red fluorescent protein
Generation and characterization of a recombinant Newcastle disease virus expressing the red fluorescent protein for use in co infection studies
... unknown, this phenomenon can contribute to the low fre- quency of recombination observed for these viruses. The limited number of cells that are co-infected during NDV infection may negatively affect recombination rates. ...
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A genetically encoded Ca2+ indicator based on circularly permutated sea anemone red fluorescent protein eqFP578
... K-GECO1 protein was purified by immo- bilized metal affinity chromatography with nickel- charged Profinity resin (Bio-Rad), washed with 10 mM imidazole and eluted with 100 mM imidazole in Tris-buffered ...K-GECO1 ...
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Generation and characterization of transgenic mice expressing mitochondrial targeted red fluorescent protein selectively in neurons: modeling mitochondriopathy in excitotoxicity and amyotrophic lateral sclerosis
... deregulation, protein aggregation, and permeability transition pore activation ...RNA, protein, and lipids, and initiate many forms of cell death in mammalian cells ...
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A novel far-red fluorescent xenograft model of ovarian carcinoma for preclinical evaluation of HER2-targeted immunotoxins
... novel fluorescent model of a human ovarian carcinoma xenograft overexpressing receptor HER2, a promising molecular target of solid ...far-red fluorescent protein ...the fluorescent ...
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Recombinant Hepatitis E virus like particles can function as RNA nanocarriers
... with protein coding sequence fused in-frame with HEV 5′ region (containing cap and encapsidation signal) and investigated, if the relevant protein could be expressed and elicit an immune response in ...
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“Self-cleaving” 2A peptide from porcine teschovirus-1 mediates cleavage of dual fluorescent proteins in transgenic Eimeria tenella
... This limitation has been overcome by a small “self- cleaving” peptide, F2A, first identified by Ryan et al. in the foot-and-mouth disease virus, a member of the picornavirus group [10]. Subsequently, “2A like” peptides ...
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Specialized mouse embryonic stem cells for studying vascular development
... Notes: Tumor assays of (A–C) A3-ESC exhibiting endoderm, ectoderm, and mesoderm, respectively. (D–F) B2-ESC exhibiting endoderm, ectoderm, and mesoderm, respectively. The selected A3-ESC images show (A) elongated ...
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High frequency, cell type-specific visualization of fluorescent-tagged genomic sites in interphase and mitotic cells of living Arabidopsis plants
... sp. red fluorescent protein; EGFP or just G: enhanced green fluorescent protein; GFP: green fluorescent protein; R: DsRed2; mRed or mR: DsRed-monomer fluorescent ...
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A pink mouse reports the switch from red to green fluorescence upon Cre mediated recombination
... the red fluorescent protein mCherry to eGFP after Cre-mediated ...Both fluorescent proteins are expressed from the ubiquitously active and strong CAGGS ...the red fluorescent ...
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The Adenovirus E1b55K/E4orf6 Complex Induces Degradation of the Bloom Helicase during Infection
... BLM protein is located throughout the nucleoplasm and E1b55K is in cytoplasmic aggregates ...monomeric red fluorescent protein (mRFP) (at a 9:1 ratio), transfected cells demonstrated nuclear ...
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Targeting tumor vasculature through oncolytic virotherapy: recent advances
... RFP, red fluorescent protein; Angio, angiostatin; Ad, adenovirus; BMD, bone marrow-derived; ECs, endothelial cells; Endo, endostatin; HCC, hepatocellular carcinoma; hGM-CSF, human ...
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Influence of mesenchymal stem cells on metastasis development in mice in vivo
... Methods: MSCs were isolated from bone marrow of normal donors, characterized, and genetically labeled with luciferase (luc2). The effects of MSCs on MDA-MB-231 cancer cell proliferation were evaluated in conditioned ...
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Localization of Herpes Simplex Virus Type 1 UL37 in the Golgi Complex Requires UL36 but Not Capsid Structures
... green fluorescent protein (GFP) to follow the fate of UL37 within cells during the normal course of virus ...a fluorescent-protein-tagged UL37 polypep- ...(red fluorescent ...
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Interaction of Poxvirus Intracellular Mature Virion Proteins with the TPR Domain of Kinesin Light Chain in Live Infected Cells Revealed by Two-Photon-Induced Fluorescence Resonance Energy Transfer Fluorescence Lifetime Imaging Microscopy
... A36 protein with the cellular microtubule motor protein ...rescent protein (EGFP), fused to the C terminus of VACV A36, by monomeric red fluorescent protein (mDsRed), fused to ...
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Rab18 Facilitates Dengue Virus Infection by Targeting Fatty Acid Synthase to Sites of Viral Replication
... Rab18 protein, a member of the Rab GTPase family, key regulators of membrane trafficking, is located on the organ- elles involved in DENV infection, such as the endoplasmic reticulum (ER) and lipid droplets ...
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Epigenetics of cell free plasma DNA for non invasive prenatal diagnosis of fetal aneuploidies
... MeDIP is another method by which the methylation of DNA can be assessed. Using the antibody Anti-5-Methylcytosine can provide up to a 90 fold enrichment of methylated DNA in a dose dependent sequence dependent manner ...
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Use of green fluorescent protein color variants expressed on stable broad-host-range vectors to visualize rhizobia interacting with plants
... green fluorescent protein (GFP) or color variants thereof (henceforth collectively called auto- fluorescent proteins [AFPs]) from the lac ...cyan fluorescent protein (ECFP) or enhanced ...
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Actin Cytoskeleton Is Involved in Targeting of a Viral Hsp70 Homolog to the Cell Periphery
... FIG. 4. Effects of various drug treatments on the integrity of cytoskeleton and subcellular localization of Hsp70h-GFP or TMV MP-GFP. (A) Intact microtubules labeled by transgenically expressed GFP-tubulin in epidermal ...
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A Validated Set of Fluorescent Protein Based Markers for Major Organelles in Yeast (Saccharomyces cerevisiae)
... In addition, one incidental outcome of our study was the realization that late Golgi and early endosomes, two terms with seemly distinct identities, denote the same compartment at the resolution of light microscopy. The ...
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Anterograde Transport of Herpes Simplex Virus Capsids in Neurons by both Separate and Married Mechanisms
... large protein sequences fused to viral proteins, whereas the previous studies were done using wild-type HSV or one-color ...bulky fluorescent proteins to VP26 reduces capsid transport, and this might also ...
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