reverse transcription
Reverse Transcription Mechanically Initiates HIV-1 Capsid Disassembly
... that reverse transcription may occur more rapidly owing to enhanced dNTP ...the reverse transcription inhibitor, efavirenz, was added to the buffer, or when dNTPs were excluded from the ...
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Suppression of HIV-1 Infection by APOBEC3 Proteins in Primary Human CD4+ T Cells Is Associated with Inhibition of Processive Reverse Transcription as Well as Excessive Cytidine Deamination
... on reverse transcription using ERT reactions by means of a novel assay that identifies the 3= termini of nascent ...of reverse transcription contribute to the antiviral activity of endogenous ...
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Interaction between Reverse Transcriptase and Integrase Is Required for Reverse Transcription during HIV-1 Replication
... for 2 h at room temperature to remove contaminating plasmid DNA. Two million CEM cells were infected with 100 ng of the p24 equivalent of DNase-treated viruses. Infected cells were pelleted by centrifugation at 16 h ...
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Mechanisms downstream of reverse transcription reduce serum levels of HBV DNA but not of HBsAg in chronic hepatitis B virus infection
... Conclusions: The marked reduction of HBV DNA in serum after loss of HBeAg appears to be due to combined reduction of cccDNA, pgRNA and yet unidentified mechanisms downstream of reverse transcription. Such ...
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Cooperation between Reverse Transcriptase and Integrase during Reverse Transcription and Formation of the Preintegrative Complex of Ty1
... that VLPs containing full-length active IN and RT from the pJEFWTneo plasmid were able to induce transposition. As shown in Fig. 4, His ⫹ prototrophs were generated in this con- trol experiment. To control that the His ⫹ ...
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Multicenter Evaluation of a Transcription Reverse Transcription Concerted Assay for Rapid Detection of Mycobacterium tuberculosis Complex in Clinical Specimens
... the transcription-reverse transcription concerted reaction (TRC-2), which enabled one-step amplification and real-time detection of the Mycobacterium tuberculosis 16S rRNA target directly in clinical ...
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Ty3 Integrase Is Required for Initiation of Reverse Transcription
... CA, reverse transcriptase (RT), and IN (CA-RT-IN) complemented transposition of catalytic site and nuclear localization signal mutants, increased the amount of cDNA in some of the mutants, and complemented ...
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When is it time for reverse transcription to start and go?
... After cell infection, the virion core is released into the cytoplasm where it is believed to undergo structural alter- ations giving rise to a large ribonucleoprotein structure called the reverse ...
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Isolated HIV 1 core is active for reverse transcription
... Whether purified HIV-1 virion cores are capable of reverse transcription or require uncoating to be activated is currently controversial. To address this question we purified cores from a virus culture and ...
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Regulation of Hepadnavirus Reverse Transcription by Dynamic Nucleocapsid Phosphorylation
... hepadnavirus reverse tran- scription within its capsid ...retroviral reverse transcription (3, 11), that similarly must occur within an enclosed shell or ...entire reverse transcription ...
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Reverse transcription PCR detection of hepatitis G virus
... the reverse transcription-PCR (RT- PCR) assay with the Enzymun-Test DNA detection system (Boehringer Mannheim GmbH, Mannheim, Germany) and made this technique applicable for mass screening in the clin- ical ...
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Applications of Competitor RNA in Diagnostic Reverse Transcription PCR
... Detection of RNA viruses by reverse transcription (RT)-PCR has proven to be a useful approach for the diagnosis of infections caused by many viral pathogens. However, adequate controls are required for each ...
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Human Immunodeficiency Virus Type 1 Integrase Protein Promotes Reverse Transcription through Specific Interactions with the Nucleoprotein Reverse Transcription Complex
... composition, reverse transcription, nuclear import, and ...of reverse transcription in infected cells and that this function does not require the IN protein to be enzymatically (integration) ...
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Primer tRNAs for reverse transcription.
... FIG. 3. Reverse transcription of retroviral genomic RNA into double- stranded proviral DNA. This figure is modified with permission from the authors of reference 77a. Step 1: annealing of primer tRNA to the ...
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Evidence for the Packaging of Multiple Copies of Tf1 mRNA into Particles and the trans Priming of Reverse Transcription
... initiating reverse transcription that may be unique to elements that are self- priming ...prime reverse transcription in ...The reverse transcription of retroviruses has been ...
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A Mutant Tat Protein Inhibits HIV-1 Reverse Transcription by Targeting the Reverse Transcription Complex
... defective reverse transcription ...cell-based reverse transcription assays (10, 14), we propose that Nullbasic inhibits reverse transcription by disrupting assembly of the ...
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HIV 1 associated PKA acts as a cofactor for genome reverse transcription
... PKA inhibitors are less efficient at producing (−)ssDNA in ERT reaction. To examine whether such defects were linked to modification of the integrity of viruses engaged in the reaction, ERT experiments were repeated and ...
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Real time reverse transcription PCR based sequencing independent pathotyping of Eurasian avian influenza A viruses of subtype H7
... Here, reverse transcription real-time PCR assays (RT-qPCR) were developed and evaluated that allow LP and HP pathotype identification and distinction by probe-assisted detection of the ...
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Reverse transcription, loop mediated isothermal amplification assay for the sensitive and rapid detection of H10 subtype avian influenza viruses
... We see several obvious advantages of the RT-LAMP assay: 1) The specificity and sensitivity are greatly enhanced by six primers recognizing eight independent target se- quence regions, whereas conventional RT-PCR primers ...
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Blocking premature reverse transcription fails to rescue the HIV 1 nucleocapsid mutant replication defect
... cause reverse transcription to initi- ate much earlier than in wild-type ...initiated reverse transcription, and so this process is apparently occurring prematurely in the viral replication ...
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