Rep protein
Roles of Adeno-Associated Virus Rep Protein and Human Chromosome 19 in Site-Specific Recombination
... one Rep-DNA complex was formed on ch-19 target ...RBE. Rep-DNA complexes involved a multimeric protein structure that spanned ...Rep68 protein are expressed per cell. Comparison of the ...
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A Chimeric Protein Containing the N Terminus of the Adeno-Associated Virus Rep Protein Recognizes Its Target Site in an In Vivo Assay
... AAV Rep pro- teins so far have utilized gel mobility shift assays to study interactions in ...whole Rep protein, the results are difficult to interpret in light of the interdependence of DNA ...
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Evidence for covalent attachment of the adeno-associated virus (AAV) rep protein to the ends of the AAV genome.
... To determine whether the AAVencoded Rep protein was the only viral protein required by cellular extracts for resolution, purified Rep68 protein was added to in vitro resolution reaction [r] ...
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The cellular transcription factor SP1 and an unknown cellular protein are required to mediate Rep protein activation of the adeno-associated virus p19 promoter.
... E1a protein and the AAV nonstructural (Rep) proteins. The Rep proteins have been shown to repress the AAV p5 promoter yet facilitate activation of the p19 and p40 promoters during a productive ...AAV ...
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Analysis of mutations in adeno-associated virus Rep protein in vivo and in vitro.
... With the exception of P365T, mutants carrying mutations within this region of the Rep protein amino acids 326 to 419 are defective for both transactivation and replication in vivo and wi[r] ...
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Identification of Cellular Proteins That Interact with the Adeno-Associated Virus Rep Protein
... with Rep, therefore, may be a redundant mechanism for in- hibiting MRN function that functions in addition to the Ad E1b and E4 proteins and may be more relevant when other helper viruses, such as herpes, are ...
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Mutational analysis of adeno-associated virus Rep protein-mediated inhibition of heterologous and homologous promoters.
... point-mutated Rep proteins on heterologous and homol- ogous ...(B) Rep protein ...Total protein was isolated, and the Rep proteins were detected by immunoblotting with a monoclonal ...
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Identification of an Adeno-Associated Virus Rep Protein Binding Site in the Adenovirus E2a Promoter
... (48). Rep protein affinity for the other transcription promoters cited has not been ...the Rep binding motif, 14 are shared between the two promoters. Rep protein binding to the AAV p5 ...
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Plant Geminivirus Rep Protein Induces Rereplication in Fission Yeast
... geminiviral Rep protein in modifying the cell cycle of plant cells upon infection (reviewed in references 31 and ...of Rep interference with the cell ...the Rep protein, as shown in ...
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A Dimeric Rep Protein Initiates Replication of a Linear Archaeal Virus Genome: Implications for the Rep Mechanism and Viral Replication
... rudivirus Rep protein subunits initiates replication by nick- ing at one of the target ori sites in the telomeric region (step 1), releasing a new 3 ⬘ DNA end which can be used as a primer to initiate DNA ...
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Studies of the Mechanism of Transactivation of the Adeno-Associated Virus p19 Promoter by Rep Protein
... a Rep-expressing plasmid re- pressed p5 transcription both in the absence (20-fold decrease) and in the presence (12-fold decrease) of Ad ...of Rep to the p5 ...by Rep expression both in the presence ...
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Identification of linear DNA sequences that specifically bind the adeno-associated virus Rep protein.
... We find that Rep68 is capable of binding to a linear DNA sequence that is contained within a 25-bp sequence of the A stem of the adeno-associated virus AAV terminal repeat proximal to th[r] ...
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Both excision and replication of cloned autonomous parvovirus DNA require the NS1 (rep) protein.
... The construction of pLudl, 4249 a LuIll virus clone with the right-end palindrome Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest When a bacterial plasmid containing th[r] ...
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Features of the adeno-associated virus origin involved in substrate recognition by the viral Rep protein.
... In addition, the enzyme may recognize the polarity of the two strands at the cut site, or it may recognize another sequence element within the stem of the hairpin that is required for co[r] ...
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Adeno-associated virus rep protein synthesis during productive infection.
... In a previous study 34 in human 293 cells coinfected with AAV and adenovirus, the two major Rep proteins Rep78 and Rep52 were detected by immunoblotting analysis earlier after infection [r] ...
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In vitro resolution of adeno-associated virus DNA hairpin termini by wild-type Rep protein is inhibited by a dominant-negative mutant of rep.
... We expressed both wild-type Rep78 and mutant Rep78NTP proteins in two helper-free expression systems consisting of either recombinant baculoviruses in insect cells or the human immunodef[r] ...
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Sequences required for coordinate induction of adeno-associated virus p19 and p40 promoters by Rep protein.
... To determine whether the p40 promoter also required noncontiguous upstream sequences, two deletions were constructed which removed the p5 promoter and part or all of Downloaded from http[r] ...
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Interaction of the adeno-associated virus Rep protein with a sequence within the A palindrome of the viral terminal repeat.
... Since the pattern of Rep-DNA complexes was not significantly different when the A stem or TR was used as a substrate, it is likely that the 10:1 ratio found for the A stem reflects the s[r] ...
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The Rep78 gene product of adeno-associated virus (AAV) self-associates to form a hexameric complex in the presence of AAV ori sequences.
... target protein and the target- binding protein results in induction of T antigen expression and subsequent T antigen-mediated replication of vector 2, which bears an SV40 origin of ...of ...
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Capsid protein expression and adeno-associated virus like particles assembly in Saccharomyces cerevisiae
... if Rep could affect the VP expression ...and Rep negative) produced VP3 protein after growth in glucose, while VP1 expression was induced only after the cell growth in galactose containing medium ...
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