reporter gene assays
![Cytokine regulation of human leukocyte antigen DR[alpha] (HLA DR[alpha]) gene expression in human tumour cell lines](data:image/gif;base64,R0lGODlhAQABAIAAAP///wAAACH5BAEAAAAALAAAAAABAAEAAAICRAEAOw==)
Cytokine regulation of human leukocyte antigen DR[alpha] (HLA DR[alpha]) gene expression in human tumour cell lines
... Reporter gene assays with HLA-DRa 680 promoter fragment CAT reporter gene assays employing the glioblastoma cell line U138MG transiently transfected with the vector pDRa680CAT demonstrat[r] ...
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Original Article DCC is expressed in a CD166-positive subpopulation of chondrocytes in human osteoarthritic cartilage and modulates CRE activity
... luciferase reporter gene assays were performed in DCC-transfected chondrocytes versus controls which showed a significant reduction of CRE activity after trans- fection of DCC (Figure 2D), whereas ...
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Epstein-Barr Virus EBNA-3C Is Targeted to and Regulates Expression from the Bidirectional LMP-1/2B Promoter
... latency gene promoters, ...LMP-2B gene on the opposite DNA strand; the common promoter for the multicistronic EBNA gene, Cp; and the LMP-2A promoter (20, 30, 39, 40, 45, ...In reporter ...
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The Epstein-Barr Virus BZLF1 Protein Interacts Physically and Functionally with the Histone Acetylase CREB-Binding Protein
... early gene promoters, BMRF1 and BHRF1, in reporter gene assays and that CBP enhances Z-mediated activation of BMRF1 from the endogenous viral ...early gene promoters con- taining ...
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journal.pone.0029542.PDF
... Viruses employ various strategies to manipulate activities of host transcription factors in their favour. To investigate the effect of SARS-CoV 3b protein on the RUNX1b transcriptional activity, reporter ...
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A single nucleotide polymorphism in the 3’-UTR of STAT3 regulates its expression and reduces risk of pancreatic cancer in a Chinese population
... regulate gene expression, we aimed at finding functional variants in this ...luciferase reporter gene assays were performed for function ...
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Measles Virus C Protein Interferes with Beta Interferon Transcription in the Nucleus
... Reporter gene assays. Cells transfected as indicated, and in some ex- periments stimulated by infection with Sendai virus (SeV) DI-H4 (kindly provided by D. Garcin) at 6 h posttransfection (p.t.), ...
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Hepatocyte nuclear factor 1 and the regulation of the human factor IX gene : a thesis presented in partial fulfilment of the requirements for the degree of Master of Science in Molecular Biology at Massey University
... binds within the -15 to +3 region of the wildtype factor IX promoter in vitro 44 • Additionally, CAT reporter gene assays indicate that HNF4 has the ability to activate transcription fro[r] ...
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Expression of Manduca sexta V ATPase genes mvB, mvG and mvd is regulated by ecdysteroids
... performed reporter gene assays in Sf21 insect ...corresponding reporter gene plasmid, we added 20-HE to the culture medium and incubated the cells for different ...
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Enhanced reporter gene assay for the detection of osteogenic differentiation
... point assays, there is increasing demand for methods allowing non-invasive kinetic differentiation ...monitoring. Reporter gene assays employing tissue-specific promoters and suitable ...
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The regulation of bovine ATP citrate lyase promoter : a thesis submitted in partial fulfilment of the requirements for the degree of Master of Science in Biochemistry, Massey University, 2006
... To investigate the molecular regulation of the bovine ATP citrate lyase gene, several experimental methods were used in this study such as reporter gene assays and electrophoretic mobili[r] ...
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Hypoxia inducible factor-1 mediates the expression of the immune checkpoint HLA-G in glioma cells through hypoxia response element located in exon 2
... Shift Assays and luciferase reporter gene assays revealed that HLA-G upregulation depends on Hypoxia Inducible Factor-1 (HIF-1) and a hypoxia responsive element (HRE) located in exon ...HLA-G ...
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Noninvasive Molecular Neuroimaging Using Reporter Genes: Part I, Principles Revisited
... well suited for use with small animal models, is relatively easily accessible to researchers in their laboratory setting, and offers particular flexibility in experimental investigations of molec- ular mechanisms of ...
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Positron Emission Tomography Reporter Genes and Reporter Probes: Gene and Cell Therapy Applications
... imaging reporter genes (IRGs) and PET reporter probes (PRPs) are amongst the most valuable tools for gene and cell ...In gene therapy, PET IRGs/PRPs can be used for whole-body imaging of ...
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In silico identification and functional validation of allele- dependent AR enhancers
... complete linkage disequilibrium with SNPs in a nearby DNase I Hypersensitive Sites [26]. Few of them were demonstrated to modulate transcription factor binding, leading to allele specific transcriptional activity. The ...
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Multimodality reporter gene imaging: Construction strategies and application
... of reporter gene transduction, the possibility of uninhibited infection and stochastic genomic insertion of exogenous reporter genes limits their clinical application ...non-viral gene ...
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Magnetic Resonance Reporter Gene Imaging
... Nevertheless, enzyme-based MR reporter gene imaging faces many challenges. One problem associ- ated with the ß-gal-based approach is that even when the enzyme has not cleaved off galactose, outer sur- face ...
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Heat Induced Reporter and Therapeutic Gene Expression in Cancer Gene Therapy
... cytokine gene therapy applications (100, ...for gene delivery after intratumoral gene injection ...enhanced gene transfer (UEGT) has also ...
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Up-regulation of human cervical cancer proto-oncogene contributes to hepatitis B virus-induced malignant transformation of hepatocyte by down-regulating E-cadherin
... HCCR gene (amino acids (aa) 1–360) into ...HCCR gene in the shuttle vector pAdTrack-CMV, recombined with Ad backbone plasmid pAdEasy-1 vector and packaged in QBI293A, a human embryonic kidney cell ...
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Regulation of the Epstein-Barr Virus C Promoter by AUF1 and the Cyclic AMP/Protein Kinase A Signaling Pathway
... 16xCBF2LUC reporter plasmid, the kinetics of promoter activation were determined after treatment with dibutyryl ...CpLUC reporter also indicated that enhancement by PKA induction required at least 24 h ...
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