rabbit reticulocyte lysate
Alphacoronavirus Transmissible Gastroenteritis Virus nsp1 Protein Suppresses Protein Translation in Mammalian Cells and in Cell-Free HeLa Cell Extracts but Not in Rabbit Reticulocyte Lysate
... in rabbit reticulocyte lysate (RRL; Promega) for 10 min (A) or HeLa S10 extract for 30 min (B) in the presence of 1 g of purified GST, TGEV nsp1 (TGEV), SCoV nsp1 (SCoV), or SCoV nsp1-mt (SCoVmt), ...
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Removal of the genome-linked protein of foot-and-mouth disease virus by rabbit reticulocyte lysate.
... Although it has been shown that the migration of S fragment of FMDV RNA is affected by treatment of the RNA with a rabbit reticulocyte lysate, it is not clear whether this treatment brea[r] ...
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Elevated adenosine deaminase activity and hereditary hemolytic anemia Evidence for abnormal translational control of protein synthesis
... Translation of proband poly A+ reticulocyte mRNA in a rabbit reticulocyte lysate system and immunoprecipitation of 35S-labeled protein products with anti-ADA antibody yielded a band of a[r] ...
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A translation strategy and gene map for pea seedborne mosaic virus
... Translation of potyvirus RNA in a rabbit reticulocyte lysate: identification of nuclear inclusion proteins of tobacco etch virus RNA translation and cylindrical inclusion protein as a pr[r] ...
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Evidence for translation of the Borna disease virus G protein by leaky ribosomal scanning and ribosomal reinitiation.
... using G-50 desalting columns (5 Prime33 Prime Inc.), and 500 mg of each cRNA was translated with a rabbit reticulocyte lysate (Promega) in a 50-ml reaction. Each translation reaction was split into ...
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Cell type-specific proteins which interact with the 5' nontranslated region of hepatitis A virus RNA.
... Label transfer to ribosomal salt wash RSW proteins prepared from HeLa cells A and rabbit reticulocyte lysate RRL B after UV cross-linking to RNA probes representing different segments of[r] ...
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Cell-free translation of murine coronavirus RNA.
... The six murine hepatitis virus-specific subgenomic RNAs were partially purified by agarose gel electrophoresis and translated in an mRNA-dependent rabbit reticulocyte lysate, and the cel[r] ...
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The E1∧E4 Protein of Human Papillomavirus Type 16 Associates with a Putative RNA Helicase through Sequences in Its C Terminus
... The region of E4-DBP involved in the association with E1∧E4 in yeast was expressed from pET28a using a rabbit reticulocyte lysate and shown to bind MBP.16 E1∧E4 immobilized on amylose re[r] ...
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Maturation of giardiavirus capsid protein involves posttranslational proteolytic processing by a cysteine protease.
... the rabbit reticulocyte ...from rabbit reticulocyte lysate or Sf9 cells for proper folding of the viral precursor protein to attain proteolytic ...
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Expression of Bottom Component RNA of Cowpea Mosaic Virus in Cowpea Protoplasts
... Comparison of the peptide patterns of the virusinduced proteins with those of the cowpea mosaic virus RNA-coded polypeptides produced in rabbit reticulocyte lysate showed that the 170- a[r] ...
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La autoantigen enhances and corrects aberrant translation of poliovirus RNA in reticulocyte lysate.
... These studies stem from the initial observation that translation of poliovirus RNA in a rabbit reticulocyte lysate is very inefficient and produces aberrant polypeptides, but translation[r] ...
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Action of erythropoietin in vitro on rabbit reticulocyte membrane Ca2+ ATPase activity
... on reticulocyte Ca2+-ATPase required an incubation time of 1 h before enzyme assay for maximum effect and was neutralized by antierythropoietin ...from rabbit mature red blood cells was not inhibited by ...
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Translation initiation in GB viruses A and C: evidence for internal ribosome entry and implications for genome organization.
... in rabbit reticulocyte lysates in vitro ...in reticulocyte lysates reflects a requirement for a spe- cific host cell translation factor which is absent in reticulocyte ...in ...
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Gene protein products of SA11 simian rotavirus genome.
... DISCUSSION the of SA1l proteins synthesized in Analysis infected cells, or in rabbit reticulocyte lysates upon addition of the denatured dsRNA viral segments, has allowed us to identify [r] ...
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Encephalomyocarditis virus 3C protease: efficient cell-free expression from clones which link viral 5' noncoding sequences to the P3 region.
... Translation of encephalomyocarditis virus EMC RNA in rabbit reticulocyte extracts produces active viral peptides, including protease 3C, which is responsible for many cleavage reactions [r] ...
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Translation of Southern Bean Mosaic Virus RNA in Wheat Embryo and Rabbit Reticulocyte Extracts
... The nuclease-treated lysate was dialyzed for 2 h against a solution containing 10 mM HEPES N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid-potassium hydroxide pH 7.6, 1 mM mag51 Down[r] ...
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cis- and trans-cleavage activities of poliovirus 2A protease expressed in Escherichia coli.
... A portion of the poliovirus polyprotein containing the 2APrO cleavage site at the P1/P2 junction was produced by translation of cDNA transcripts in rabbit reticulocyte lysates and then t[r] ...
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Synthesis of Ribonucleic Acid in Rabbit Blood Cells In Vivo
... RNA was isolated from 1 the total population of washed cells, which includes reticulocytes and leukocytes; 2 shock lysates, which represent primarily reticulocyte lysate; and 3 the sedim[r] ...
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Evidence that vesicular stomatitis virus produces double-stranded RNA that inhibits protein synthesis in a reticulocyte lysate.
... Effect of melting and micrococcal nuclease on the residual fractional capacity of polyA+ RNA from VSV-infected HeLa cells and polyI:polyC to inhibit protein synthesis by reticulocyte lys[r] ...
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A rabbit reticulocyte model for the role of hemin controlled repressor in hypochromic anemias
... inhibit reticulocyte protein ...the reticulocyte cell-free systems, plus and minus hemin, premature appearance of HCR was found in cells incubated in vitro with alpha,alpha-dipyridyl or ...
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