Recombinant adeno-associated virus
Dynamin Is Required for Recombinant Adeno-Associated Virus Type 2 Infection
... Recombinant adeno-associated virus (rAAV) vectors for gene therapy of inherited disorders have demon- strated considerable potential for molecular ...a recombinant adenovirus expressing ...
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Recombinant Adeno-Associated Virus Utilizes Cell-Specific Infectious Entry Mechanisms
... of recombinant adeno-associated virus (rAAV) into host cells can lead to evolution in capsid and vector design and delivery methods, resulting in enhanced transduction and therapeutic gene ...
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Phenotypic correction of Fanconi anemia in human hematopoietic cells with a recombinant adeno associated virus vector
... We demonstrated efficient transduction, expression, and phenotypic correction in lymphoblastoid cell lines derived from FA C patients using a recombinant adeno-associated virus rAAV vect[r] ...
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Genetic Fate of Recombinant Adeno-Associated Virus Vector Genomes in Muscle
... Recombinant adeno-associated virus (rAAV) vectors are promising human gene transfer vectors, because they mediate long-term gene expression in ...
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Immunological Aspects of Recombinant Adeno-Associated Virus Delivery to the Mammalian Brain
... In the human, antigen-specific immunity has been studied in healthy subjects and cystic fibrosis patients, revealing that al- most all had antibodies to AAV serotype 2 (AAV2) derived from naturally acquired infections, ...
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Recruitment of wild-type and recombinant adeno-associated virus into adenovirus replication centers.
... AAV nucleic acids and proteins localize to adenovirus rep- licative domains in coinfected cells. We used in situ DNA hybridization to visualize the nucleic acid of replicating AAV in the nuclei of cells coinfected with ...
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Novel Strategy for Generation and Titration of Recombinant Adeno-Associated Virus Vectors
... the virus to transduce cultured ...binant virus genomes or the expression of an easily detectable transgene is measured (1, 20, ...helper virus used (15), the choice of promoter and cell type used ...
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Kinetics of Recombinant Adeno-Associated Virus-Mediated Gene Transfer
... Adeno-associated virus type 2 (AAV-2) is a nonpathogenic (10, 11), ...DNA virus that requires coin- fection with helper adenovirus or herpes simplex virus for efficient replication (5, ...
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SARS CORONAVIRUS: AN OVERVIEW
... of recombinant adeno- associated virus encoding receptor-binding domain of severe acute respiratory syndrome coronavirus (SARS-CoV) spike protein induces strong mucosal immune responses and ...
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Adeno associated virus gene delivery of broadly neutralizing antibodies as prevention and therapy against HIV 1
... using recombinant adeno-associated virus (rAAV) is a promising alternative to conventional vaccines for preventing new HIV-1 infections and for therapeutically suppressing established HIV-1 ...
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Adeno-associated virus type 2-mediated transfer of ecotropic retrovirus receptor cDNA allows ecotropic retroviral transduction of established and primary human cells.
... a recombinant adeno-associated virus type 2 (AAV) vector containing ecotropic retroviral receptor (ecoR) cDNA under the control of the Rous sarcoma virus (RSV) long terminal repeat ...
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SARS CORONAVIRUS: AN OVERVIEW
... of recombinant adeno- associated virus encoding receptor-binding domain of severe acute respiratory syndrome coronavirus (SARS-CoV) spike protein induces strong mucosal immune responses and ...
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Adeno-Associated Virus 8-Mediated Gene Therapy for Choroideremia: Preclinical Studies in in Vitro ind in Vivo Models
... encoding recombinant adeno-associated virus serotype 8 (rAAV8) vector, which targets retinal cells efficiently, for therapeutic effect and safety in vitro and in vivo in a murine model of ...
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Generation of Neutralizing Activity against Human Immunodeficiency Virus Type 1 in Serum by Antibody Gene Transfer
... immunodeficiency virus (HIV) vaccine approaches have elicited meaningful anti- gen-specific T-cell responses in animal models, no single vaccine candidate has engendered antibodies that broadly neutralize primary ...
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A cis-Acting Element That Directs Circular Adeno-Associated Virus Replication and Packaging
... AAV lytic replication appears to proceed along two different pathways marked by the assembly of linear or circular duplex intermediates. While the origin of the conventional pathway has been well characterized, ...
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Cloning of an Avian Adeno-Associated Virus (AAAV) and Generation of Recombinant AAAV Particles
... sequencing. Virus from infected clarified allantoamniotic fluids was precipitated by centrifugation at 100,000 ⫻ g for 2 ...the virus-containing pellet was resuspended in proteinase K digestion buffer (50 ...
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Infectious Entry Pathway of Adeno-Associated Virus and Adeno-Associated Virus Vectors
... of adeno- virus, implying that AAV alone is capable of penetration into the cytosol, it was of interest to determine synergism or com- petition of endosomal escape following adenovirus and AAV ...by ...
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Adeno-associated virus type 2 DNA replication in vivo: mutation analyses of the D sequence in viral inverted terminal repeats.
... Adeno-associated virus type 2 (AAV) is a nonpathogenic human parvovirus which contains a single-stranded DNA ge- nome of 4,680 nucleotides ...helper virus such as adenovirus or herpesvirus ...
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Recombinant junctions formed by site-specific integration of adeno-associated virus into an episome.
... Although head-to-tail and tail-to-tail arrangements in chro- mosomal inserts containing multiple copies of the AAV ge- nome have been reported previously (5, 22, 26, 30, 45), the finding of head-to-tail organization of ...
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Structure of simian virus 40-adeno-associated virus recombinant genomes.
... 56, 1985 STRUCTURE OF SV 40-ADENO-ASSOCIATED VIRUS GENOMES DISCUSSION The SV40-AAV recombinants classified as group A type, which arose from cells either cotransfected with SV40 and AAV [r] ...
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