Restriction-fragment length polymorphism study of
Evaluation of PCR Restriction Profile Analysis and IS2404 Restriction Fragment Length Polymorphism and Amplified Fragment Length Polymorphism Fingerprinting for Identification and Typing of Mycobacterium ulcerans andM marinum
... marinum. The results of PRPA on a set of geographically diverse M. ulcerans isolates showed three different PRPA pro- files (Table 2): subtype 1, representing the African strains; subtype 2, representing the Australian ...
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Study of Restriction Fragment Length Polymorphism Analysis and Spoligotyping for Epidemiological Investigation ofMycobacterium bovis Infection
... 1999 Restriction fragment length polymorphism (RFLP) analysis with probes derived from the insertion element IS6110, the direct repeat sequence, and the polymorphic GC-rich sequence (PGRS) and ...
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Differentiation of Mycobacterium tuberculosis isolates by spoligotyping and IS6110 restriction fragment length polymorphism
... formed with extracts of sputum or bronchoalveolar lavage sam- ples. This provides the clinician with information on whether the patient has been infected by nosocomial transmission of a particular strain or strains known ...
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Species Identification of Mycobacteria by PCR Restriction Fragment Length Polymorphism of therpoB Gene
... PCR-restriction fragment length polymorphism analysis (PRA) using the novel region of the rpoB gene was developed for rapid and precise identification of mycobacteria to the species ...with ...
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Capillary Electrophoretic Restriction Fragment Length Polymorphism Patterns for the Mycobacterial hsp65 Gene
... two restriction enzymes (BstEII and HaeIII) and the separation of restriction fragments by 3% agarose gel ...However, restriction fragments shorter than 60 bp were not available in the diagnostic ...
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Genetic Identification of the Main Opportunistic Mucorales by PCR Restriction Fragment Length Polymorphism
... Received 9 September 2005/Returned for modification 20 October 2005/Accepted 3 January 2006 Mucormycosis is a rare and opportunistic infection caused by fungi belonging to the order Mucorales. Recent reports have ...
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Identification of Infectious Agents in Onychomycoses by PCR Terminal Restriction Fragment Length Polymorphism
... this study, we describe a PCR-terminal restriction fragment length polymorphism (TRFLP) assay to directly and routinely identify the infecting fungi in ...specific restriction ...
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Restriction fragment length polymorphism of the periplasmic flagellar flaA1 gene of Serpulina species.
... In this study we compared 41 reference and field isolates of intestinal spirochetes representing S. hyodysenteriae, S. inno- cens, S. pilosicoli, B. aalborgi, and nonclassified WBHIS by using RFLP of the PF flaA1 ...
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RESTRICTION FRAGMENT LENGTH POLYMORPHISM ANALYSIS OF RHIZOBIUM SP ISOLATED FROM DOLICHOUS LABLAB
... The efficient bean nodulating Rhizobium strains were characterized by RFLP. RFLP analysis of bacterial strain revealed the various changes in genotype of the Rhizobium sp. that taken for this study. There was ...
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Identification of 54 Mycobacterial Species by PCR Restriction Fragment Length Polymorphism Analysis of the hsp65Gene
... of restriction fragments as deduced by sequence analysis and allowed identification of mycobacteria whose PRA pat- terns were unequivocally identified by fragments shorter than 60 ...our study, the size ...
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Discrimination of Human Pathogenic Subspecies of Francisella tularensis by Using Restriction Fragment Length Polymorphism
... In this study an alternative view of the organization of the genomes from different strains of F. tularensis is offered. Entire genomes can be compared with each other on the basis of the numbers and distributions ...
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Identification of Staphylococcus spp by PCR Restriction Fragment Length Polymorphism Analysis of dnaJ Gene
... present study has demonstrated that PCR-RFLP analysis of the dnaJ gene with XapI or Bsp143I digestion proved to be an adequate tool for the correct iden- tification of almost all prevalent species and subspecies ...
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Restriction fragment length polymorphism of rRNA genes for molecular typing of members of the family Legionellaceae
... ACKNOWLEDGMENTS We gratefully acknowledge the following persons for supplying strains for the study: P. H. Edelstein, Hospital of The University of Pennsylvania, Philadelphia; B. Wentworth, Michigan State Health ...
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Amplified Fragment Length Polymorphism Analysis versus Macro Restriction Fragment Analysis for Molecular Typing ofStreptococcus pneumoniae Isolates
... this study represents the time elapsed in the normal setting of a routine diagnostic lab and was com- parable for both ...DNA restriction, and running of the ...
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DNA fingerprinting of Bacillus cereus from diverse sources by restriction fragment length polymorphism analysis
... present study, antibiotic resistance, heavy metal tolerance & molecular typing of Bacillus cereus from diverse sources such as soil, sewage water, air, fresh water, sea water and milk were ...
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Stability of IS6110 Restriction Fragment Length Polymorphism Patterns of Mycobacterium tuberculosisStrains in Actual Chains of Transmission
... present study were obtained over time spans of up to 29 months, indicating that for periods of more than 2 years IS6110 fingerprint clusters based on identical isolates can account for nearly all cases of recent ...
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Association between a T cell receptor restriction fragment length polymorphism and systemic lupus erythematosus
... J Clin Invest. 1990;86(6):1961-1967. https://doi.org/10.1172/JCI114930. The present study was designed to test the possibility that T cell receptor genes are associated/linked to those involved in systemic lupus ...
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Evolution of the IS6110 Based Restriction Fragment Length Polymorphism Pattern during the Transmission of Mycobacterium tuberculosis
... Department of Medicine, McGill University Health Centre, Montreal H3G 1A4, Canada 3 Received 25 October 2001/Returned for modification 4 December 2001/Accepted 21 January 2002 Interpretation of the molecular ...
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Detection of Rickettsia japonica in Haemaphysalis longicornis ticks by restriction fragment length polymorphism of PCR product
... japonica. Among 19 ticks collected at sites A and B, hemo- lymph preparations from 6 ticks were demonstrated to contain fluorescein-stained, rod-shaped organisms (Fig. 5). The remaining ticks of H. longicornis together ...
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Detection and Differentiation of Old World Orthopoxviruses: Restriction Fragment Length Polymorphism of thecrmB Gene Region
... RESULTS AND DISCUSSION crmB region PCR amplification of different OPV species. As shown in Fig. 1, the consensus primers VL2N and VL33 suc- cessfully amplified the crmB region from DNA of eight CPV, two ECT, one TPV, ...
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