Results of 454 sequencing a cDNA library produced from a
454 Sequencing System Software Manual Version 2.6
... the results can be controlled by command line options and configuration parameters (paralleling the equivalent controls in the GUI ...intermediate results on existing sequencing Runs to determine if ...
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454 sequencing of pooled BAC clones on chromosome 3H of barley
... a 454 parallel genome ...derived from genetically mapped ESTs on chromosome 3H was used for clone selection in a BAC library developed from cultivar “Haruna ...pooled library and mapped ...
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Identification of novel SNPSTRs by 454 sequencing in Nguni and Sotho-Tswana populations
... been produced (Kayser et al, 2002; Butler, 2003; Jobling & Tyler-Smith, ...often results in profiles where the female alleles mask the alleles of the male contributor (Shewale et ...kits results ...
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Sequencing and de novo analysis of a coral larval transcriptome using 454 GSFlx
... larvae from the coral Acropora millepora. Results: More than 600,000 reads produced in a single 454 sequencing run were assembled into ~40,000 contigs with five-fold average ...
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Automating the annotation and discovery of MicroRNA in multi-species high-throughput 454 Sequencing
... 5.3.3 Ambiguity when removing known non-miRNA elements Extracting known non-miRNA sequences is the most ambiguous of the steps. In general, it is atradeoff between removing too much and removing too little. Initially, a ...
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Comparison between Normalised and Unnormalised 454-Sequencing Libraries for Small-Scale RNA-Seq Studies.
... normalised cDNA libraries compared to unnormalised libraries in RNA-Seq ...normalised cDNA can be used in characterising expression variation due to a correlation between the relative number of reads per ...
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QTrim : a novel tool for the quality trimming of sequence reads generated using the Roche/454 sequencing platform
... Upon comparison with all other approaches, QTrim performs equally as well as the best of these methods (PRINSEQ). The trimmed reads output by PRINSEQ are, on average, slightly longer than those output by QTrim (Figure ...
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A general method to eliminate laboratory induced recombinants during massive, parallel sequencing of cDNA library
... or cell lines, RNA extraction and conversion of RNA to cDNA (RT-PCR) and cDNA amplification. Mutations in the form of insertions, deletions or substitutions could occur during any of these procedures and ...
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Application of different DNA extraction procedures, library preparation protocols and sequencing platforms: impact on sequencing results
... Regarding the three genomes obtained from DNA2, no genes or a lower number related to antimicrobial resistance were detected. This result con firmed that the initial DNA extract for this sample was degraded, as ...
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Castor bean lectins : construction and sequencing of cDNA clones
... universal sequencing primer at 1 ...batch produced overlapping sequencing ladders, and 'stuttering' was conmonly observed at the bottoms of the ...further from the insert than does the BRL ...
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Purification and cDNA sequencing of C4l: An actin-associated protein
... and Sequencing It is possible to sequence double stranded DNA directly from PCR reactions ...the sequencing ladders produced by this technique are not consistently satisfactory due to the high ...
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Cloning and sequencing of complete τ-crystallin cDNA from embryonic lens of Crocodylus palustris
... and sequencing of the complete τ-crystallin cDNA from embryonic eye lenses of a reptilian species, the Indian crocodile Crocodylus ...τ-crystallin cDNA is identical to the α-enolase ...
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Cloning of a cDNA Encoding Chitotriosidase, a Human Chitinase Produced by Macrophages
... corresponding cDNA. The nucleotide sequence of the cloned cDNA predicts a protein with amino acid sequences identical to those established for purified chi- ...cloned cDNA, confirming its identity as ...
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Construction of cDNA library and preliminary analysis of expressed sequence tags from Siberian tiger
... full-length cDNA library from Siberian tiger, Panthera tigris altaica, the most well-known wild ...extracted from cultured Siberian tiger fibroblasts in ...recombinants from unamplified ...
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Generation and analysis of expressed sequence tags from a cDNA library of the fruiting body of Ganoderma lucidum
... sequences from the G. lucidum fruiting body cDNA library is shown in Figure ...EST sequencing scale is limited, it provides some information about the expressed transcript profile of the ...
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Increasing the library size in cDNA display by optimizing purification procedures
... of cDNA display. (A) Scheme of cDNA display ...The produced mRNA-protein fusion molecule and the remaining mRNA-linker conjugate were captured with SA-beads from the translation reaction ...
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Construction and analysis of cotton (Gossypium arboreumL ) drought related cDNA library
... suppression-subtracted cDNA library Tester and driver cDNAs were reversely transcribed from the mRNA of the two sample pools, and the yield of dou- ble-stranded cDNA depended on the RNA ...of ...
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An Ambystoma mexicanumEST sequencing project: analysis of 17,352 expressed sequence tags from embryonic and regenerating blastema cDNA libraries
... Two cDNA libraries, one made from stage 18-22 embryos and the other from day-6 regenerating tail blastemas, generated 17,352 ...sequences. From the sequenced ESTs, 6,377 contigs were assembled ...
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A Universal Next-Generation Sequencing Protocol To Generate Noninfectious Barcoded cDNA Libraries from High-Containment RNA Viruses
... derived from Illumina MiSeq, the SOP can be applied to multiple platforms, including Illumina HiSeq and Ion Torrent Personal Genome Machine ...result from this study was the observation that both the ...
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Optimization and comparison of different methods for RNA isolation for cDNA library construction from the reindeer lichen Cladonia rangiferina
... or secondary metabolites. Modifying the TRIzol proce- dure by adding a second chloroform extraction step and replacing the isopropanol precipitation with ethanol pre- cipitation slightly improved the A 260/280 ratio ...
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