Respiratory samples
Direct Sequencing of Legionella pneumophila from Respiratory Samples for Sequence Based Typing Analysis
... clinical samples, the profiles are usually derived from sputum ...from respiratory samples rarely reaches more than 20%, even when highly experienced laboratory personnel perform the isolation ...
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Quality control implementation for universal characterization of DNA and RNA viruses in clinical respiratory samples using single metagenomic next generation sequencing workflow
... clinical respiratory samples from patients with ARIs caused by a broad panel of DNA and RNA vi- ruses or of unknown etiology were analyzed in a single mNGS ...positive samples (mean = 61.3%) and ...
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Application of a Fluorogenic PCR Assay for Typing and Subtyping of Influenza Viruses in Respiratory Samples
... A fluorogenic PCR-based method (TaqMan-PCR) was developed for typing and subtyping of influenza virus genomes in clinical specimens. The TaqMan-PCR employs a probe technology that exploits the endogenous 5 ⴕ –3 ⴕ ...
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<p>Evaluating the clinical significance of nontuberculous mycobacteria isolated from respiratory samples in Iran: an often overlooked disease</p>
... Results: A total of 478 were con fi rmed to have respiratory samples which were culture positive for mycobacteria, with the prevalence of NTM infection obtained as 53 (11.1%). Overall, Mycobacterium (M.) ...
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Antimicrobial Susceptibilities of Aerobic Isolates from Respiratory Samples of Young New Zealand Horses
... of samples, with a majority of samples submitted from the Waikato region of NZ ...of samples originated from the South Island (Table 1), any true regional differences in antimi- crobial susceptibility ...
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Screening Respiratory Samples for Detection of Human Rhinoviruses (HRVs) and Enteroviruses: Comprehensive VP4 VP2 Typing Reveals High Incidence and Genetic Diversity of HRV Species C
... acute respiratory tract infections ...diagnostic respiratory samples was performed by using nested primers for the efficient amplification of the VP4-VP2 region of HRV (and enterovirus) species and ...
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Study and interest of cellular load in respiratory samples for the optimization of molecular virological diagnosis in clinical practice
... Cell quantification was achieved by amplification and detection of a human household gene in real-time PCR (hypoxanthine phosphoribosyl transferase-1) [15, 16]. Cell quantification was performed on a LightCycler 480 II® ...
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Detection of Human Respiratory Syncytial Virus in Respiratory Samples by LightCycler Reverse Transcriptase PCR
... human respiratory syncytial virus (hRSV) infections has traditionally been per- formed by virus isolation in cell culture and the direct fluorescent-antibody assay ...aspirate samples from patients with ...
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Metagenomic Nanopore Sequencing of Influenza Virus Direct from Clinical Respiratory Samples
... collected respiratory samples from the clinical microbi- ology laboratory at Oxford University Hospitals NHS Foundation Trust, a large tertiary referral teaching hospital in Southeast ...2018. ...
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Human polyomavirus type six in respiratory samples from hospitalized children with respiratory tract infections in Beijing, China
... plasma samples from healthy adult blood donors was BKPyV (82 %), JCPyV (39 %), KIPyV (55 %), WUPyV (69 %), MCPyV isolate 350 (25 %); and MCPyV isolate 339 (42 ...
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Molecular Quantification of Respiratory Syncytial Virus in Respiratory Samples: Reliable Detection during the Initial Phase of Infection
... though samples were taken by well-trained nursing staff and immediately transported to the laboratory by a dedicated investigator, the possibility of some personal variation in sampling procedures cannot be fully ...
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Amplicon Sequencing and Improved Detection of Human Rhinovirus in Respiratory Samples
... To improve our ability to diagnose rhinoviral infections, we then developed a real-time RT-PCR that could overcome the diversity that exists among picornaviruses and enteroviruses. In order to achieve this goal we used ...
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Development and Evaluation of a Quantitative, Touch Down, Real Time PCR Assay for Diagnosing Pneumocystis carinii Pneumonia
... in respiratory specimens from patients with ...98 respiratory samples (49 lower respiratory tract specimens and 49 oral washes), from 51 patients with 24 episodes of PCP and 34 episodes of ...
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High Incidence but Low Burden of Coronaviruses and Preferential Associations between Respiratory Viruses
... of respiratory viruses (6, 7), since these molecular techniques are rapid, sensitive, and can be performed on a wide variety of respiratory ...of respiratory viruses, including influenza virus A and ...
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Rapid research response to the 2009 A(H1N1)pdm09 influenza pandemic (Revised)
... isolates, respiratory samples (nasal wash, throat swab, nasopharyngeal swabs and/or nasal swabs), serum and stool samples collected from A(H1N1)pdm09 -infected subjects and subjects without ...
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Development and Preliminary Evaluation of a Rapid Oligochromatographic Assay for Specific Detection of New Human Influenza A H1N1 Virus
... The dipstick device carries two specific probe pairs for the detection of 2009 H1N1 and internal control PCR products. Each probe pair consists of one probe conjugated with colloidal gold (gold probe) and another probe ...
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Multicenter Evaluation of the Abbott LCxMycobacterium tuberculosis Ligase Chain Reaction Assay
... for respiratory and nonrespiratory specimens by using the resolved data set to show the correlation of sensitivity and specificity over a range of cutoff ...for respiratory specimens (of which this study ...
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Molecular Diagnosis of Invasive Aspergillosis and Detection of Azole Resistance by a Newly Commercialized PCR Kit
... and respiratory samples according to the manufacturer’s protocol, using the MycoGENIE DNA extraction kit for AutoMag solution (Ademtech, Pessac, ...12 samples per run in a DNA extraction plate (a ...
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A retrospective review of a tertiary Hospital’s isolation and de isolation policy for suspected pulmonary tuberculosis
... chronic respiratory and/or constitutional symptoms such as prolonged cough and loss of weight, and those with chest radiograph findings suspicious of active ...consecutive respiratory samples have ...
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Rapid diagnosis of respiratory syncytial virus infections in immunocompromised adults
... of respiratory syncytial virus (RSV) infections are widely used with pediatric patients, these tests have not been prospectively evaluated in immunocompromised (IC) ...of respiratory symptoms, ...
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