resolution melt
Speciation of common Gram negative pathogens using a highly multiplexed high resolution melt curve assay
... High resolution melt (HRM) analysis is an end-point qPCR detection method that differentiates amplicons based on their melt profile, enables greater multiplexing capability than probe based methods ...
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Real Time PCR and High Resolution Melt Analysis for Rapid Detection of Mycobacterium leprae Drug Resistance Mutations and Strain Types
... Drug resistance surveillance and strain typing of Mycobacterium leprae are necessary to investigate ongoing transmission of leprosy in regions of endemicity. To enable wider implementation of these molecular analyses, ...
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A refined, rapid and reproducible high resolution melt (HRM) based method suitable for quantification of global LINE 1 repetitive element methylation
... The objective of the current investigation was to design a rapid, reproducible and cost-effective method to estimate overall LINE-1 methylation that is suitable for a large population-based study with limited amounts of ...
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Rapid Detection and Identification of Mucormycetes in Bronchoalveolar Lavage Samples from Immunocompromised Patients with Pulmonary Infiltrates by Use of High Resolution Melt Analysis
... Rapid differential diagnostics of pulmonary infiltrates suspected of invasive fungal disease in an immunocompromised host and early initiation of effective antifungal therapy are crucial for patient outcomes. There are ...
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Rapid identification of chloroquine and atovaquone drug resistance in Plasmodium falciparum using high resolution melt polymerase chain reaction
... In this study, two systems were used in combination (conventional thermal cycler and the LightScanner) to generate the required result. There are now systems in the market that can do PCR and HRM together such as the ...
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Rapid High Resolution Melt Analysis of Cytauxzoon felis Cytochrome b To Aid in the Prognosis of Cytauxzoonosis
... high-resolution melt analysis, this panel was predicted to distinguish cytb1 from other cytb geno- ...High-resolution melt analysis can rapidly provide prognostic information for clients ...
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Rapid Identification and Discrimination of Brucella Isolates by Use of Real Time PCR and High Resolution Melt Analysis
... 2. Briefly, targeted sequences for each marker were aligned using CLUSTAL W, and primers were designed to provide discriminating HRM profiles. The marker specificities were as follows: Bspp, all Brucella spp.; Bmel, B. ...
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High Resolution Melt PCR Analysis for Genotyping of Ureaplasma parvum Isolates Directly from Clinical Samples
... Irrespective of discrepancies in their findings, previously de- scribed assays for serovar characterization of U. parvum have been both laborious and expensive, combining the use of endpoint PCR and sequencing (11, 29) ...
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Molecular Identification of Mycobacterium avium subsp silvaticum by Duplex High Resolution Melt Analysis and Subspecies Specific Real Time PCR
... Accurate identification of mycobacterial species and subspecies is essential to evaluate their significance and to perform epide- miological studies. The subspecies of Mycobacterium avium have different attributes but ...
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Rapid Detection of Multidrug Resistant Mycobacterium tuberculosis by Use of Real Time PCR and High Resolution Melt Analysis
... High-resolution melt (HRM) analysis is a molecular tech- nique that can be used for detecting subtle genetic changes, such as SNPs conferring drug resistance in ...
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Detection of Plasmodium Species by High Resolution Melt Analysis of DNA from Blood Smears Acquired in Southwestern Uganda
... ABSTRACT Microscopic diagnosis of malaria using Giemsa-stained blood smears is the standard of care in resource-limited settings. These smears represent a potential source of DNA for PCR testing to confirm Plasmodium ...
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Direct Detection of Rifampin and Isoniazid Resistance in Sputum Samples from Tuberculosis Patients by High Resolution Melt Curve Analysis
... ABSTRACT Drug-resistant tuberculosis (TB) is a major threat to TB control world- wide. Globally, only 40% of the 340,000 notified TB patients estimated to have multidrug-resistant-TB (MDR-TB) were detected in 2015. This ...
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Genotyping of Chlamydophila psittaci by Real Time PCR and High Resolution Melt Analysis
... Human infection with Chlamydophila (Chlamydia) psittaci can lead to psittacosis, a disease that occasionally results in severe pneumonia and other medical complications. C. psittaci is currently grouped into seven avian ...
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Rapid Differentiation of Mycobacterium tuberculosis and M bovis by High Resolution Melt Curve Analysis
... Of these SNPs, the C-to-T transition at position ⫺ 215 up- stream of the GTG start codon in the promoter region of the narGHJI genes was hypothesized to be responsible for the differing nitrate reductase activities ...
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A PCR–High Resolution Melt Assay for Rapid Differentiation of Nontypeable Haemophilus influenzae and Haemophilus haemolyticus
... sequencing. There was 1°C separation between the highest melt curve for the NTHi variants (74.6°C) and the lowest melt curve for H. haemolyticus variants (75.6°C). Furthermore, as shown in Fig. 1 and 2, the ...
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A comparison of deformed wing virus in deformed and asymptomatic honey bees
... High Resolution melt plot of DWV RdRp RT-PCR products amplified from Brazilian deformed bees (red) and asymptomatic bees from cells infested with (yellow) and without Varroa ...All melt peaks are in ...
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Integrated Microfluidic Card with TaqMan Probes and High Resolution Melt Analysis To Detect Tuberculosis Drug Resistance Mutations across 10 Genes
... ABSTRACT Genotypic methods for drug susceptibility testing of Mycobacterium tuberculosis are desirable to speed the diagno- sis and proper therapy of tuberculosis (TB). However, the numbers of genes and polymorphisms ...
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Melt Crystallization pdf
... (Japan) developed in the 1970s a pressure-crystalliza- tion process, which was applied for the separation of p-cresol from its isomers on a commercial scale. A given amount of slurry from a chiller is fed to a ...
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Preparation of metal powders from silver melt for 3D printing by melt dispersion method
... Dispersion of molten metal by high-pressure water jet eliminates the formation of gas pores and obtain a powder particle shape close to spherical, due to the action of surface tension forces. Previous studies [6, 11, 12] ...
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Effect of Laser Power on Surface Finish during Laser Metal Deposition Process
... a melt pool on the substrate and then the Ti6Al4V powder was then delivered into the melt pool through the coaxial powder nozzles attached to the ...
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