Recombinant adenovirus
Immune Responses in Macaques to a Prototype Recombinant Adenovirus Live Oral Human Papillomavirus 16 Vaccine
... oral adenovirus vaccines have properties that are well-suited for use in such ...live recombinant adenovirus vaccine prototype that produces abundant HPV16 L1 protein from the adenovirus major ...
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Persistence of recombinant adenovirus in vivo is not dependent on vector DNA replication.
... of recombinant adenovirus vectors in cultured ...E1-deleted adenovirus vector DNA accumulates in trans- formed cells as well as primary hepatocytes in culture, in a multiplicity of infection ...
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Genomic Analyses of Recombinant Adenovirus Type 11a in China
... human adenovirus type 11 (HAdV-11) strain QS, isolated in China, was conducted, and its sequence was compared with the sequences of strains within the species of ...a recombinant adenovirus of ...
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Durable Mucosal Simian Immunodeficiency Virus-Specific Effector Memory T Lymphocyte Responses Elicited by Recombinant Adenovirus Vectors in Rhesus Monkeys
... lication-incompetent recombinant adenovirus (rAd) vectors in- duces antigen-specific mucosal cellular immune responses in mice and rhesus monkeys ...
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Construction and Immunogenicity of Recombinant Adenovirus Vaccines Expressing the HMW1, HMW2, or Hia Adhesion Protein of Nontypeable Haemophilus influenzae
... (26). Recombinant adenoviral vectors are particularly attractive as vaccine candi- dates for immunization of the upper respiratory tract ...(48). Recombinant adenoviral vec- tors have been shown to be ...
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A Recombinant Adenovirus Expressing Ovine Interferon Tau Prevents Influenza Virus-Induced Lethality in Mice
... cinia virus (24), which we had previously demonstrated to inhibit ovine IFN- (data not shown). This resulted in a dose-dependent abrogation of the capacity of the IFN--containing media to in- duce an antiviral state ...
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Antitumor efficacy of a recombinant adenovirus encoding endostatin combined with an E1B55KD deficient adenovirus in gastric cancer cells
... of recombinant Ad may severely hin- der the therapeutic ...of recombinant Ad also inhibited the tumor growth by activation of direct and indirect immune response to exert “bystander effects” in ...
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Inhibition of porcine reproductive and respiratory syndrome virus infection by recombinant adenovirus and/or exosome delivered the artificial microRNAs targeting sialoadhesin and CD163 receptors
... ViraBind? Adenovirus Miniprep Kit (CELL BIOLABS, USA) by fol- lowing the manufacturer? s protocol, and titrated on AAV- 293 cells as fluorescent formation units ...
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Recombinant Mycobacterium bovis BCG Prime-Recombinant Adenovirus Boost Vaccination in Rhesus Monkeys Elicits Robust Polyfunctional Simian Immunodeficiency Virus-Specific T-Cell Responses
... Generation of rBCG. M. bovis BCG (Pasteur) was grown in 7H9 medium supplemented with 10% oleic acid-albumin-dextrose-catalase (Difco) and 0.05% Tween 80 (Fisher Scientific). Full-length SIVmac239 gag and pol and a ...
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Adenovirus mediated expression of a voltage gated potassium channel in vitro (rat cardiac myocytes) and in vivo (rat liver) A novel strategy for modifying excitability
... Excitability is governed primarily by the complement of ion channels in the cell membrane that shape the contour of the action potential. To modify excitability by gene transfer, we created a recombinant ...
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Hypercholesterolemia in low density lipoprotein receptor knockout mice and its reversal by adenovirus mediated gene delivery
... Abbreviations used in this paper: AdCMV-Luc, recombinant adenovirus containing luciferase cDNA; AdCMV-LDLR, recombinant adenovirus containing human LDL receptor cDNA; CMV, cytomegaloviru[r] ...
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Characterization of an Adenovirus Vector Containing a Heterologous Peptide Epitope in the HI Loop of the Fiber Knob
... a recombinant adenovirus ...full-size adenovirus genome cloned in the bacterial vector and flanked with two PacI ...of adenovirus genomic DNA which dictate the localization of this construct ...
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Transient gene transfer and expression of Smad7 prevents bleomycin induced lung fibrosis in mice
... nondefective adenovirus type 5 bearing a ...of recombinant adenovirus- es using adenovirus DNA-terminal protein complex and a cosmid bear- ing the full-length virus ...
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Modulation of collagen induced arthritis by adenovirus mediated intra articular expression of modified collagen type II
... produce recombinant adenoviral DNA containing rCB11-A9 or luciferase, we excised the expression cassettes from recombinant pShuttle2 plasmid DNA by digesting with I- Ceu I and PI-Sce I and ligated the ...
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Targeting Gene-Viro-Therapy with AFP driving Apoptin gene shows potent antitumor effect in hepatocarcinoma
... As shown in our study, AD55-Apoptin demonstrated a much stronger capability to induce apoptosis of hepa- tocarcinoma cells than that of ONYX-015 and AD55, however, it had no or little impair on normal cells (Fig- ures 2, ...
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Adenovirus-Platelet Interaction in Blood Causes Virus Sequestration to the Reticuloendothelial System of the Liver
... Intravenous (i.v.) delivery of recombinant adenovirus serotype 5 (Ad5) vectors for gene therapy is hindered by safety and efficacy problems. We have discovered a new pathway involved in unspecific Ad5 ...
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High-Efficiency System for the Construction of Adenovirus Vectors and Its Application to the Generation of Representative Adenovirus-Based cDNA Expression Libraries
... for adenovirus generation are outlined in ...resulting recombinant adenoviruses can be packaged efficiently into viral capsids because they contain a complete adenoviral packaging signal and, as a ...
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A Chimeric Type 2 Adenovirus Vector with a Type 17 Fiber Enhances Gene Transfer to Human Airway Epithelia
... fibrosis, recombinant adenovirus type 2 (Ad2) and Ad5 are being investigated as vectors to transfer cystic fibrosis transmembrane conductance regulator cDNA to airway ...other adenovirus serotypes at ...
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Amelioration of collagen induced arthritis by CD95 (Apo 1/Fas) ligand gene transfer
... a recombinant adenovirus-carrying FasL ...5 adenovirus with E1a, E1b, and a portion of E3 region ...The recombinant virus was then produced by cotransfec- tion of 293 cells with pAd-CMV-FasL- ...
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Induction of central tolerance by intrathymic inoculation of adenoviral antigens into the host thymus permits long term gene therapy in Gunn rats
... that recombinant adenoviral vectors are capable of transducing the great majority of hepatocytes in vivo per- mitting near complete correction of the metabolic defect in Gunn rats ...either recombinant ...
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