RFLP – restriction fragment length polymorphism
Microbial Diversity in Raw and Pasteurized Milk with Terminal Restriction Fragment Length Polymorphism (T-RFLP)
... Interestingly, there are TRFs appearing after treatment that were not identified in the raw milk. These were different from each other between the pasteurization techniques and also between the two sampling occasions. ...
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Restriction fragment length polymorphism (RFLP) analysis of Blastocystis spp. in symptomatic and asymptomatic individuals from Alexandria, Egypt
... PCR-RELP analysis of Blastocystis subtypes was performed in the present study on DNA extracted directly from fecal samples. It was previously observed that this method is better than genotyping based on DNA extracted ...
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IS900 Restriction Fragment Length Polymorphism (RFLP) Analysis of Mycobacterium avium subsp. paratuberculosisIsolates from Goats and Cattle in Norway
... benyttes RFLP metoden til ge- notypisk karakterisering av 51 isolat fra Norske gei- ter og 4 isolat fra Norske ...samme RFLP mønster (B-C1). Fem isolat hadde RFLP mønster som ikke tidligere er blitt ...
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Evaluation of PCR Restriction Profile Analysis and IS2404 Restriction Fragment Length Polymorphism and Amplified Fragment Length Polymorphism Fingerprinting for Identification and Typing of Mycobacterium ulcerans andM marinum
... PCR restriction profile analysis (PRPA), IS2404 restriction fragment length polymorphism (RFLP), and amplified fragment length polymorphism (AFLP) on a set ...
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Restriction fragment length polymorphism analysis of clinical isolates of Mycobacterium haemophilum
... The identical RFLP patterns observed among different isolates that were collected over many years (16 years in the case of the type 2 clone) and from the same patient (patient 12) over 3[r] ...
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Identification and Typing of Leptospira spp. by Restriction Fragment Length Polymorphism (RFLP) of 16S Gene rDNA and Pulsed-field Gel Electrophoresis (PFGE)
... Znanstveni rad Ukupno 13 izolata bakterije roda Leptospira izdvojenih iz doma}ih i divljih `i- votinja iz kontinentalnog dijela Republike Hrvatske identificirano je najprije se- rolo{ki metodom mikroskopske aglutinacije ...
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Differentiation of Mycobacterium tuberculosis isolates by spoligotyping and IS6110 restriction fragment length polymorphism
... formed with extracts of sputum or bronchoalveolar lavage sam- ples. This provides the clinician with information on whether the patient has been infected by nosocomial transmission of a particular strain or strains known ...
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Restriction Fragment Length Polymorphism Analysis of Some Flagellin Genes of Salmonella enterica
... to restriction fragment length polymorphism (RFLP) analysis by using endonucleases HhaI and ...HphI. RFLP with HhaI and HphI yielded 64 and 42 different restriction ...
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Species Identification of Mycobacteria by PCR Restriction Fragment Length Polymorphism of therpoB Gene
... sibly, strain specific. If strains of a certain species also have polymorphic RFLP profiles, the use of this method for myco- bacterial species identification will be very complex. Therefore, in a blinded test, ...
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Capillary Electrophoretic Restriction Fragment Length Polymorphism Patterns for the Mycobacterial hsp65 Gene
... two restriction enzymes (BstEII and HaeIII) and the separation of restriction fragments by 3% agarose gel ...However, restriction fragments shorter than 60 bp were not available in the diagnostic ...
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Genetic Identification of the Main Opportunistic Mucorales by PCR Restriction Fragment Length Polymorphism
... In this study we therefore described the development of a molecular technique by direct PCR-RFLP on histopathologi- cal samples to rapidly identify the main Mucorales reported to cause infections in humans. The ...
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Identification of Infectious Agents in Onychomycoses by PCR Terminal Restriction Fragment Length Polymorphism
... The PCR-TRFLP assay described here is simple, reliable, and suitable for dermatology laboratories provided that enough nail material is collected for analysis. In practice, we are using TRFLP for fungal identification ...
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Restriction fragment length polymorphism of the periplasmic flagellar flaA1 gene of Serpulina species.
... by restriction fragment length polymorphism (RFLP) of the periplasmic flagellar (PF) flaA1 ...unique RFLP fingerprint pattern, were identified by Southern blotting analysis of ...
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Determination on Yellow Fin Tuna Stock (Thunnus albacares) In South Java Sea Based on Genetic Variation by Restriction Fragment Length Polymorphism (RFLP) Method
... average length fish had been caught, and also tuna fishing grounds are getting ...Benoa). Restriction Fragment Length Polymorphism (RFLP) technique were applied for Cytochrome ...
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Pros and cons of ion-torrent next generation sequencing versus terminal restriction fragment length polymorphism T-RFLP for studying the rumen bacterial community
... Discussion High-throughput sequencing has allowed biologists to explore new ways to study sequence-based profiling and metagenomics in complex microbial communities, including those associated with human health and ...
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RESTRICTION FRAGMENT LENGTH POLYMORPHISM ANALYSIS OF RHIZOBIUM SP ISOLATED FROM DOLICHOUS LABLAB
... kbp. RFLP assay with restriction endonuclease EcoRI used to distinguish among the three ...This RFLP assay provides an inexpensive and simple means of identifying the Rhizobium ...sp. RFLP ...
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Discrimination of Human Pathogenic Subspecies of Francisella tularensis by Using Restriction Fragment Length Polymorphism
... In this study an alternative view of the organization of the genomes from different strains of F. tularensis is offered. Entire genomes can be compared with each other on the basis of the numbers and distributions of IS ...
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Detection of Cryptosporidium and Identification to the Species Level by Nested PCR and Restriction Fragment Length Polymorphism
... and restriction fragment length polymorphism (RFLP) that offers simple identification of Cryptosporidium hominis, Cryptosporidium parvum, and most other human infective species in stool ...
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Identification of Staphylococcus spp by PCR Restriction Fragment Length Polymorphism Analysis of dnaJ Gene
... On the other hand, PCR-RFLP analysis is useful for the taxonomy of staphylococci and proves to be easier, less expen- sive, and less equipment dependent than sequencing. Recently, PCR-RFLP analysis of the ...
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Prevalence and Genotyping of Trichomonas vaginalis Infected to dsRNA Virus by PCR–Restriction Fragment Length Poly-morphism (RFLP)
... In a study, polymorphism of T. vaginalis was evaluated by PCR-RAPD and position of T. vaginalis isolates in phylogenic tree and genetic diversity between different isolates has been studied. The result showed ...
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