qRT-PCR
Combination of conventional immunohistochemistry and qRT-PCR to detect ALK rearrangement
... a qRT-PCR assay (Amoy Diag- nostics, Xiamen, China) approved by European Con- formity (CE marking) and the China Food and Drug Administration (CFDA), was applied on formalin-fixed paraffin embedded (FFPE) ...
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Selection of reference genes for qRT PCR examination of wild populations of Atlantic cod Gadus morhua
... liver ARP and RPL37 were ranked as the two best genes, with Ubi ranking number three (Fig. 1C). ACTB and ARP- 2, two genes encoding structural proteins, were ranked as the most unsuitable reference genes in liver. Two ...
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Evaluating L1CAM expression in human endometrial cancer using qRT-PCR
... using qRT-PCR on fresh frozen tissue ...Although qRT-PCR enables an objective evaluation of L1CAM mRNA levels, this method however, has the disadvantage not to reflect the protein level of ...
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Analytical and clinical performance of a Chikungunya qRT PCR for Central and South America
... on qRT-PCR, but available assays have a number of limitations. A qRT-PCR assay specific to the chikungunya E1 gene was designed using sequence data from contemporary ...chikungunya ...
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Detection of 22 common leukemic fusion genes using a single-step multiplex qRT-PCR-based assay
... This advanced screening method allowed us to detect gene fusions in 53.7% of leukemia patients. In agreement with previous reports, we detected fusion genes in 69.4% patients with AML, and 33.3% with ALL [2, 4]. ...
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Evaluation of the effects of tropical tanniferous plants on rumen microbiota using qRT PCR and DGGE analysis
... for qRT-PCR and DGGE were extracted from the in vitro contents at t ½ and 24 h incuba- tion, following the recommendations described in Yu and Foster ...
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Myostatin and IGF I signaling in end stage human heart failure: a qRT PCR study
... comprehensive qRT-PCR analysis of Mstn and IGF-I signaling was carried out by measuring expression of Mstn, its receptor Activin receptor IIB (ActRIIB), IGF-I, IGF-I receptor (IGF-IR), and the negative ...
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Selection and evaluation of reference genes for qRT-PCR analysis in Euscaphis konishii Hayata based on transcriptome data
... housekeeping genes reported in model plant species due to the lack of genetic and sequence genome information [6]. Consequently, the unsuitable use of traditional house- keeping genes as reference gene in non-model ...
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Original Article Upregulation of HOXA13 as a potential tumorigenesis and progression promoter of LUSC based on qRT-PCR and bioinformatics
... et al. proposed three me- chanisms that drive the de- regulation of aberrant HOX genes expression, including temporospatial deregulation, gene dominance and epigen- etic deregulation. The first two mechanisms were dem- ...
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Original Article Spatiotemporal expression of Wnt5a during the development of the striated muscle complex in rats with anorectal malformations
... real-time PCR (qRT-PCR) between normal rat embryos and embryos with ...and qRT-PCR, the higher expression of Wnt5a protein and mRNA were remarkable in normal embryos during the SMC ...
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lncRNA FOXD2-AS1 promotes hemangioma progression through the miR-324-3p/PDRG1 pathway
... assay, qRT-PCR, and rescue experiments, we found FOXD2-AS1 could regulate the malignancy behaviors of HA cells via targeting the miR-324-3p and PDRG1 ...
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Original Article Differential expressions of BMPR1α, ACTN4α and FABP7 in Hirschsprung disease
... stenotic colon segment tissue than that in the normal colon segment tissue in the HSCR patients (Figure 1B). In the mucous layer and muscular layer the quantity of ACTN4α immu- noreactivity showed a regional increase in ...
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MicroRNA-130a alleviates human coronary artery endothelial cell injury and inflammatory responses by targeting PTEN via activating PI3K/Akt/eNOS signaling pathway
... Our study aims to investigate the roles of microRNA-130a (miR-130a) in human coronary artery endothelial cells (HCAECs) injury and inflammatory responses by targeting PTEN through the PI3K/Akt/eNOS signaling pathway. ...
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Decreased expression of cell proliferation-related genes in clonally derived skin fibroblasts from children with Silver-Russell syndrome is independent of the degree of 11p15 ICR1 hypomethylation
... The data are presented as the mean and standard devi- ation. The data from the qRT-PCR, protein, and prolifera- tion assays were compared using the two-sided unpaired t test, and the microarray data were ...
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Dysregulation of miR-15a and miR-214 in human pancreatic cancer
... To investigate the potential functions of miR-15a and miR-214 in pancreatic cancer, we first measured the via- bility of cells transfected with miR-15a/miR-214 mimics or their controls (mimics-NC) using the CCK-8 assay. ...
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Angiogenesis and vasculogenic mimicry are inhibited by 8-Br-cAMP through activation of the cAMP/PKA pathway in colorectal cancer
... control. qRT-PCR and Western blot demonstrated that 8-Br-cAMP significantly enhanced PKA expression, corresponding to the results of the IHC analy- sis on in vivo tumors treated with 8-Br-cAMP ...
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Characterization and function analysis of a novel gene, Hc maoc 1, in the parasitic nematode Haemonochus contortus
... transcription PCR (qRT-PCR) with specific primers was carried out to determine the relative abundance of Hc-maoc-1 transcripts in all key stages ...by qRT-PCR (25 μl) using SYBR® Green ...
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Hepatitis C Virus Infection Modulates Expression of Interferon Stimulatory Gene IFITM1 by Upregulating miR-130A
... IFITM1 mRNA levels using similar conditions. Quantitative re- verse transcription-PCR (qRT-PCR) data revealed that Huh7 cells transfected with anti-miR-130a display enhanced expression of IFITM1 mRNA ...
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Therapeutic Potential of Small Molecule MicroRNA Inhibition.
... CCTTTCATCCAAAGGCAAAACTACAC-3'. qRT-PCR experiments were performed to determine target mRNA ...levels. qRT-PCR was conducted by BioRad MyiQ RT-PCR thermal cycler using the BioRad iScript ...
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Microvesicles secreted from equine amniotic-derived cells and their potential role in reducing inflammation in endometrial cells in an in-vitro model
... Receptor; qRT-PCR: Quantitative reverse transcription polymerase chain reaction; RT-PCR: Reverse transcription polymerase chain reaction; SV: Shedding vesicle; TEM: Transmission electron microscopy; ...
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