Reporter gene protein expression
Heat Induced Reporter and Therapeutic Gene Expression in Cancer Gene Therapy
... Abstract Gene therapy for cancer is an important research focus, with most approved gene therapy clinical trials involving cancer ...before gene therapy will become a first line modality in the ...
136
Exploring the eukaryotic gene expression machinery using a single-cell yeast gene expression reporter
... 2: Gene expression reporter design and validation ...Eukaryotic gene expression is orchestrated by a complex, dynamic, and regulated network of protein-protein and ...
264
Towards the Development of a MagA Reporter Gene Expression Construct for Magnetic Resonance Imaging
... luciferase reporter gene expression construct using the hNISmin and investigate its induction by ...MagA gene construct using HA- and myc- epitopes cloned onto the 5ʹ′ and 3ʹ′ terminals of ...
88
Indexing TNF-αgene expression using a gene-targeted reporter cell line
... Cell-based gene reporter assay systems were developed as an alternative system amenable to HTS over 10 years ago, and have been widely used to study transcription and gene ...detectable ...
10
Evaluation of GFP reporter utility for analysis of transcriptional slippage during gene expression
... GFP protein, by constructing − 1 frameshifted gfp-1 fusion without any long slippery sequence (pETmingfp-1) and found it to manifest low fluorescence level, little above background ...GFP protein ...
10
Using a Single Fluorescent Reporter Gene to Infer Half-Life of Extrinsic Noise and Other Parameters of Gene Expression
... of gene expression and obtain information about sources of nongenetic ...of gene expression, as well as the strength and half-life of extrinsic noise from single ...
11
Regulation of Closterovirus Gene Expression Examined by Insertion of a Self-Processing Reporter and by Northern Hybridization
... A reporter open reading frame (ORF) coding for a fusion of bacterial  -glucuronidase (GUS) with a proteinase domain (Pro) derived from tobacco etch potyvirus was utilized for tagging individual genes of beet ...
6
Effect of secretory pathway gene overexpression on secretion of a fluorescent reporter protein in Aspergillus nidulans
... recombinant protein secre- tion by targeted overexpression of secretion pathway ...Some gene targets investigated here, including genes from different compartments of the secretory pathway resulted in no ...
14
A Conditional System to Specifically Link Disruption of Protein-Coding Function with Reporter Expression in Mice
... Conditional gene deletion in mice has contributed immensely to our understanding of many biological and biomedical ...within protein-coding transcripts, current gene-tar- geting approaches typically ...
9
Magnetic Resonance Reporter Gene Imaging
... ubiquitous protein which stores and releases the iron, and is activated by excess free in- tracellular iron to maintain iron ...induced expression of ferritin caused signifi- cant T2 relaxation time ...
10
Identification of Coilin Mutants in a Screen for Enhanced Expression of an Alternatively Spliced GFP Reporter Gene in Arabidopsis thaliana
... marker protein for subnuclear organelles known as Cajal bodies, which are sites of various RNA metabolic processes including the biogenesis of spliceosomal small nuclear ribonucleoprotein ...enhanced ...
36
A robust dual reporter system to visualize and quantify gene expression mediated by transcription activator-like effectors
... for gene insertion or replacement ...robust gene editing tool in a number of species ...eukaryotic gene activation or re- pression have also been reported ...single reporter system using ...
8
Activation of Herpesvirus Gene Expression by the Human Cytomegalovirus Protein pp71
... on gene expression at the RNA level in the absence of protein synthesis, an approach that would be difficult by using transfection of plasmids as reporter constructs due to the low efficiency ...
7
Down Regulation of Gene Expression by the Vaccinia Virus D10 Protein
... late gene expression (22), has led us to an ORF that decreases gene ...the protein prod- uct was required for ...the reporter gene, indicating that the mechanism did not involve ...
6
Quantitative analysis of gene expression with an improved green fluorescent protein
... fluorescent protein (GFP) for its use as a reporter to quantify promoter ...Twelve expression levels were measured using fusions of the gfp+ and lacZ genes with the tetA promoter in Escherichia ...
6
Gene Expression Protein Modification
... how gene expression of protein production can be best fits you? Soma is essential for all cells divide ...of expression can be an increase the rna processing does dna and stability of his ...
16
Exploring gene expression and protein binding data for gene regulation
... the expression level of large number of genes simultaneously, and chromatin immunoprecipitation technique followed by next generation sequencing (ChIP-Seq), which investigates the locations of proteins or ...
195
Bacteriophage P22 tail protein gene expression.
... Our results show a perfect correlation between those late gene mutations which cause the P22 tail protein to be synthesized at high levels and those which block phage assembly at or befo[r] ...
5
Probing noise in gene expression and protein production
... produce protein populations with different Fano factors F共t兲 = var关x共t兲兴 / 具x共t兲典, where var关x共t兲兴 and 具x共t兲典 are the variance and the mean of the protein concentration x共t兲, respectively ...in gene ...
9
Evidence for expression of promoterless GFP cassette: Is GFP an ideal reporter gene in biotechnology science?
... To explain the result, we hypothesized either of several reasons could be the case: a) Auto transduction of the cells with the viruses produced during the process of packaging. In this view, the viral RNA could be ...