reporter assay
A transcriptomic reporter assay employing neutrophils to measure immunogenic activity of septic patients’ plasma
... cell reporter system coupled with whole transcriptome readout allowed identification of a severity signature for sepsis that was highly accurate in two inde- pendent ...transcriptomic reporter assay ...
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Development of Virus-Like-Particle Vaccine and Reporter Assay for Zika Virus
... microneutralization assay using reporter virus particles (RVPs) to measure the neutralizing immune response generated in ...neutralization assay is safe, as it does not require use of live virus, and ...
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<p>Comprehensive Transcriptomic Analysis and Experimental Validation Identify lncRNA HOXA-AS2/miR-184/COL6A2 as the Critical ceRNA Regulation Involved in Low-Grade Glioma Recurrence</p>
... luciferase reporter assay, but not ...luciferase reporter assay, but not ...luciferase reporter assay, but not COL6A2-3 ′ ...luciferase reporter assay, but not ...
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MicroRNA let 7 regulates the expression of ecdysteroid receptor (ECR) in Hyalomma asiaticum (Acari: Ixodidae) ticks
... pmirGLO reporter vector interacted with let-7, the sequence that did not interact with let-7 was cloned into the pmirGLO reporter vector to create the recombinant plasmid designated ...luciferase ...
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LINC00667/miR-449b-5p/YY1 axis promotes cell proliferation and migration in colorectal cancer
... luciferase reporter assay, only YY1 down-regulation restrained the activ- ity of LINC00667 promoter in both CRC cells ...ChIP assay confirmed the affinity of YY1 to LINC00667 promoter ...
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Original Article miR-143 inhibits osteosarcoma cell proliferation by down-regulating K-ras expression
... Dual-luciferase reporter assay was performed to determine the regulatory relationship between miR-143 and Kras using wild type or mutated Kras 3’UTR inserted reporter vectors; Kras expression was ...
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Genetic analysis of GABRB3 as a candidate gene of autism spectrum disorders
... Dual-Luciferase Reporter Assay System (Pro- mega, Madison, WI, ...the reporter gene experi- ment was repeated three ...normalized reporter gene activity of each construct. Comparison of ...
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Long noncoding RNA HOTAIR promotes breast cancer development by targeting ZEB1 via sponging miR-601
... Dual-luciferase reporter assay results showed that miR-601 overexpression markedly suppressed the luciferase activity of ZEB1 3 ′ UTR-WT reporter, while had no effect on the lucif- erase activity of ...
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Involvement of Conserved Amino Acids in the C-Terminal Region of LINE-1 ORF2p in Retrotransposition
... retrotransposition reporter assay in conjunction with truncated ORF2, and ORF2 with mutations in conserved areas of the extreme C terminus of the ...
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RGS domain-independent Sst2 action
... Previous work has demonstrated that Sst2 is proteolytically cleaved in vivo at Ser-414 and Ser-416 to yield two products: the C-terminal RGS domain, and the N-terminal half of the protein containing 2 DEP domains 19 . ...
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Down-regulation of miR-27a might inhibit proliferation and drug resistance of gastric cancer cells
... luciferase reporter assay was ...D1 reporter gene led to significantly decrease in cyclin D1 promoter activity, suggesting that miR-27a might target cyclin ...
5
A novel loss-of-function mutation of GATA3 (p.R299Q) in a Japanese family with Hypoparathyroidism, Deafness, and Renal Dysplasia (HDR) syndrome
... luciferase reporter assay system confirmed that the mutation abolished the enhancing effects of wild-type GATA3 on the promoter activity of the consensus GATA responsive element and that of human PTH ...
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MaveDB: an open source platform to distribute and interpret data from multiplexed assays of variant effect
... parallel reporter assay dataset and manually inspected the publication ’ s supplementary data and methods to determine whether counts or scores for the assayed variants were ...
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PARI functions as a new transcriptional target of FOXM1 involved in gastric cancer development
... Luciferase reporter assay and chromatin immunoprecipitation assay further demonstrated that FOXM1 directly regulated PARI transcription by binding to the specific sequences of PARI ...
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RBFOX3 Promotes Tumor Growth and Progression via hTERT Signaling and Predicts a Poor Prognosis in Hepatocellular Carcinoma
... Chromatin immunoprecipitation (ChIP) assay ChIP assay was performed according to Carey’s protocol. Briefly, the cells were fixed with 1% formaldehyde, and the cross-linking was quenched by adding in 100 μl ...
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Rapid Tuberculosis Diagnosis Using Reporter Enzyme Fluorescence
... diagnostic assay was carried out in a blinded manner, wherein the personnel responsible for doing the experiments were not aware of the acid-fast stain (smear) or culture status of the patients providing ...REFtb ...
15
PA Residues in the 2009 H1N1 Pandemic Influenza Virus Enhance Avian Influenza Virus Polymerase Activity in Mammalian Cells
... The 2009 pandemic influenza virus (pH1N1) is a swine-origin reassortant containing human, avian, and swine influenza genes. We have previously shown that the polymerase complex of the pH1N1 strain A/Cali- fornia/04/2009 ...
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Measuring microRNA reporter activity in skeletal muscle using hydrodynamic limb vein injection of plasmid DNA combined with in vivo imaging
... of reporter genes in cells cultured in vitro, and none have demonstrated the ability to quantify skeletal muscle reporter gene ex- pression in ...of reporter pDNA into skeletal muscle in combination ...
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Detection of Anti Hepatitis C Virus Effects of Interferon and Ribavirin by a Sensitive Replicon System
... accurate assay system was developed using the reporter replicon system with an HCV genotype 2a subgenomic replicon (clone JFH-1) that exhibits robust replication in various cell ...This reporter ...
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A heptanucleotide sequence mediates ribosomal frameshifting in mammalian cells.
... The difference in frameshifting efficiency between our reporter gene assay system and the native HIV-1 sequence in vivo and in vitro may be explained by technical differences or by biolo[r] ...
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