reverse transcription RT-PCR
Identification of Genetic Evidence for Dobrava Virus Spillover in Rodents by Nested Reverse Transcription (RT) PCR and TaqMan RT PCR
... TaqMan reverse transcription (RT)-PCR and for hantavirus RNA with the degenerate primers for nested PCR published by Scharninghausen et ...TaqMan RT-PCR, we used 500 nM ...
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Use of Bacteriophage MS2 as an Internal Control in Viral Reverse Transcription PCR Assays
... on reverse transcription (RT)-PCR are widely used for the detection of viral genomes in different human ...real-time RT-PCRs utilizing the coliphage MS2 replicase gene, which differ in ...
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Enhanced Reverse Transcription PCR Assay for Detection of Norovirus Genogroup I
... one-tube reverse transcription (RT)-PCR method using the real-time TaqMan PCR system for the detection of norovirus genogroup I (NV ...GGI RT-PCR was determined by probit ...
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Rapid Detection and Differentiation of Dengue Virus Serotypes by a Real Time Reverse Transcription Loop Mediated Isothermal Amplification Assay
... by reverse transcription (RT)-PCR, nested PCR, nucleic acid sequence-based amplification (NASBA), and real-time PCR are therefore assumed to be significant for the rapid ...
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Rapid Identification of Nine Microorganisms Causing Acute Respiratory Tract Infections by Single Tube Multiplex Reverse Transcription PCR: Feasibility Study
... as PCR (29) and reverse transcription (RT)-PCR (RT- PCR) are highly sensitive techniques for the detection of nu- cleic acid sequences from viruses and bacteria in ...
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Novel PCR-ELISA Technique as a Good Substitute in Molecular Assay
... usual PCR, after that, ELISA is performed to receive desired ...time. PCR-ELISA does not require using dangerous and carcinogenic material that have used in many of tech- niques, including of methylene blue ...
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Design and Validation of an H5 TaqMan Real Time One Step Reverse Transcription PCR and Confirmatory Assays for Diagnosis and Verification of Influenza A Virus H5 Infections in Humans
... real-time RT-PCR assay was shown to be extremely sensitive for the detection of influenza A H5 viruses, detecting clade 1, 2, and 3 H5 virus levels of 1 PFU or less by using a QuantiTect Probe ...
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Comparison of Commercial Real Time Reverse Transcription PCR Assays for Reliable, Early, and Rapid Detection of Heterologous Strains of Porcine Reproductive and Respiratory Syndrome Virus in Experimentally Infected or Noninfected Boars by Use of Different Sample Types
... Active PRRSV surveillance in boar studs relies mainly on col- lection and testing of serum, semen, blood swabs, and, more re- cently, oral fluids (6, 7). The choice of sample type should take into consideration the ...
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Multiplex reverse transcription PCR for surveillance of influenza A and B viruses in England and Wales in 1995 and 1996
... (multiplex) reverse transcription-PCR (RT-PCR) for detection, typing, and subtyping of the hemagglutinin gene of influenza type A (H3N2 and H1N1) and type B viruses was developed and ...
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Preclinical Evaluation of Two Real Time, Reverse Transcription PCR Assays for Detection of the Severe Acute Respiratory Syndrome Coronavirus
... We verified the analytical performance characteristics of a previously described real-time reverse transcription-PCR RT-PCR assay targeting the open reading frame ORF 1b region of the se[r] ...
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Performance and Cost Evaluation of One Commercial and Six In House Conventional and Real Time Reverse Transcription PCR Assays for Detection of Severe Acute Respiratory Syndrome Coronavirus
... in-house RT-PCR assays were evaluated for the detection of SARS-CoV ...the RT step combined with the first round of PCR amplification, followed by a second round of PCR ...nested ...
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DETECTION OF VIABLE SALMONELLA TYPHI USING THREE PRIMER PAIRS SPESCIFIC TO INVA, IVAB, AND FLIC-D GENES
... antigen (or H1-d antigen), the prt gene (previously known as rfbS), the gene encoding paratose synthase, the tyv gene (previously known as rfbE), the gene encoding CDP- tyvelose epimerase, and the ViaB region, a ...
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Cloning of a muscle specific calpain from the American lobster Homarus americanus: expression associated with muscle atrophy and restoration during moulting
... Here we report the cloning of a crustacean calpain gene with features that distinguish it from calpains isolated from other species. The cDNA encodes a non-EF-hand calpain with a unique N-terminal sequence and an ...
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Intestinal transcriptional profiling reveals fava bean-induced immune response in DBA/1 mice
... interleukin; RT-PCR: reverse transcription PCR; DAVID: database for annotation, visualization and integrated discovery; DEGs: differentially expressed genes; GO: gene ontology; IBD: ...
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Use of FTA Cards To Transport Throat Swabs and Oral Fluid Samples for Molecular Detection and Genotyping of Measles and Rubella Viruses
... by reverse transcription-quantitative real-time PCR (RT-qPCR) or the success of genotyping of MeV from samples stored on FTA cards compared to that for TS or oral fluid (OF) samples that were ...
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Developing a Sustainable Insect Pest Management System by Manipulating Plant Volatile Emissions
... levels, RT-qPCR experiments were conducted on the transgenic ...for reverse transcription with iScript Reverse Transcription Supermix (Bio-Rad Laboratories, Mississauga, ...Quantitative ...
114
Hepatitis D virus RNA editing: specific modification of adenosine in the antigenomic RNA.
... of transcription driven by the CMV promoter, it is possible that low levels of opposite-sense transcripts were pro- duced either from endogenous promoters present in the trans- fected HDV cDNA (22) or from low ...
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Multiplex reverse transcriptase pcr assay for the detection of infective stage wuchereria bancrofti and its vector culex quinquefasciatus
... There is currently a global program attempting to eliminate lymphatic filariasis (LF) by administering drugs to affected communities with the goal of interrupting transmission of the parasite. At present the c ...
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Superiority of Digital Reverse Transcription PCR (RT PCR) over Real Time RT PCR for Quantitation of Highly Divergent Human Rhinoviruses
... by RT-PCR is currently sufficient for deter- mining HRV infection, accurate quantification of HRV RNA in clinical samples is needed for studies associating HRV viral load with viral transmission and with ...
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In silico prioritization and further functional characterization of SPINK1 intronic variants
... performed RT-PCR to amplify the full-length transcripts prepared from the ...μl PCR mixture, which contained ...and reverse primer sequences are located within the beginning and the end ...
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