Quantitative Reverse Transcriptase Polymerase chain Reaction (qRT-PCR)
Heminested reverse transcriptase polymerase chain reaction (hnRT PCR) as a tool for rabies virus detection in stored and decomposed samples
... disease. Reverse-Transcriptase Polymerase Chain Reaction (RT-PCR) has been used in rabies diagnosis with good results, even in decomposed ...
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A comparative analysis of routine techniques: Reverse transcriptase polymerase chain reaction (RT-PCR) and five cell lines for detection of enteroviruses in stool specimens
... Background and objectives: Each year, Enteroviruses infect millions of people and cause different diseases. The agents are usually detected using cell culture. RD (Rhabdomyosarcoma) and L20B (L cells) are among the ...
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Her2 assessment using quantitative reverse transcriptase polymerase chain reaction reliably identifies Her2 overexpression without amplification in breast cancer cases
... 44. Cuadros M, Talavera P, Lopez FJ, Garcia-Perez I, Blanco A, Concha A. Real- time RT-PCR analysis for evaluating the Her2/neu status in breast cancer. Pathobiology. 2010;77:38–45. 45. Baehner FL, Achacoso N, ...
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Detection of carcinoembryonic antigen messenger RNA in blood using quantitative real time reverse transcriptase polymerase chain reaction to predict recurrence of gastric adenocarcinoma
... M: Quantitative detection of disseminated free cancer cells in peritoneal washes with real-time reverse transcriptase- polymerase chain reaction: a sensitive predictor of outcome ...
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Ex vivo screening for immunodominant viral epitopes by quantitative real time polymerase chain reaction (qRT PCR)
... by quantitative real-time PCR ...by qRT-PCR has also been used to determine the cytokine profile of tumor microenvironment [9] or to monitor cancer-specific immune responses ...by ...
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Expression analysis of three isoforms of hyaluronan synthase and hyaluronidase in the synovium of knees in osteoarthritis and rheumatoid arthritis by quantitative real time reverse transcriptase polymerase chain reaction
... pairs, 200 nmol/L concentration of the corresponding probe, and 2.5 µL Pre-Developed TaqMan assay reagents of endogenous control human β-actin, which contained β- actin-specific primers and probes labeled with a ...
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Improved methods using the reverse transcriptase polymerase chain reaction to detect tumour cells
... efficiency limits the amount of RNA and consequently blood volume that may be analysed in any single PCR. Amplification of poly-A + RNA isolated from whole blood total RNA was more sensitive than analysis of total ...
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Detection of Norwalk virus in stool specimens by reverse transcriptase polymerase chain reaction and nonradioactive oligoprobes
... For agarose gels, 21 coded stool specimens were purified by Sephadex G-200 spin-column chromatography, amplified by RT-PCR by using Norwalk virus NV-5'Pol and NV-3'Pol primers (left lane[r] ...
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Application of in situ reverse trancriptase-polymerase chain reaction (RT-PCR) to tissue microarrays
... were less prone to detachment during processing, so these were chosen for the in situ RT-PCR experiments. Whilst the standard de-waxing step was carried out to remove the embedding material, the extensive ...
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An analysis of the subtypes of dengue fever infections in Barbados 2003–2007 by reverse transcriptase polymerase chain reaction
... Conclusion In conclusion, it would therefore be expected that with the predominance of DENV-3 circulating in the population, although this infection may not be severe as previous when DENV-2 predominated, there will be a ...
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Canine Distemper Virus detection based in Hemaglutinine Gene as target in Reverse Transcriptase-Polymerase Chain Reaction
... The cases of CD are a major concern within the task veterinary clinic. Thus, in recent years, the appearance of many cases has alarmed the veterinary doctors, since they have a special characteristic, the appearance of ...
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Rapid detection and typing of dengue viruses from clinical samples by using reverse transcriptase polymerase chain reaction
... As previously stated, the RNA isolated from these infected mosquitoes was originally found negative for dengue type 2 virus by the RT-PCR assay at all time points. However, when the RNA [r] ...
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Diagnosis of tubercular uveitis by quantitative polymerase chain reaction
... the reaction mixtures of 25 μl were 600 nM for each forward and reverse ...a PCR template. A total of five standards prepared by cloning PCR amplified product corresponding to MPB64 gene of ...
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RT-PCR Fact Sheet Real Time Reverse Transcription Polymerase Chain Reaction (Real Time RT-PCR) Test.
... Reverse transcription PCR, or RT-PCR. This allows the use of RNA as a template. This allows the detection and amplification of RNA. The RNA is reverse transcribed into complementary DNA, using ...
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Detection of Rhinovirus, Respiratory Syncytial Virus, and Coronavirus Infections in Acute Otitis Media by Reverse Transcriptase Polymerase Chain Reaction
... DISCUSSION In the present study we found that common respi- ratory viruses, specifically HRV, RSV, and HCV, were detectable by RT-PCR in 48% of MEF samples and in 75% of children with AOM. These results confirm ...
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Deteksi Dan Identifikasi Chrysanthemum Stunt Viroid Pada Tanaman Krisan Menggunakan Teknik Reverse Transcriptase Polymerase Chain Reaction (Detection and Identification of Chrysanthemum Stunt Viroid on Chrysanthemum Using Reverse Transcriptase Polymerase
... ABSTRAK. Chrysanthemum stunt viroid (CSVd) merupakan salah satu viroid yang menginfeksi tanaman krisan di Indonesia. Tujuan penelitian ialah untuk mengembangkan metode deteksi CSVd secara molekuler dan mengkarakterisasi ...
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Reference genes for quantitative reverse transcription polymerase chain reaction expression studies in wild and cultivated peanut
... biotic and abiotic stresses. For that, we used the three most commonly used statistical programs, GeNorm, NormFinder, and BestKeeper. It is the first in-depth study of reference genes validation in wild Arachis spe- cies ...
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Reverse Transcriptase Pcr Protocol Pdf
... is reverse transcriptase pcr protocol ...initial pcr? This is often accomplished by comparison to a standard curve generated using known genome equivalents or nucleic acid harvested from a ...
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A duplex real time reverse transcriptase polymerase chain reaction assay for detecting western equine and eastern equine encephalitis viruses
... Then we constructed and optimized the duplex real-time RT-PCR assay by adjusting the concentrations of primers and probes. Using a series of dilutions of transcripts containing target genes as template, we showed ...
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Quantitative determination, by real time reverse transcription polymerase chain reaction, of aromatase mRNA in invasive ductal carcinoma of the breast
... Real-time reverse transcription polymerase chain reaction PCR was performed using a ...The PCR reac- tion was carried out in a 20 µ l final volume containing the following: H 2 O ...
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