Real time PCR steps using TaqMan chemistry
Detection of ovine milk adulteration using taqman real-time pcr assay
... principle TaqMan real-time PCR systems have been tested for the identification and quantification of bovine DNA in ovine milk ...extracted using the InnuPREP DNA Mini Kit and quantified ...
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A TaqMan real time PCR assay for the detection and quantitation of Plasmodium knowlesi
... A real-time PCR assay for ...the TaqMan hydrolysis probe, Plasprobe, recog- nizes all species of Plasmodium that infect humans, including ...search using the Pk probe sequence did not ...
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Protocol. Introduction to TaqMan and SYBR Green Chemistries for Real-Time PCR
... Green PCR Master Mix is covered by ...for using only this amount of product for the purchaser’s own internal ...for real-time PCR) and no right to perform commercial services of any ...
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Real-time PCR TaqMan assay for rapid screening of bloodstream infection
... conventional PCR assays incorporate a pair of oligo- nucleotide primers to amplify a specific target gene that is then detected using agarose gel electrophoresis combined with an intercalating dye ...The ...
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Rapid detection of porcine circovirus type 2 using a TaqMan based real time PCR
... different real-time PCR system, which amplified a 149-bp fragment to detect ...The real- time PCR approach increased the detection of PCV2 samples by 18% over that achieved by ...
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Original Article Development of TaqMan real-time PCR for detection of stenotrophomonas maltophilia
... Test for specificity Genomic DNA of all the bacterial strains was extracted according to the method mentioned below. The purified cells were cultured in LB or TSB broth that containing 5% bovine serum and 0.002% (w/v) ...
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Quantification of avian influenza virus in tissues of mute swans using TaqMan real time qRT PCR
... and TaqMan probe ...accomplished using a standard curve generated from ten-fold serial dilutions of recombinant plasmid DNA in the range of 10 2 to 10 8 ...
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TaqMan Multiplex PCR Optimization For optimization of Multiplex PCR using 7500/7500 Fast, ViiA 7, and QuantStudio Real-Time PCR Systems
... fluids, infectious agents, and blood of humans and other animals have the potential to transmit infectious diseases. Follow all applicable local, state/provincial, and/or national regulations. Wear appropriate protective ...
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Detection and Genotyping of Varicella Zoster Virus by TaqMan Allelic Discrimination Real Time PCR
... TaqMan PCR does not require postamplification steps, as the formation of PCR product is monitored by real-time mea- surement of reporter dye fluorescence, and thus, there is no ...
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Novel Method for Genotyping Hepatitis B Virus on the Basis of TaqMan Real Time PCR
... Moreover, real-time PCR has the general advantage of tech- nical simplicity without post-PCR steps and a low risk of false results due to ...
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Identification of the main malaria vectors in the Anopheles gambiae species complex using a TaqMan real time PCR assay
... new TaqMan addresses this requirement and has application as a vec- tor/non-vector identification test for large parts of sub- Saharan ...previous TaqMan assay this method will also correctly identify a ...
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Investigating the presence of aspergillus fumigatus and A. Flavus using galactomannan enzyme assay and taqman real-time PCR technique
... PCR, real-time PCR can be beyond beneficial due to its quantitative evidence on the fungal burden, which can dis- tinguish infection from colonization ...(31). TaqMan real- ...
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Identification of the main malaria vectors in the Anopheles gambiae species complex using a TaqMan real-time PCR assay
... on PCR ampli- fication of ribosomal DNA (rDNA) sequences ...multiplex PCR method using species specific primers to amplify products which are of a diagnostic size when vis- ualized by agarose gel ...
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Multilocus Sequence Typing of Mycoplasma hyorhinis Strains Identified by a Real Time TaqMan PCR Assay
... by using the different microorgan- isms listed in Table 1 as DNA ...the PCR test was 537 fg per assay or 107 fg per l (mean cycle threshold [C T ] ⫽ ...
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A TaqMan Real-time PCR System for the Identification and Quantification of Bovine DNA in Meats, Milks and Cheeses
... by PCR amplification of DNA is based on the presence of mamma- lian somatic cells in the milk ( Herman, 2001 ...plex PCR procedures have been developed for species identification in milk, cheese and yogurt ...
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Quantitative detection of cariogenic Streptococcus mutans from saliva using TaqMan assay by Real-Time PCR: A case study in Allahabad
... designed TaqMan probe based Geno-Sen’s SM Kit for quantitative detection of Streptococcus mutans using Real-Time PCR model Rotor Gene 6000 (Corbett Research, Australia) on the basis of ...
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Original Article The impact of storage time on HCV RNA quantitative detection in serum samples by TaqMan real-time PCR
... 14-day period at 4°C in the high- and intermedi- ate HCV RNA load groups and no significant decrease in the HBV DNA titer was detected. However, for the samples stored for 21 and 28 days, the mean viral loads differed ...
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Evaluation of Polymerase Chain Reaction and Cobas TaqMan Real Time PCR in the Diagnosis of Tuberculosis: Indian Prospective
... of PCR in the diagnosis of extra pulmonary tuberculosis cases has been evaluated extensively as an alternative diagnostic tool and has yielded variable results, with sensitivities ranging between 42 and 100% and ...
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Development of TaqMan® MGB fluorescent real time PCR assay for the detection of anatid herpesvirus 1
... established real-time PCR assay to evaluate the sensi- tivity of the system and the detection limit was found to be ...conventional PCR and the established FQ-PCR using dilution ...
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Quantification of Human Immunodeficiency Virus Type 1 Proviral Load by a TaqMan Real Time PCR Assay
... DISCUSSION TaqMan real-time PCR allows simultaneous amplification and quantification, thereby eliminating the need for further manipulation of PCR ...competitive PCR, whose range ...
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