Real-time PCR assays
Real Time PCR Assays for Detection of Bocavirus in Human Specimens
... develop real-time PCR assays for HBoV that would be suitable for diagnostic appli- ...The assays performed equiv- alently, with a reproducible detection limit of 10 target genome copies ...
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Comparative Evaluation of Real Time PCR Assays for Detection of the Human Metapneumovirus
... of real-time RT-PCR assays performed in a LightCycler instrument and designed to amplify the viral nucleoprotein (N), matrix (M), fusion (F), phosphoprotein (P), and polymerase (L) ...the ...
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Comparison of Real Time PCR Assays with Fluorescent Antibody Assays for Diagnosis of Respiratory Virus Infections in Children
... (13), real-time PCR requires less hands-on time per specimen than FA, which is ...labor-intensive. Real-time PCR assays offer advantages over conventional ...
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Performances of Four Real Time PCR Assays for Diagnosis of Pneumocystis jirovecii Pneumonia
... available PCR assays have been ...four real-time PCR assays, including three commercial kits, AmpliSens, MycAssay, and Bio-Evolution PCR, and an in-house PCR ...
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Development of Conventional and Real Time PCR Assays for Detection of Legionella DNA in Respiratory Specimens
... a PCR assay based on the use of new 16S ribosomal DNA (rDNA)- targeted primers to detect Legionella DNA in respiratory specimens are ...conventional PCR followed by electrophoretic detection and was then ...
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Real Time PCR Assays To Address Genetic Diversity among Strains of Mycoplasma hyopneumoniae
... Numerous PCR assays for the detection of ...hyopneumoniae PCR assays could result in false-negative results in current PCR ...hyopneumoniae-specific PCR assays. Some M. ...
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Comparison of Serological and Real Time PCR Assays To Diagnose Bordetella pertussis Infection in 2007
... sensitive real-time PCR assays and single-serum pertussis serology have been developed to overcome these limitations, but there are few data available on the relative sensitivities and ...
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Real Time PCR Assays of Single Nucleotide Polymorphisms Defining the Major Brucella Clades
... clade, assays were tested against a large and diverse collection of 338 iso- ...the assays successfully amplify each allele with minimal cross-hybridization of probes, SNP-based real-time ...
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Clinical Validation of Multiplex Real Time PCR Assays for Detection of Bacterial Meningitis Pathogens
... laboratory assays are critical for effective public health ...Singleplex real-time PCR assays have been developed to detect ...multiplex assays to reduce time and cost. ...
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Inhibition Controls for Qualitative Real Time PCR Assays: Are They Necessary for All Specimen Matrices?
... for real-time PCR anal- ysis across a variety of analytes in 28 qualitative real-time PCR assays using the LightCycler ...These assays consisted of 28 qualitative ...
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Comparison of the FilmArray Respiratory Panel and Prodesse Real Time PCR Assays for Detection of Respiratory Pathogens
... In conclusion, the results of the study indicate that the pre- market version of the FilmArray RP has diagnostic perfor- mance generally comparable to that of the FDA-cleared panel of Prodesse real-time ...
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Development of a panel of seven duplex real-time PCR assays for detecting 13 streptococcal superantigens
... ventional PCR system was at most a 10 -2 ...duplex real-time PCR assays for the detection of SpeA, SpeC, SpeG and SpeM were ob- served to be lower by one log unit compared with that ...
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Development of real-time PCR assays for the quantitative detection of herpesviruses
... I. INTRODUCTION Kaposi’s sarcoma-associated herpesvirus (KSHV) or human herpesvirus 8 (HHV- 8) and Epstein-Barr virus (EBV) or human herpesvirus 4 (HHV-4) are both members of the viral subfamily of gamma herpesviruses. ...
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Development of Real Time PCR Assays for Genotyping of Chlamydia trachomatis
... nested real-time PCR (NRT-PCR) for the genotyping of Chlamydia trachomatis and used it specifically for the typing of either eight genovars from D to K or three genovars of lymphogranuloma ...
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Predicting the sensitivity and specificity of published real-time PCR assays
... A freely available real time PCR analysis tool called TaqSim [4] was used to find public sequences that would match the primer/probe assay in question. TaqSim uses BLAST searches to find sequences ...
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Comparison and Recommendations for Use of Dientamoeba fragilis Real Time PCR Assays
... the real-time PCR platform used, true prevalence rates for nations using the laboratory- developed real-time assay may be approximately 9% lower than reported unless specific cutoff ...
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Comparison of Commercial Real Time PCR Assays for Quantification of Epstein Barr Virus DNA
... quantitative assays for EBV based on real-time PCR: the RealArt EBV LC PCR kit and the LightCycler EBV quantification ...nested PCR samples were used as negative ...nested ...
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Detection of Cryptococcus neoformans DNA in Tissue Samples by Nested and Real-Time PCR Assays
... Two PCR protocols targeting the 18S rRNA gene of Cryptococcus neoformans were established, compared, and evaluated in murine cryptococcal ...nested PCR to be performed in conventional block thermal ...
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Development of multiplex real time PCR assays for identification of members of the Anopheles funestus species group
... new assays. The MCA and HRM assays initially gave promising results, but were more sensitive to both DNA quality and quantity and consequently showed a higher rate of incorrect ...
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Comparative evaluation of published real time PCR assays for the detection of malaria following MIQE guidelines
... first time, the performances of several PCR as- says developed by different laboratories for detection of malaria have been compared side by ...qPCR assays, data was generated with clearly de- fined ...
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