Recombinant Antibody
Electrochemiluminescence platform for the detection of C-reactive proteins : application of recombinant antibody technology to cardiac biomarker detection
... combined recombinant antibody technology with ECL for the first time to produce a powerful assay for the determination of the cardiac biomarker C-Reactive protein at the fg level, which to our knowledge is ...
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Multimeric recombinant antibody (scFv) for ELISA detection of allergenic walnut: an alternative to animal antibodies
... 35 Walnuts are classified as an important allergenic ingredient that can cause severe 36 reactions in sensitized individuals. To prevent unintended exposure to products 37 containing walnut, food manufacturers have the ...
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Sensitive recovery of recombinant antibody clones after their in silico identification within NGS datasets
... The isolation of ligands from large combinatorial libraries is a powerful technique allowing the discovery of bioactive molecules with a wide range of applications including diagnostics and therapeutics. This technique ...
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Production of recombinant antibody fragments in microorganisms.
... The advantages o f fed-batch procedures for increased biomass production are the subject o f many reviews on recombinant E. coli fermentation (Zabriskie and Arcuri, 1986; Yee and Blanch, 1992). Improved plasmid ...
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Recombinant antibody production evolves into multiple options aimed at yielding reagents suitable for application-specific needs
... of recombinant antibodies expressed in different ...of antibody fragments produced in mammalians, such as a VHH against GFP expressed in CHO cells and scFv-Fc frag- ments against Epstein Barr virus in ...
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A recombinant antibody against Plasmodium vivax UIS4 for distinguishing replicating from dormant liver stages
... The development of new research technologies, includ- ing in vitro infection of primary hepatocytes [12] and in vivo liver stage infections of liver-chimeric mice [13] has bolstered efforts to generate improved hypnozon- ...
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Production of recombinant antibody fragments in Bacillus megaterium
... The recombinant production of antibody frag- ments requires the folding and association of at least two different domains (VH and VL) and includes the forma- tion of disulphide ...an antibody have ...
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Recombinant antibody fragments to vascular associated targets of human tumours
... anti-His antibody was very low and so an alternative method needed to be ...monoclonal antibody to human VEGF165 (R&D Systems) as shown in figure ...
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Characterization of a Type Common Human Recombinant Monoclonal Antibody to Herpes Simplex Virus with High Therapeutic Potential
... human recombinant monoclonal antibody previously isolated by antigen selection from a phage-displayed combinatorial antibody library established from a herpes simplex virus (HSV)-seropositive ...this ...
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Fast track antibody V gene rescue, recombinant expression in plants and characterization of a PfMSP4 specific antibody
... and antibody expression declines or ceases all together [3,4], meaning that the information encoded in the variable regions (V regions) must be rescued to preserve a given antibody’s unique epitope spe- cificity ...
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Intranasal Delivery of Recombinant Parvovirus-Like Particles Elicits Cytotoxic T-Cell and Neutralizing Antibody Responses
... 6. Kilian, M., and M. W. Russel. 1994. Functions of mucosal immunoglobulins, p. 127–143. In P. L. Ogra, J. Mestecky, M. E. Lamm, W. Strober, J. R. FIG. 6. Effect of prior priming with PPV:VLP on the immunogenicity of ...
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Characterization of a Macaque Recombinant Monoclonal Antibody That Binds to a CD4-Induced Epitope and Neutralizes Simian Immunodeficiency Virus
... and recombinant sCD4, an ELISA was developed. Briefly, recombinant sCD4 and a mouse monoclonal antibody ...with recombinant SIVsm gp130 (a gift from Nancy Haig- wood), blocked, and incubated ...
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Immunodominance and Functional Activities of Antibody Responses to Inactivated West Nile Virus and Recombinant Subunit Vaccines in Mice
... E-specific antibody responses after immunization of mice with soluble protein E (sE) and isolated DIII in comparison to those after immunization with inactivated whole ...DIII antibody response to the ...
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Recombinant Monoclonal Antibody Recognizes a Unique Epitope on Varicella-Zoster Virus Immediate-Early 63 Protein
... Characterization of VZV IE63 cellular localization and protein interactions is critically dependent on the availability of well-de- fined antibodies. Previously, four anti-IE63 antibodies have been described: mouse ...
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Antibody Responses to Recombinant Polyomavirus BK Large T and VP1 Proteins in Young Kidney Transplant Patients
... BK virus (BKV)-specific immunity is critical for polyomavirus-associated nephropathy, but antibody re- sponses are incompletely defined. We compared the hemagglutination inhibition assay (HIA) with immuno- ...
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Enzyme Linked Immunosorbent Assay Employing a Recombinant Antigen for Detection of Protective Antibody against Swine Erysipelas
... five recombinant proteins of the surface protective antigen (SpaA) of Erysipelothrix rhusiopathiae were examined by indirect enzyme-linked immunosorbent assay (ELISA) with the aim of developing a reliable ...
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A Recombinant Human Monoclonal Antibody to Human Metapneumovirus Fusion Protein That Neutralizes Virus In Vitro and Is Effective Therapeutically In Vivo
... The hMPV fusion (F) protein is likely the most important target of protective immunity. Sequence analysis of the hMPV F protein shows that it is related to other paramyxovirus fusion proteins and appears to have ...
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High-Resolution Structural Characterization of a New Adeno-associated Virus Serotype 5 Antibody Epitope toward Engineering Antibody-Resistant Recombinant Gene Delivery Vectors
... specific antibody response at the ICBR by enzyme-linked immunosorbent assay (ELISA) and in-house using native dot immunoblot assays as described ...capsid antibody response, the supernatants from the ...
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Evaluation of Dot Immunogold Filtration Assay (DIGFA) By Recombinant Protein EPC1 for Anti- Echinococcus granulosus IgG Antibody.
... Recombinant EPC1 (rEPC1) was produced according to method previously reported (14). Briefly Liver and lung hydatid cysts from natu- rally infected sheep were collected from slaughterhouses in the vicinity of ...
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Single-Chain Antibody Displayed on a Recombinant Measles Virus Confers Entry through the Tumor-Associated Carcinoembryonic Antigen
... MV-HXL replicates in CEA-positive cells in the absence of CD46. Given that MV-HXL bound cells expressing CEA, we assessed its ability to infect both CD46-positive and CD46- negative, CEA-positive cells. We first compared ...
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