-CS) and subsequent subcellular fractionation by Percoll gradient, the metabolism of CS and translocation of its metabolites in ...
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Biosynthetic Properties of a Polyoma Nucleoprotein Complex: Evidence for Replication Sites
... In the pulse-chase experiments, the whether the py DNA in the 55S complex can radioactive medium was removed by aspiration, the 32 Downloaded from http://jvi.asm.org/ on November 10, 201[r] ...
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Mobilferrin is an intermediate in iron transport between transferrin and hemoglobin in K562 cells
... Separation of membrane components. K562 cells were selected for these studies because they possess both the transferrin–transferrin re- ceptor and the MIP pathway to transport iron into the cell (21). Fur- ther, they ...
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Rapid intracellular turnover of adenovirus 5 early region 1A proteins.
... We report here an investigation of the turnover of the ElA proteins with immune precipitation with specific antiserum in pulse-chase labelings of productively infected HeLa cells and in [r] ...
5
Synthesis of Sindbis virus nonstructural polypeptides in chicken embryo fibroblasts.
... 22, 1977 SINDBIS VIRUS NONSTRUCTURAL POLYPEPTIDES 421 in Sindbis virus-infected cells and show by tryptic peptide mapping and pulse-chase experiments that these polypeptides are synthesi[r] ...
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Expression of human immunodeficiency virus type 1 (HIV-1) gag, pol, and env proteins from chimeric HIV-1-poliovirus minireplicons.
... A pulse-chase analysis of the protein expression from the chimeric genomes demonstrated the initial rapid proteolytic processing of the polyprotein from the chimeric genomes to give HIV-[r] ...
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The gene order for rubella virus structural proteins is NH2-C-E2-E1-COOH.
... By employing the widely used method of synchronizing initiation of protein synthesis by treatment of infected cells with hypertonic salt 14 in conjunction with pulse-chase labeling, immu[r] ...
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