reverse transcription quantitative real-time PCR
Validation of a primer optimisation matrix to improve the performance of reverse transcription – quantitative real time PCR assays
... of reverse transcription – quantitative real-time PCR (RT-qPCR) platforms that can simultaneously measure the expression of multiple genes is dependent on robust assays that ...
5
Use of FTA Cards To Transport Throat Swabs and Oral Fluid Samples for Molecular Detection and Genotyping of Measles and Rubella Viruses
... surveillance. Reverse cold chain requirements for the transportation of sam- ples to reference laboratories are challenging in resource-limited ...(reverse transcription-quantitative ...
11
Serum microRNA expression signatures as novel noninvasive biomarkers for prediction and prognosis of muscle-invasive bladder cancer
... MicroRNAs (miRNAs) are a subset of small non- coding RNAs (typically 19-25 nucleotides in length) that increase and/or decrease gene expression at the post- transcriptional level [6, 7]. Different miRNAs could function ...
10
Real Time Reverse Transcription PCR Assay for Comprehensive Detection of Human Rhinoviruses
... recently, real-time RT-PCR assays have been described for HRVs/HEVs (8, 9, 25, 38, 44) that offer potentially rapid, sensitive, and quantitative results and that are less prone to amplicon ...
7
Original Article Bbc3 is negatively correlated with epithelial-mesenchymal transition and is a prognostic biomarker for patients with rectal cancer
... Abstract: The role of Bbc3 in rectal cancer (RC) and correlation with Epithelial Mesenchymal Transition (EMT) re- mains unclear. The expression of Bbc3 in 30 fresh frozen RC and matched normal tissues were evaluated by ...
10
Research Progress on Reference Genes of Insect for Quantitative Real time Reverse Transcription PCR (RT qPCR)
... For reliable results, must select the appropriate internal reference genes to deter error such as inaccurate quantification of RNA, the quality of the RNA, and difference during cDNA synthesis that can trigger variations ...
9
Real Time PCR and Real Time RT PCR applications in food labelling and gene expression studies
... to real-time PCR and later, real-time reverse transcription PCR (Real-Time ...conduct PCR detection of amplified gene products and expression ...
7
Real Time Reverse Transcription PCR Assay Panel for Middle East Respiratory Syndrome Coronavirus
... One-Step quantitative RT-PCR system (Life ...III reverse transcriptase/Plat- inum Taq DNA polymerase, ...and reverse primers, ...Dx real-time PCR instrument (Life ...for ...
9
Critical Analysis of Rhinovirus RNA Load Quantification by Real Time Reverse Transcription PCR
... two-step real-time RT-PCR assay, named Panenterhino/Ge/08, which could detect all known HRV genotypes and, to a lesser extent, human respiratory enteroviruses (HEVs) ...one-step quantitative ...
5
The differential effects of leukocyte-containing and pure platelet-rich plasma (PRP) on tendon stem/progenitor cells - implications of PRP application for the clinical treatment of tendon injuries
... using real-time qRT-PCR To determine the effects of L-PRP and P-PRP on the gene expression in tendon cells, we used real-time quantitative reverse ...
13
Sensitive Detection of Multiple Rotavirus Genotypes with a Single Reverse Transcription Real Time Quantitative PCR Assay
... Two reverse and five for- ward primers, together with a degenerate minor groove binding (MGB) TaqMan probe, were designed in the most conserved area (the 3⬘ end of the VP2 gene) with the help of Primer Express ...
8
Broadly Reactive and Highly Sensitive Assay for Norwalk Like Viruses Based on Real Time Quantitative Reverse Transcription PCR
... on reverse transcription- PCR (RT-PCR) that is highly sensitive to a broad range of ...a real-time quantitative RT-PCR ...
10
Design and Validation of an H5 TaqMan Real Time One Step Reverse Transcription PCR and Confirmatory Assays for Diagnosis and Verification of Influenza A Virus H5 Infections in Humans
... TaqMan real-time one-step RT-PCR ...TaqMan real-time assay by using Primer Express software (Applied Biosystems, Cheshire, United ...H5 real-time RT-PCR assay was ...
9
Rapid Detection and Differentiation of Dengue Virus Serotypes by a Real Time Reverse Transcription Loop Mediated Isothermal Amplification Assay
... one-step, real-time, and quantitative dengue virus serotype-specific reverse transcription–loop-mediated isothermal amplification (RT-LAMP) assay targeting the 3 ⴕ noncoding region for ...
9
Hepatitis E Virus (HEV) Detection and Quantification by a Real Time Reverse Transcription PCR Assay Calibrated to the World Health Organization Standard for HEV RNA
... A real-time reverse transcription-quantitative PCR (RT-qPCR) assay utilizing Pleiades probe chemistry and an RNA internal control for the simultaneous detection and quantification ...
10
The Lipopolysaccharide and β-1,3-Glucan Binding Protein Gene Is Upregulated in White Spot Virus-Infected Shrimp (Penaeus stylirostris)
... by real-time quantitative reverse transcription-PCR, and we showed that LGBP gene expression in shrimp was upregulated as the WSV infection ...
12
Selection and validation of reference genes for normalization of quantitative real-time reverse transcription PCR analysis in Poria cocos (Schw.) Wolf (Fuling)
... The liquid nitrogen frozen samples were ground into fine powder by a mortar and pestle. The total RNA of each sample was extracted by the Polysaccharide and Polyphe- nol Total RNA Isolation Kit (spin column; BioTeke, ...
17
miR-486-5p suppresses prostate cancer metastasis by targeting Snail and regulating epithelial–mesenchymal transition
... C4-2. Quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR) results demon- strated that the metastatic C-4 cell line showed significantly decreased ...
6
Single-base resolution of mouse offspring brain methylome reveals epigenome modifications caused by gestational folic acid
... genome from the HMFA group (Additional file 2: Table S2, Additional file 3: Table S3, Additional file 9: Table S4, Additional file 4: Table S5, and Additional file 5: Table S6). For example, zinc finger proteins Zfp608 ...
15
Shedding and Reversion of Oral Polio Vaccine Type 3 in Mexican Vaccinees: Comparison of Mutant Analysis by PCR and Enzyme Cleavage to a Real Time PCR Assay
... Real-time PCR is more sensitive than conventional PCR (4), and quantitation is recorded during the exponential phase of a PCR when ample reagents and templates are ...a ...
7