Random amplification of polymorphic DNA (RAPD) analysis
Concordance of clinical and environmental isolates of Cryptococcus neoformans var gattii by random amplification of polymorphic DNA analysis and PCR fingerprinting
... by random amplification of polymorphic DNA (RAPD), using 12- to 22-mer primers in pairs, and/or PCR fingerprinting with a single primer derived from the microsatellite core sequence of the ...
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Comparative typing of Pseudomonas aeruginosa by random amplification of polymorphic DNA or pulsed field gel electrophoresis of DNA macrorestriction fragments
... by random amplification of polymorphic DNA (RAPD) and pulsed-field gel electrophoresis (PFGE) of macrorestriction ...identified DNA polymorphisms beyond epidemiologically and ...
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An analysis of apricot cultivars by random amplified polymorphic DNA and microsatellite primers
... Both techniques may provide useful information on the level of polymorphism and diversity in ap- ricot. Repeating twice each amplification reaction and using conservative criteria of band selection can lead to ...
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Molecular typing by random amplification of polymorphic DNA and M13 southern hybridization of related paired isolates of Aspergillus fumigatus
... quality DNA of large molecular ...Taq DNA polymerase and reaction buffer, can have a significant effect on the ...in DNA concentration over a 20-fold range from ...Taq DNA polymerase were ...
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Random amplified polymorphic DNA (RAPD) markers and its applications
... of random sequence bind several priming sites on the complementary se- quences in the template genomic DNA and produce discrete DNA products if these prim- ing sites are within an amplifiable ...
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In vitro culture of Gypsophila paniculata L. and random amplified polymorphic DNA analysis of the propagated plants
... the random amplified polymorphic DNA (RAPD) ...of amplification products generated by polymerase chain reaction was 142 bands ...The analysis of bands recorded, showed ...the DNA ...
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Authentication and Genetic Origin of Medicinal Angelica acutiloba Using Random Amplified Polymorphic DNA Analysis
... 10-mer random primers (OPA, OPB, OPC, and OPD series) to investigate whether they detected ...all DNA templates isolated from the three varie- ties, and 4 gave highly reproducible polymorphic RAPD ...
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Analysis of Salmonella enterica Serovar Typhi by Outer Membrane Protein (OMP) Profiling, Random Amplification of Polymorphic DNA (RAPD) and Pulsed Field Gel Electrophoresis (PFGE)
... Profiling, Random Amplification of Polymorphic DNA (RAPD) and Pulsed Field Gel Electrophoresis (PFGE)] for typing of Salmonella enterica serovar ...
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Random amplification of polymorphic DNA reveals serotype specific clonal clusters among enterotoxigenic Escherichia coli strains isolated from humans
... Based on the present results, we suggest that the use of two primers, one able to identify the major clonal clusters (such as primer 1254 or 1252) and another able to better disclose in- traserotype variations (such as ...
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Typing and Subtyping of 83 Clinical Isolates Purified from Surgically Implanted Silicone Feeding Tubes by Random Amplified Polymorphic DNA Amplification
... RAPD analysis sup- ported all three findings stated so far: that multiple sources contribute to biofilm composition, proliferation of a specific organism occurs, and patient cross acquisition is ...
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RANDOM AMPLIFIED POLYMORPHIC DNA ANALYSIS TO DIFFERENTIATE STRAINS OF VIBRIO VULNIFICUS ISOLATED FROM COCKLES AND SHRIMPS.
... provide polymorphic band ...in amplification of genomic DNA of Vibrio vulnificus generating fragments of DNA ranging in sizes between ...the amplification patterns of the two primers ...
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GENETIC DIVERSITY AND RANDOM AMPLIFIED POLYMORPHIC DNA ANALYSIS OF PESTALOTIA SP ISOLATES OF ENDOPHYTES FROM DIFFERENT HOST
... Fusarium oxysporumf. sp. vasinfectum of worldwide origin. Based on pathogenicity testes on five differential cotton cultivars and species, isolates were differentiated in to three races (A, 3 and 4), restricted to ...
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Random Amplification of Polymorphic DNA and Microsatellite Genotyping of Pre and Posttreatment Isolates ofCandida spp from Human Immunodeficiency Virus Infected Patients on Different Fluconazole Regimens
... Received 31 December 1997/Returned for modification 12 March 1998/Accepted 26 May 1998 Twelve patients infected with the human immunodeficiency virus (HIV) and with CD4 cell counts below 100 cells/ m l received ...
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Genetic Diversity of Finger Millet [Eleusine coracana (L.) Gaertn] Landraces Characterized by Random Amplified Polymorphic DNA Analysis
... (PCR) amplification and electrophoresis RAPD primers were screened based on their ability to detect distinct and clearly resolved amplified ...PCR amplification and electrophoresis were undertaken to ...
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Determination of Genetic Diversity of Four Guppy Strains Using the Random Amplified Polymorphic DNA-PCR
... ABSTRACT This research aims to determine genetic diversity of four strains guppy, respectively are japan blue double sword (JBD), japan blue tiger double sword (JBTD), blue moscow (BM), and panda guppy (PG) with RAPD-PCR ...
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Genetic diversity of maize (Zea mays) accessions revealed by random amplifi ed polymorphic DNA markers
... RAPD analysis of callus regenerated and seed grown plants of maize (Zea mays L.). Rev Bras Milho e Sorgo 2008;7:93-104. El Kichaoui A, Abu Zayed MA, Ayesh BM. Genotyping and identification of six date palm ...
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Experimental embryonic somatic chimaerism in the sheep confirmed by random amplified polymorphic DNA assay
... for DNA analysis. The random amplified polymorphic DNA-PCR method supplied two markers of chimae- rism, which were amplification products of 643 bp and 615 bp long DNA ...
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Rapid discrimination of Mycobacterium tuberculosis strains by random amplified polymorphic DNA analysis
... Mixed-linker polymerase chain reaction: a new method for rapid fingerprinting of isolates of the Mycobacterium tuberculosis complex. Insertion element IS986 from Mycobacterium tuberculos[r] ...
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Application of random amplified polymorphic DNA analysis to differentiate strains of Salmonella enteritidis
... As seen in Table 1, seven phage types are found among the collection of 29 S. enteritidis isolates. However, molecular sub- typing methods reveal that the collection is significantly more genetically diverse than is ...
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GENOTYPING AND IDENTIFICATION OF SIX DATE PALM (PHOENIX DACTYLIFERA L.) CULTIVARSOF THE GAZA STRIP BY RANDOM AMPLIFICATION OF POLYMORPHIC DNA
... cultivars. DNA extraction DNA was extracted from 100 mg new tender white to pale yellow leaves to increase the DNA yield, and to avoid high levels of endogenous phenolics, polysaccharides, or other ...
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