Reverse transcriptase, real-time polymerase chain
Monitoring PRRSV-1 in suckling piglets in an endemic herd using reverse transcriptase quantitative real time polymerase chain reaction: comparison of the rate of detection in serum and oral fluid samples and evaluation of pooling
... by reverse transcription real-time PCR Before ribonucleic acid (RNA) extraction, cOF samples were clarified by centrifugation at 1000 g for 10 ...
8
A duplex real time reverse transcriptase polymerase chain reaction assay for detecting western equine and eastern equine encephalitis viruses
... duplex real-time RT-PCR assay by adjusting the concentrations of primers and ...duplex real time RT-PCR assay we developed was 10-fold more sensitive for both WEEV and EEEV ...
5
Improved methods using the reverse transcriptase polymerase chain reaction to detect tumour cells
... efficiency limits the amount of RNA and consequently blood volume that may be analysed in any single PCR. Amplification of poly-A + RNA isolated from whole blood total RNA was more sensitive than analysis of total RNA ...
7
Detection of carcinoembryonic antigen messenger RNA in blood using quantitative real time reverse transcriptase polymerase chain reaction to predict recurrence of gastric adenocarcinoma
... The current study was retrospective analysis, and patients who should receive adjuvant chemotherapy were not set ahead of time. Generally, patients who had positive lymph node were recommended to receive adjuvant ...
8
A novel duplex real time reverse transcriptase polymerase chain reaction assay for the detection of hepatitis C viral RNA with armored RNA as internal control
... duplex real-time RT-PCR for the 109 serum samples, which were identical to those of the CAP/CTM assay, should be ...duplex real-time RT-PCR assay was ...duplex real-time RT-PCR ...
9
RT-PCR Fact Sheet Real Time Reverse Transcription Polymerase Chain Reaction (Real Time RT-PCR) Test.
... (polymerase chain reaction) tests used to determine the presence of the SARS-CoV-2 virus and help diagnose cases of COVID-19 cannot distinguish between the virus and harmless fragments of the ...
8
Expression analysis of three isoforms of hyaluronan synthase and hyaluronidase in the synovium of knees in osteoarthritis and rheumatoid arthritis by quantitative real time reverse transcriptase polymerase chain reaction
... a 7700 sequence detector in real time when the annealed probes were broken by DNA polymerases during the polymerization period. The threshold cycle numbers (C T ), from which the logarithmic amplification ...
7
Detection of Norwalk virus in stool specimens by reverse transcriptase polymerase chain reaction and nonradioactive oligoprobes
... For agarose gels, 21 coded stool specimens were purified by Sephadex G-200 spin-column chromatography, amplified by RT-PCR by using Norwalk virus NV-5'Pol and NV-3'Pol primers (left lane[r] ...
7
An analysis of the subtypes of dengue fever infections in Barbados 2003–2007 by reverse transcriptase polymerase chain reaction
... Conclusion In conclusion, it would therefore be expected that with the predominance of DENV-3 circulating in the population, although this infection may not be severe as previous when DENV-2 predominated, there will be a ...
6
Canine Distemper Virus detection based in Hemaglutinine Gene as target in Reverse Transcriptase-Polymerase Chain Reaction
... Canine Distemper have become a major concern within the veterinary clinical work. Thus, the appearance of many cases of canine distemper in adult animals with their vaccination plan up to date has alarmed veterinarians. ...
8
Diagnosis of Foot-and-Mouth Disease Virus by Real Time Reverse Transcription Polymerase Chain Reaction Assay in Iran
... various reverse transcription polymerase chain reaction (RT- PCR) methods are used to detect and identity FMDV isolations ...(13). Reverse transcription polymerase chain method ...
9
Development and Evaluation of an Enterovirus D68 Real Time Reverse Transcriptase PCR Assay
... While there are no specific treatments for EV-D68 and cur- rently no antiviral targets available, rapid and accurate diagnosis of current and future EV-D68 infections is of great concern to clinicians and public health ...
7
Deteksi Dan Identifikasi Chrysanthemum Stunt Viroid Pada Tanaman Krisan Menggunakan Teknik Reverse Transcriptase Polymerase Chain Reaction (Detection and Identification of Chrysanthemum Stunt Viroid on Chrysanthemum Using Reverse Transcriptase Polymerase
... Katakunci: Chrysanthemum stunt viroid (CSVd); Reverse transcriptase polymerase chain reaction; Dendranthema grandiflora Kitam; DNA sequencing ABSTRACT. Chrysanthemum stunt viroid (CSVd) is one ...
8
Rapid detection and typing of dengue viruses from clinical samples by using reverse transcriptase polymerase chain reaction
... As previously stated, the RNA isolated from these infected mosquitoes was originally found negative for dengue type 2 virus by the RT-PCR assay at all time points. However, when the RNA [r] ...
7
Quantitative determination, by real time reverse transcription polymerase chain reaction, of aromatase mRNA in invasive ductal carcinoma of the breast
... Real-time reverse transcription polymerase chain reaction PCR was performed using a LightCycler. The PCR reac- tion was carried out in a 20 µ l final volume containing the following: H ...
7
Development of Real Time Reverse Transcriptase PCR Assays To Detect and Serotype Dengue Viruses
... Advances in the development of fluorophores and nucleo- tide-labeling chemistries have resolved the need for postam- plification manipulations required by conventional RT-PCR and have provided the capability to conduct ...
10
Hot start reverse transcriptase: an approach for improved real-time RT-PCR performance
... one-step real-time RT-PCR assay for the detection of Middle East respiratory syndrome coronavirus (MERS- CoV) to investigate the potential of a hot start RT for improved real-time RT-PCR ...
5
Real-time quantitative PCR (polymerase chain reaction)- page 47
... B.) Fig.16B: Values of hemoglobin level (g/l) are shown as function of time during lenalidomide (LEN) treatment of female MDS patient del(5q) and trisomy 8 in different clones. The patient reached complete ...
New Developments in Quantitative Real-time Polymerase Chain Reaction Technology
... Despite this technical limitation, HDA-based amplification is increasingly being viewed as an attractive alternative in any amplification system, where length of the amplicon is less than 200 bp. One excellent example of ...
6
Removal of inhibitory substances from human fecal specimens for detection of group A rotaviruses by reverse transcriptase and polymerase chain reactions
... Silver-stained polyacrylamide gel electrophoresis of RT-PCR products after CF11 purification of model tissue culture series extracts and model fecal series extracts. Model tissue culture[r] ...
8